Pyridoxal hydrochloride

Name: Pyridoxal hydrochloride
Brand: MedChemExpress
SKU: HY-W027446
Rating: 4.5 (1 reviews)

Cat. No.: HY-W027446 Purity: 99.75%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Pyridoxal hydrochloride, the hydrochloride form of Pyridoxal (HY-107469), is a newly developed neuroprotective agent. Pyridoxal hydrochloride is one of the major forms of vitamin B6. Pyridoxal hydrochloride is solvolyzed in methanol to form pyridoxal monomethylacetal. Pyridoxal is phosphorylated by pyridoxal kinase to Pyridoxal phosphate (HY-B1744). Pyridoxal is oxidized by the liver to 4-Pyridoxic acid (HY-113493) which is excreted in the urine. Pyridoxal hydrochloride is proming for research of carpal tunnel syndrome (CTS).

For research use only. We do not sell to patients.

Pyridoxal hydrochloride Chemical Structure

CAS No. : 65-22-5

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Pyridoxal hydrochloride:

Pyridoxal Get quote
Pyridoxal hydrochloride (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Pyridoxal hydrochloride (5 μM) has an increasing effect on the activity of tyrosine decarboxylase^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

203.62

Formula

C₈H₁₀ClNO₃

CAS No.

65-22-5

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=CC1=C(C=NC(C)=C1O)CO.Cl

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (245.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (163.68 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9110 mL	24.5549 mL	49.1099 mL
5 mM	0.9822 mL	4.9110 mL	9.8220 mL
10 mM	0.4911 mL	2.4555 mL	4.9110 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (24.55 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (24.55 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (491.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (251 KB) HNMR (260 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Vrolijk MF, et al. The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function[J]. Toxicol In Vitro. 2017 Oct;44:206-212. [Content Brief]

[2]. Aufiero E, et al. Pyridoxine hydrochloride treatment of carpal tunnel syndrome: a review[J]. Nutr Rev. 2004 Mar;62(3):96-104. [Content Brief]

[3]. Nagata Y, et al. Solvolysis of pyridoxal hydrochloride in alcohols[J]. Chemical and pharmaceutical bulletin, 1993, 41(6): 1019-1022.

[4]. Vrolijk MF, et al. The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function. Toxicol In Vitro. 2017 Oct;44:206-212. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.9110 mL	24.5549 mL	49.1099 mL	122.7747 mL
	5 mM	0.9822 mL	4.9110 mL	9.8220 mL	24.5549 mL
	10 mM	0.4911 mL	2.4555 mL	4.9110 mL	12.2775 mL
	15 mM	0.3274 mL	1.6370 mL	3.2740 mL	8.1850 mL
	20 mM	0.2455 mL	1.2277 mL	2.4555 mL	6.1387 mL
	25 mM	0.1964 mL	0.9822 mL	1.9644 mL	4.9110 mL
	30 mM	0.1637 mL	0.8185 mL	1.6370 mL	4.0925 mL
	40 mM	0.1228 mL	0.6139 mL	1.2277 mL	3.0694 mL
	50 mM	0.0982 mL	0.4911 mL	0.9822 mL	2.4555 mL
	60 mM	0.0818 mL	0.4092 mL	0.8185 mL	2.0462 mL
	80 mM	0.0614 mL	0.3069 mL	0.6139 mL	1.5347 mL
	100 mM	0.0491 mL	0.2455 mL	0.4911 mL	1.2277 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.