Rebaudioside M

Name: Rebaudioside M
Brand: MedChemExpress
SKU: HY-N6833
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6833 Purity: 99.61%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Rebaudioside M, a glycoside of the ent-kaurene diterpenoid aglycone, is a non-calorie sweetener isolated from Stevia rebaudiana. Rebaudioside M has antidiabetics, antihypertension, anti-inflammatory, antioxidant, anticaries and anticancer benefits. Rebaudioside M is more than 350 times sweeter than sucrose. Rebaudioside M can be used in food and beverage .

For research use only. We do not sell to patients.

Rebaudioside M Chemical Structure

CAS No. : 1220616-44-3

Size	Price	Stock	Quantity
1 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Rebaudioside M:

Rebaudioside M (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Rebaudioside M demonstrates to protect β-cell activity and enhance insulin secretion via increasing calcium influx into β-cells^[3].
Rebaudioside M inhibits the PI3K/Akt signaling pathway resulting in lower cell proliferation and enhanced apoptosis and downregulates the Wnt/catenin signaling pathway in colon cancer cells, which is linked to reduced cell migration and invasion^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rebaudioside M lowers fasting blood glucose levels and enhanced glucose tolerance in diabetic rats^[3].
Rebaudioside M decreases oxidative stress and the production of pro-inflammatory cytokines in diabetic rats, protecting against pancreatic beta-cell damage^[4].
Rebaudioside M reduces blood pressure via the suppression of angiotensin-converting enzyme, increases endothelial function and causes vasodilation in hypertensive rats^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

1291.29

Formula

C₅₆H₉₀O₃₃

CAS No.

1220616-44-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@]3([H])[C@@](C4)(CC[C@]1([H])[C@@](C)(CCC2)C(O[C@H]5[C@@H]([C@H]([C@H](O)[C@@H](CO)O5)O[C@]6([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]6O)CO)O[C@]7([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]7O)CO)=O)C[C@@](C4=C)(O[C@@]8([H])[C@@H]([C@H]([C@H](O)[C@@H](CO)O8)O[C@]9([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]9O)CO)O[C@]%10([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]%10O)CO)CC3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (77.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7744 mL	3.8721 mL	7.7442 mL
5 mM	0.1549 mL	0.7744 mL	1.5488 mL
10 mM	0.0774 mL	0.3872 mL	0.7744 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.61%

Select Batch:

Data Sheet (282 KB) SDS (252 KB)

COA (261 KB) HNMR (397 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)

References

[1]. Prakash I, et al. Development of Next Generation Stevia Sweetener: Rebaudioside M. Foods. 2014 Feb 27;3(1):162-175. [Content Brief]

[2]. Xiaoqiang W, et al. Fusion glycosyltransferase design for enhanced conversion of rebaudioside A into rebaudioside M in cascade. Molecular Catalysis. 2023 Aug, 547: 113317.

[3]. Jeppesen PB, et al. Stevioside acts directly on pancreatic beta cells to secrete insulin: actions independent of cyclic adenosine monophosphate and adenosine triphosphate-sensitive K+-channel activity. Metabolism. 2000 Feb;49(2):208-14. [Content Brief]

[4]. Jan S A, et al. The anti-diabetic activities of natural sweetener plant Stevia: an updated review[J]. SN Applied Sciences, 2021, 3: 1-6.

[5]. Chakraborty R, et al. Angiotensin-converting enzyme inhibitors from plants: A review of their diversity, modes of action, prospects, and concerns in the management of diabetes-centric complications[J]. Journal of Integrative Medicine, 2021, 19(6): 478-492. [Content Brief]

[6]. Iatridis N, et al. Anti-Cancer Properties of Stevia rebaudiana; More than a Sweetener. Molecules. 2022 Feb 17;27(4):1362. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.7744 mL	3.8721 mL	7.7442 mL	19.3605 mL
	5 mM	0.1549 mL	0.7744 mL	1.5488 mL	3.8721 mL
	10 mM	0.0774 mL	0.3872 mL	0.7744 mL	1.9360 mL
	15 mM	0.0516 mL	0.2581 mL	0.5163 mL	1.2907 mL
	20 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9680 mL
	25 mM	0.0310 mL	0.1549 mL	0.3098 mL	0.7744 mL
	30 mM	0.0258 mL	0.1291 mL	0.2581 mL	0.6453 mL
	40 mM	0.0194 mL	0.0968 mL	0.1936 mL	0.4840 mL
	50 mM	0.0155 mL	0.0774 mL	0.1549 mL	0.3872 mL
	60 mM	0.0129 mL	0.0645 mL	0.1291 mL	0.3227 mL

Rebaudioside M Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rebaudioside M1220616-44-3OthersSweetenerSucroseStevia rebaudianaInhibitorinhibitorinhibit

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Rebaudioside M

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