Phytol (Synonyms: (E)-Phytol)

Name: Phytol
Brand: MedChemExpress
SKU: HY-N3075
Rating: 5.0 (2 reviews)

Cat. No.: HY-N3075 Purity: 98.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Phytol ((E)-Phytol) is an orally active diterpenoid alcohol that can be extracted from chlorophyll. Phytol has antioxidant, anti-inflammatory, anti-schistosomiasis and antibacterial activities.

For research use only. We do not sell to patients.

Phytol Chemical Structure

CAS No. : 150-86-7

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Liquid
1 g	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Phytol:

3,7,11,15-Tetramethyl-2-hexadecen-1-ol Get quote

2 Publications Citing Use of MCE Phytol

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Biological Activity
Purity & Documentation
References
Customer Review

Description

Phytol ((E)-Phytol) is an orally active diterpenoid alcohol that can be extracted from chlorophyll. Phytol has antioxidant, anti-inflammatory, anti-schistosomiasis and antibacterial activities^[1]^[2]^[3].

IC₅₀ & Target

Schistosome

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 5	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 25497963]
BMDC	IC₅₀	1.22 μM Compound: 19	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated TNF-alpha production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated TNF-alpha production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
BMDC	IC₅₀	2.02 μM Compound: 19	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL12 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL12 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
BMDC	IC₅₀	3.62 μM Compound: 19	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL-12p40 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL-12p40 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
HCT-15	IC₅₀	22.5 μM Compound: 3	Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]
HeLa	IC₅₀	46.6 μM Compound: 6	Cytotoxicity against human HeLa cells by MTT method Cytotoxicity against human HeLa cells by MTT method	[PMID: 17547457]
HL-60	IC₅₀	> 100 μM Compound: 5	Cytotoxicity against human HL60 cells by SRB assay Cytotoxicity against human HL60 cells by SRB assay	[PMID: 25497963]
K562	IC₅₀	45.8 μg/mL Compound: Phytol	Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 32527555]
MCF7	IC₅₀	> 50 μg/mL Compound: 18	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 21109433]
SW-620	IC₅₀	5.85 μM Compound: 3	Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]
U-251	IC₅₀	27.8 μM Compound: 3	Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]
U-87MG ATCC	IC₅₀	8.02 μM Compound: 3	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 25981689]

In Vitro

Phytol (50-100 μg/mL, 48 h) reduces the motor activity of worms and causes their death^[1].
Phytol (40 μM, 2 h) inhibits osteoclast differentiation and oxidative stress of RAW264.7 cells induced by RANKL by regulating Nrf2/HO-1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	RAW264.7
Concentration:	5, 10, 20, 40 μM
Incubation Time:	5 days
Result:	Did not exert toxicity to RAW264.7.

Western Blot Analysis^[2]

Cell Line:	RAW264.7
Concentration:	40 μM
Incubation Time:	2 h
Result:	Results: Decreased the Nrf2 expression in the cytosolic extract and increased in the nuclear extract. Did not affect the expression of the housekeeping genes, β-actin and lamin B. Reduced the expression of NFATc1 and c-Fos. Recovered the expression of SOD, CAT.

Cell Migration Assay ^[2]

Cell Line:	RAW264.7
Concentration:	5, 10, 20, 40 μM
Incubation Time:	5 days
Result:	Decreased cell migration and the bone resorption area and osteoclast movement.

In Vivo

Phytol (40 mg/kg, orally, for 2 weeks) can reduce the number of parasites in mice infected with adult C. mansoni^[1].
Phytol (10 or 20 mg/kg, orally, for 4 consecutive days) can improve anemia and oxidative brain damage in Plasmodium bergsii infected mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Plasmodium berghei infected mice^[3]
Dosage:	10 or 20 mg/kg
Administration:	p.o.
Result:	Suppressed the multiplication of P. berghei in a dose-dependent manner and ameliorated the P. berghei-induced anaemia and brain damage.

Molecular Weight

296.53

Formula

C₂₀H₄₀O

CAS No.

150-86-7

Appearance

Liquid (Density: 0.85 g/cm³)

Color

Yellow to brown

SMILES

CC(C)CCC[C@@H](C)CCC[C@@H](C)CCC/C(C)=C/CO

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (421.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (84.31 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3723 mL	16.8617 mL	33.7234 mL
5 mM	0.6745 mL	3.3723 mL	6.7447 mL
10 mM	0.3372 mL	1.6862 mL	3.3723 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.25 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.93%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (238 KB) HNMR (257 KB) RP-HPLC (215 KB) MS (71 KB) OR (249 KB)

Handling Instructions (2659 KB)

References

[1]. de Moraes J, et al. Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical diseaseSchistosomiasis mansoni. PLoS Negl Trop Dis. 2014 Jan 2;8(1):e2617. [Content Brief]

[2]. Kim EN, et al. Phytol Suppresses Osteoclast Differentiation and Oxidative Stress through Nrf2/HO-1 Regulation in RANKL-Induced RAW264.7 Cells. Cells. 2022 Nov 14;11(22):3596. [Content Brief]

[3]. Usman MA, et al. Phytol suppresses parasitemia and ameliorates anaemia and oxidative brain damage in mice infected with Plasmodium berghei. Exp Parasitol. 2021 May;224:108097. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.3723 mL	16.8617 mL	33.7234 mL	84.3085 mL
	5 mM	0.6745 mL	3.3723 mL	6.7447 mL	16.8617 mL
	10 mM	0.3372 mL	1.6862 mL	3.3723 mL	8.4309 mL
	15 mM	0.2248 mL	1.1241 mL	2.2482 mL	5.6206 mL
	20 mM	0.1686 mL	0.8431 mL	1.6862 mL	4.2154 mL
	25 mM	0.1349 mL	0.6745 mL	1.3489 mL	3.3723 mL
	30 mM	0.1124 mL	0.5621 mL	1.1241 mL	2.8103 mL
	40 mM	0.0843 mL	0.4215 mL	0.8431 mL	2.1077 mL
	50 mM	0.0674 mL	0.3372 mL	0.6745 mL	1.6862 mL
	60 mM	0.0562 mL	0.2810 mL	0.5621 mL	1.4051 mL
	80 mM	0.0422 mL	0.2108 mL	0.4215 mL	1.0539 mL
DMSO	100 mM	0.0337 mL	0.1686 mL	0.3372 mL	0.8431 mL

Phytol Related Classifications

Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phytol150-86-7(E)-PhytolParasiteBacterialKeap1-Nrf2Anti-inflammatoryAntioxidantRAW264.7Plasmodium bergheiInhibitorinhibitorinhibit

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Phytol (Synonyms: (E)​-​Phytol)

Customer Review

Other Forms of Phytol:

Phytol Related Antibodies

2 Publications Citing Use of MCE Phytol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Parasite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Phytol Related Antibodies

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Complete Stock Solution Preparation Table

Phytol Related Classifications

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Phytol (Synonyms: (E)-Phytol)