p53 and MDM2 proteins-interaction-inhibitor (chiral)

Cat. No.: HY-70027 Purity: 98.13%: FAQs
Data Sheet SDS COA Handling Instructions

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p53 and MDM2 proteins-interaction-inhibitor (chiral) (Compound 32) is an inhibitor of the interaction between p53 and MDM2 proteins.

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p53 and MDM2 proteins-interaction-inhibitor (chiral) Chemical Structure

CAS No. : 939981-37-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 638	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 638	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 374	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2000	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Other Forms of p53 and MDM2 proteins-interaction-inhibitor (chiral):

p53 and MDM2 proteins-interaction-inhibitor dihydrochloride In-stock
p53 and MDM2 proteins-interaction-inhibitor (racemic) Get quote

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Biological Activity
Purity & Documentation
References
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Description

p53 and MDM2 proteins-interaction-inhibitor (chiral) (Compound 32) is an inhibitor of the interaction between p53 and MDM2 proteins.

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.6 μM Compound: 2k	Cytotoxicity against human HCT116 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay Cytotoxicity against human HCT116 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay	[PMID: 24900694]
MDA-MB-435	IC₅₀	17.5 μM Compound: 2k	Cytotoxicity against human MDA-MB-435 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay Cytotoxicity against human MDA-MB-435 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay	[PMID: 24900694]
RKO	IC₅₀	0.6 μM Compound: 2k	Cytotoxicity against human RKO cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay Cytotoxicity against human RKO cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay	[PMID: 24900694]
SJSA-1	IC₅₀	0.2 μM Compound: 2k	Cytotoxicity against human SJSA1 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay Cytotoxicity against human SJSA1 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay	[PMID: 24900694]
SW480	IC₅₀	14.4 μM Compound: 2k	Cytotoxicity against human SW480 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay Cytotoxicity against human SW480 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay	[PMID: 24900694]

Molecular Weight

734.75

Formula

C₄₀H₄₉Cl₂N₅O₄

CAS No.

939981-37-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCN(CC(N2CCOCC2)=O)CC1)N3[C@](C)(C4=CC=C(Cl)C=C4)[C@](C)(C5=CC=C(Cl)C=C5)N=C3C6=CC=C(C(C)(C)C)C=C6OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (68.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3610 mL	6.8050 mL	13.6101 mL
5 mM	0.2722 mL	1.3610 mL	2.7220 mL
10 mM	0.1361 mL	0.6805 mL	1.3610 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (4.42 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.25 mg/mL (4.42 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.13%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (255 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Chen R, et al. A Fusion Protein of the p53 Transaction Domain and the p53-Binding Domain of the Oncoprotein MdmX as an Efficient System for High-Throughput Screening of MdmX Inhibitors. Biochemistry. 2017 Jun 27;56(25):3273-3282. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3610 mL	6.8050 mL	13.6101 mL	34.0252 mL
	5 mM	0.2722 mL	1.3610 mL	2.7220 mL	6.8050 mL
	10 mM	0.1361 mL	0.6805 mL	1.3610 mL	3.4025 mL
	15 mM	0.0907 mL	0.4537 mL	0.9073 mL	2.2683 mL
	20 mM	0.0681 mL	0.3403 mL	0.6805 mL	1.7013 mL
	25 mM	0.0544 mL	0.2722 mL	0.5444 mL	1.3610 mL
	30 mM	0.0454 mL	0.2268 mL	0.4537 mL	1.1342 mL
	40 mM	0.0340 mL	0.1701 mL	0.3403 mL	0.8506 mL
	50 mM	0.0272 mL	0.1361 mL	0.2722 mL	0.6805 mL
	60 mM	0.0227 mL	0.1134 mL	0.2268 mL	0.5671 mL