Ophthalmic acid

Name: Ophthalmic acid
Brand: MedChemExpress
SKU: HY-126752
Rating: 4.5 (1 reviews)

Cat. No.: HY-126752 Purity: 99.17%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ophthalmic acid, an analogue of GSH, is a marker of oxidative stress and hepatic GSH consumption. Ophthalmic acid is an inhibitor of Glyoxalase I reaction.

For research use only. We do not sell to patients.

Ophthalmic acid Chemical Structure

CAS No. : 495-27-2

Size	Price	Stock	Quantity
5 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Ophthalmic acid, an analogue of GSH, is a marker of oxidative stress and hepatic GSH consumption. Ophthalmic acid is an inhibitor of Glyoxalase I reaction^[1]^[2].

Molecular Weight

289.29

Formula

C₁₁H₁₉N₃O₆

CAS No.

495-27-2

Appearance

Solid

Color

White to off-white

SMILES

OC(CNC([C@H](CC)NC(CC[C@H](N)C(O)=O)=O)=O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

animal kingdom

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (345.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (345.67 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4567 mL	17.2837 mL	34.5674 mL
5 mM	0.6913 mL	3.4567 mL	6.9135 mL
10 mM	0.3457 mL	1.7284 mL	3.4567 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.17%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (244 KB) HNMR (263 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Gurnit Kaur, et al. Detection of Ophthalmic Acid in Serum from Acetaminophen-Induced Acute Liver Failure Patients Is More Frequent in Non-Survivors. PLoS One. 2015 Sep 25;10(9):e0139299. [Content Brief]

[2]. E E CLIFFE, et al. The mechanism of the glyoxalase I reaction, and the effect of ophthalmic acid as an inhibitor. Biochem J. 1961 Jun;79(3):475-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.4567 mL	17.2837 mL	34.5674 mL	86.4185 mL
	5 mM	0.6913 mL	3.4567 mL	6.9135 mL	17.2837 mL
	10 mM	0.3457 mL	1.7284 mL	3.4567 mL	8.6418 mL
	15 mM	0.2304 mL	1.1522 mL	2.3045 mL	5.7612 mL
	20 mM	0.1728 mL	0.8642 mL	1.7284 mL	4.3209 mL
	25 mM	0.1383 mL	0.6913 mL	1.3827 mL	3.4567 mL
	30 mM	0.1152 mL	0.5761 mL	1.1522 mL	2.8806 mL
	40 mM	0.0864 mL	0.4321 mL	0.8642 mL	2.1605 mL
	50 mM	0.0691 mL	0.3457 mL	0.6913 mL	1.7284 mL
	60 mM	0.0576 mL	0.2881 mL	0.5761 mL	1.4403 mL
	80 mM	0.0432 mL	0.2160 mL	0.4321 mL	1.0802 mL
	100 mM	0.0346 mL	0.1728 mL	0.3457 mL	0.8642 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Ophthalmic acid Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ophthalmic acid495-27-2Reactive Oxygen SpeciesGSHoxidative stressGlyoxalaseInhibitorinhibitorinhibit

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Ophthalmic acid

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Complete Stock Solution Preparation Table

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