Cathepsin K inhibitor 6

Cat. No.: HY-147774 Purity: 98.18%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Cathepsin K inhibitor 6 (compound 19) is an inhibitor of cathepsin K (Cat K) with an IC₅₀ of 17 nM. Cathepsin K inhibitor 6 also has inhibitory effects on other isoforms, with IC₅₀s of 0.05 μM (Cat L) and 0.3 μM (Cat B), respectively.

For research use only. We do not sell to patients.

Cathepsin K inhibitor 6 Chemical Structure

CAS No. : 325775-42-6

Size	Price	Stock	Quantity
5 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 455	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1230	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cathepsin Isoform Specific Products:

View All Isoforms

Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

cathepsin L

0.05 μM (IC₅₀)

Cathepsin B

0.3 μM (IC₅₀)

cathepsin K

17 nM (IC₅₀)

Molecular Weight

251.30

Formula

C₁₁H₁₃N₃O₂S

CAS No.

325775-42-6

Appearance

Solid

Color

White to off-white

SMILES

N#CN1CC(C1)CNS(=O)(C2=CC=CC=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (397.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9793 mL	19.8965 mL	39.7931 mL
5 mM	0.7959 mL	3.9793 mL	7.9586 mL
10 mM	0.3979 mL	1.9897 mL	3.9793 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.95 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.95 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (9.95 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.18%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (248 KB) HNMR (134 KB) RP-HPLC (139 KB) LCMS (387 KB)

Handling Instructions (2659 KB)

References

[1]. Falgueyret JP, et al. Novel, nonpeptidic cyanamides as potent and reversible inhibitors of human cathepsins K and L. J Med Chem. 2001 Jan 4;44(1):94-104. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9793 mL	19.8965 mL	39.7931 mL	99.4827 mL
	5 mM	0.7959 mL	3.9793 mL	7.9586 mL	19.8965 mL
	10 mM	0.3979 mL	1.9897 mL	3.9793 mL	9.9483 mL
	15 mM	0.2653 mL	1.3264 mL	2.6529 mL	6.6322 mL
	20 mM	0.1990 mL	0.9948 mL	1.9897 mL	4.9741 mL
	25 mM	0.1592 mL	0.7959 mL	1.5917 mL	3.9793 mL
	30 mM	0.1326 mL	0.6632 mL	1.3264 mL	3.3161 mL
	40 mM	0.0995 mL	0.4974 mL	0.9948 mL	2.4871 mL
	50 mM	0.0796 mL	0.3979 mL	0.7959 mL	1.9897 mL
	60 mM	0.0663 mL	0.3316 mL	0.6632 mL	1.6580 mL
	80 mM	0.0497 mL	0.2487 mL	0.4974 mL	1.2435 mL
	100 mM	0.0398 mL	0.1990 mL	0.3979 mL	0.9948 mL