2. Anti-infection Apoptosis
  3. Bacterial Antibiotic Apoptosis
  4. Phenazine methylsulfate

Phenazine methylsulfate  (Synonyms: 5-Methylphenazinium methylsulfate)

Cat. No.: HY-W004520 Purity: ≥98.0%
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Phenazine methylsulfate is a free radical generator that can act as an electron transfer reactant in cell viability assays. It also has insecticidal properties. Furthermore, Phenazine methylsulfate induces oxidative DNA damage and cell apoptosis, showing antitumor activity.

For research use only. We do not sell to patients.

Phenazine methylsulfate Chemical Structure

Phenazine methylsulfate Chemical Structure

CAS No. : 299-11-6

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Based on 1 publication(s) in Google Scholar

Other Forms of Phenazine methylsulfate:

Phenazine methylsulfate Related Antibodies

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1 Publications Citing Use of MCE Phenazine methylsulfate

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Description

Phenazine methylsulfate is a free radical generator that can act as an electron transfer reactant in cell viability assays. It also has insecticidal properties. Furthermore, Phenazine methylsulfate induces oxidative DNA damage and cell apoptosis, showing antitumor activity[1][2][3].

In Vitro

Phenazine methyl sulfate stimulates proton transport by promoting photosynthetic electron flow[1].
Phenazine methyl sulfate (10 μM, 2-24 h) can quickly disrupt and clear Leishmania amastigotes from macrophages without causing significant harm to the host cells[2].
Phenazine methyl sulfate (2.5-20 μM, 24 h) can inhibit or kill Leishmania promastigotes in a dose-dependent manner[2].
Phenazine methyl sulfate (0-10 μM, 24 h) induces apoptosis in A375, G361, and LOX cells[3].
Phenazine methyl sulfate (5-10 μM, 0-24 h) leads to the upregulation of heat shock, oxidative, and genotoxic stress response gene expression in A375 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phenazine methylsulfate Related Antibodies

Cell Viability Assay[2]

Cell Line: Macrophages infected with Leishmania m. amazonensis amastigotes
Concentration: 10 μM
Incubation Time: 2, 6, 24 h
Result: Reduced parasite vesicles.

Apoptosis Analysis[3]

Cell Line: A375, G361, LOX
Concentration: 0, 1, 5, 10 μM
Incubation Time: 24 h
Result: Induced cell death.

Western Blot Analysis[3]

Cell Line: A375
Concentration: 5, 10 μM
Incubation Time: 0, 1, 3, 6, 12, 18, 24 h
Result: Up-regulated the expression of Hsp70, antioxidant response regulatory transcription factors NRF2 and HO-1, up-regulated Bax, PUMA, cleaved PARP1, EGR1, and down-regulated Mcl-1, Bcl-2.
Molecular Weight

306.34

Formula

C14H14N2O4S
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

C[N+]1=C2C=CC=CC2=NC3=C1C=CC=C3.O=S(OC)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (326.43 mM; Need ultrasonic)

DMSO : 100 mg/mL (326.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2643 mL 16.3216 mL 32.6432 mL
5 mM 0.6529 mL 3.2643 mL 6.5286 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (6.79 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.08 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (326.43 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.2643 mL 16.3216 mL 32.6431 mL 81.6079 mL
5 mM 0.6529 mL 3.2643 mL 6.5286 mL 16.3216 mL
10 mM 0.3264 mL 1.6322 mL 3.2643 mL 8.1608 mL
15 mM 0.2176 mL 1.0881 mL 2.1762 mL 5.4405 mL
20 mM 0.1632 mL 0.8161 mL 1.6322 mL 4.0804 mL
25 mM 0.1306 mL 0.6529 mL 1.3057 mL 3.2643 mL
30 mM 0.1088 mL 0.5441 mL 1.0881 mL 2.7203 mL
40 mM 0.0816 mL 0.4080 mL 0.8161 mL 2.0402 mL
50 mM 0.0653 mL 0.3264 mL 0.6529 mL 1.6322 mL
60 mM 0.0544 mL 0.2720 mL 0.5441 mL 1.3601 mL
80 mM 0.0408 mL 0.2040 mL 0.4080 mL 1.0201 mL
100 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8161 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Phenazine methylsulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenazine299-11-65-MethylphenaziniumBacterialAntibioticApoptosisAnti-insectanti-tumorapoptosisA375Inhibitorinhibitorinhibit

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