RBPJ Inhibitor-1 (Synonyms: RIN1)

Name: RBPJ Inhibitor-1
Brand: MedChemExpress
SKU: HY-137471
Rating: 5.0 (3 reviews)

Cat. No.: HY-137471 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

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RBPJ Inhibitor-1 (RIN1), the first RBPJ inhibitor, blocks the functional interaction of RBPJ with SHARP. RBPJ Inhibitor-1 (RIN1) inhibits NOTCH-dependent tumor cell proliferation.

For research use only. We do not sell to patients.

RBPJ Inhibitor-1 Chemical Structure

CAS No. : 2682114-39-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE RBPJ Inhibitor-1

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Description

RBPJ Inhibitor-1 (RIN1), the first RBPJ inhibitor, blocks the functional interaction of RBPJ with SHARP. RBPJ Inhibitor-1 (RIN1) inhibits NOTCH-dependent tumor cell proliferation^[1].

In Vitro

RIN1 inhibits the proliferation of hematologic cancer cell lines and promoted skeletal muscle diferentiation from C2C12 myoblasts^[1].
RIN1 (0.6 μM, corresponding to 3 × IC₅₀) decreases the number of MHC+ cells and increased the number of nuclei per cell, indicating that it induces the formation of multinucleated myofbers^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	AD-293 cells were transfected with RBPJ-VP16myc.
Concentration:	2 μM.
Incubation Time:	17 hours.
Result:	Inhibited Hes1-Luciferase activity with an IC₅₀ of 0.18 µM and E_max of 82%. Inhibited NOTCH3 ICD with similar potency and efcacy (0.19 µM and E_max=88%).

Cell Proliferation Assay^[1]

Cell Line:	T-cell acute lymphoblastic leukemia (T-ALL) patients (Jurkat and KOPT-K1) and in the mantle cell lymphoma (MCL) line REC-1.
Concentration:	0.1-10 μM.
Incubation Time:	96 h.
Result:	Inhibited NOTCH-dependent tumor cell proliferation. Was active in both the cancer cell anti-proliferation and myoblast diferentiation assays.

Molecular Weight

311.31

Formula

C₁₇H₁₄FN₃O₂

CAS No.

2682114-39-0

Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C(N)C1=CC=C(C2=CC=NN2C)C=C1OC3=CC=CC=C3F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (803.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2122 mL	16.0612 mL	32.1223 mL
5 mM	0.6424 mL	3.2122 mL	6.4245 mL
10 mM	0.3212 mL	1.6061 mL	3.2122 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (255 KB) HNMR (254 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Cecilia Hurtado, et al. Disruption of NOTCH signaling by a small molecule inhibitor of the transcription factor RBPJ. Sci Rep. 2019 Jul 25;9(1):10811. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2122 mL	16.0612 mL	32.1223 mL	80.3058 mL
	5 mM	0.6424 mL	3.2122 mL	6.4245 mL	16.0612 mL
	10 mM	0.3212 mL	1.6061 mL	3.2122 mL	8.0306 mL
	15 mM	0.2141 mL	1.0707 mL	2.1415 mL	5.3537 mL
	20 mM	0.1606 mL	0.8031 mL	1.6061 mL	4.0153 mL
	25 mM	0.1285 mL	0.6424 mL	1.2849 mL	3.2122 mL
	30 mM	0.1071 mL	0.5354 mL	1.0707 mL	2.6769 mL
	40 mM	0.0803 mL	0.4015 mL	0.8031 mL	2.0076 mL
	50 mM	0.0642 mL	0.3212 mL	0.6424 mL	1.6061 mL
	60 mM	0.0535 mL	0.2677 mL	0.5354 mL	1.3384 mL
	80 mM	0.0402 mL	0.2008 mL	0.4015 mL	1.0038 mL
	100 mM	0.0321 mL	0.1606 mL	0.3212 mL	0.8031 mL

RBPJ Inhibitor-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RBPJ Inhibitor-1 (Synonyms: RIN1)

Customer Review

RBPJ Inhibitor-1 Related Antibodies

3 Publications Citing Use of MCE RBPJ Inhibitor-1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

RBPJ Inhibitor-1 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

RBPJ Inhibitor-1 Related Classifications

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