Mono(2-ethyl-5-hydroxyhexyl) phthalate (Synonyms: MEHHP)

Cat. No.: HY-133677 Purity: 99.03%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

Mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) is an oxidative metabolite of Di(2-ethylhexyl) phthalate (DEHP). Mono(2-ethyl-5-hydroxyhexyl) phthalate may protective sperm DNA damage. Di(2-ethylhexyl) phthalate is the predominant plasticizer added to rigid polyvinyl chloride (PVC) to impart flexibility, temperature tolerance, optical clarity, strength and resistance to kinking.

For research use only. We do not sell to patients.

Mono(2-ethyl-5-hydroxyhexyl) phthalate Chemical Structure

CAS No. : 40321-99-1

Size	Price	Stock	Quantity
5 mg	USD 155	In-stock	Estimated Time of Arrival: April 21
10 mg	USD 250	In-stock	Estimated Time of Arrival: April 21
25 mg	USD 500	In-stock	Estimated Time of Arrival: April 21
50 mg	USD 800	In-stock	Estimated Time of Arrival: April 21
100 mg	USD 1280	In-stock	Estimated Time of Arrival: April 21
200 mg		Get quote
500 mg		Get quote

Get it by April 25 for select sizes. Order within 16 hrs 45 mins.

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Other Forms of Mono(2-ethyl-5-hydroxyhexyl) phthalate:

Mono(2-ethyl-5-hydroxyhexyl) phthalate-d₄ Get quote

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Description

Molecular Weight

294.34

Formula

C₁₆H₂₂O₅

CAS No.

40321-99-1

Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=C(C1=CC=CC=C1C(O)=O)OCC(CC)CCC(O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (339.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3974 mL	16.9872 mL	33.9743 mL
5 mM	0.6795 mL	3.3974 mL	6.7949 mL
10 mM	0.3397 mL	1.6987 mL	3.3974 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 99.03%

Select Batch:

Data Sheet (271 KB) SDS (392 KB)

COA (247 KB) HNMR (136 KB) RP-HPLC (243 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Hauser R. Urinary phthalate metabolites and semen quality: a review of a potential biomarker of susceptibility. Int J Androl. 2008;31(2):112-117. [Content Brief]

[2]. Van Vliet ED, et al. A review of alternatives to di (2-ethylhexyl) phthalate-containing medical devices in the neonatal intensive care unit [published correction appears in J Perinatol. 2012 Aug;32(8):648]. J Perinatol. 2011;31(8):551-560. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3974 mL	16.9872 mL	33.9743 mL	84.9358 mL
	5 mM	0.6795 mL	3.3974 mL	6.7949 mL	16.9872 mL
	10 mM	0.3397 mL	1.6987 mL	3.3974 mL	8.4936 mL
	15 mM	0.2265 mL	1.1325 mL	2.2650 mL	5.6624 mL
	20 mM	0.1699 mL	0.8494 mL	1.6987 mL	4.2468 mL
	25 mM	0.1359 mL	0.6795 mL	1.3590 mL	3.3974 mL
	30 mM	0.1132 mL	0.5662 mL	1.1325 mL	2.8312 mL
	40 mM	0.0849 mL	0.4247 mL	0.8494 mL	2.1234 mL
	50 mM	0.0679 mL	0.3397 mL	0.6795 mL	1.6987 mL
	60 mM	0.0566 mL	0.2831 mL	0.5662 mL	1.4156 mL
	80 mM	0.0425 mL	0.2123 mL	0.4247 mL	1.0617 mL
	100 mM	0.0340 mL	0.1699 mL	0.3397 mL	0.8494 mL

Mono(2-ethyl-5-hydroxyhexyl) phthalate Related Classifications

Endocrinology

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?
1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.
How should solution-form recombinant proteins be stored?
1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that
Why is it necessary to add carrier proteins?
Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.
Carrier protein types and options?
In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

Mono(2-ethyl-5-hydroxyhexyl) phthalate40321-99-1MEHHPDrug MetaboliteOxidativemetaboliteplasticizerInhibitorinhibitorinhibit

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Mono(2-ethyl-5-hydroxyhexyl) phthalate (Synonyms: MEHHP)

Other Forms of Mono(2-ethyl-5-hydroxyhexyl) phthalate:

Featured Recommendations

•Related Screening Libraries:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator