CXCR2 Protein, Human (Cell-Free, His)

Cat. No.: HY-P790083: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Protein solubilization and storage

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CXCR2, the receptor for interleukin-8 (IL-8), orchestrates neutrophil activation through a G-protein-mediated phosphatidylinositol-calcium second messenger system upon IL-8 binding. Exhibiting high-affinity binding to IL-8, CXCR2 also interacts with other ligands like CXCL3, GRO/MGSA, and NAP-2. The involvement of GNAI2 underscores the intricate signaling mechanisms regulating neutrophil function through CXCR2. CXCR2 Protein, Human (Cell-Free, His) is the recombinant human-derived CXCR2, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CXCR2 Protein, Human (Cell-Free, His) is 360 a.a.,

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Size	Price	Stock	Quantity
50 μg	USD 1000	In-stock	Estimated Time of Arrival: April 21
100 μg		Get quote

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Description	CXCR2, the receptor for interleukin-8 (IL-8), orchestrates neutrophil activation through a G-protein-mediated phosphatidylinositol-calcium second messenger system upon IL-8 binding. Exhibiting high-affinity binding to IL-8, CXCR2 also interacts with other ligands like CXCL3, GRO/MGSA, and NAP-2. The involvement of GNAI2 underscores the intricate signaling mechanisms regulating neutrophil function through CXCR2. CXCR2 Protein, Human (Cell-Free, His) is the recombinant human-derived CXCR2, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CXCR2 Protein, Human (Cell-Free, His) is 360 a.a.,
Background	CXCR2, the receptor for interleukin-8 (IL-8), a potent neutrophil chemotactic factor, orchestrates the activation of neutrophils upon IL-8 binding. This response is mediated through a G-protein, initiating a phosphatidylinositol-calcium second messenger system. CXCR2 exhibits high-affinity binding not only to IL-8 but also to other ligands such as CXCL3, GRO/MGSA, and NAP-2. The interaction with IL-8 and GNAI2 further highlights the intricate signaling mechanisms involved in the regulation of neutrophil function by CXCR2.
Species	Human
Source	E. coli Cell-free
Tag	N-10*His
Accession	P25025 (M1-L360)
Gene ID	3579
Molecular Construction	N-term 10*His CXCR2 (M1-L360) Accession # P25025 C-term
Synonyms	CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B (IL-8R B); IL-8 receptor type 2; CXCR2; IL8RB
AA Sequence	MEDFNMESDSFEDFWKGEDLSNYSYSSTLPPFLLDAAPCEPESLEINKYFVVIIYALVFLLSLLGNSLVMLVILYSRVGRSVTDVYLLNLALADLLFALTLPIWAASKVNGWIFGTFLCKVVSLLKEVNFYSGILLLACISVDRYLAIVHATRTLTQKRYLVKFICLSIWGLSLLLALPVLLFRRTVYSSNVSPACYEDMGNNTANWRMLLRILPQSFGFIVPLLIMLFCYGFTLRTLFKAHMGQKHRAMRVIFAVVLIFLLCWLPYNLVLLADTLMRTQVIQETCERRNHIDRALDATEILGILHSCLNPLIYAFIGQKFRHGLLKILAIHGLISKDSLPKDSRPSFVGSSSGHTSTTL
Molecular Weight	Observed band size: Monomer: 42 kDa Dimer: 84 kDa. It is speculated that the protein forms a dimeric structure.
Purity	Greater than 90% as determined by reducing SDS-PAGE.
Appearance	Lyophilized powder
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6%Trehalose, pH 7.4.
Endotoxin Level	<1 EU/μg, determined by LAL method.
Reconstitution	It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.
Storage & Stability	Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping	Room temperature in continental US; may vary elsewhere.
Documentation	Select Batch: Data Sheet (237 KB) SDS (251 KB) COA (206 KB) Handling Instructions (2659 KB)

CXCR2 Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?
1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.
How should solution-form recombinant proteins be stored?
1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that
Why is it necessary to add carrier proteins?
Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.
Carrier protein types and options?
In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Reconstitution Calculator
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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)	=	Mass (in vial)	÷	Desired Reconstitution Concentration
	=		÷

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 10⁶ ÷ Biological Activity (ED₅₀)

Specific Activity (Unit/mg)	=	10⁶	÷	Biological Activity (ED₅₀)
Unit/mg	=	10⁶	÷	ng/mL

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CXCR2 Protein, Human (Cell-Free, His)

Biological Activity

Technical Parameters

Properties

Documentation

CXCR2 Protein, Human (Cell-Free, His) Related Classifications

Reconstitution Calculator

Dilution Calculator

Specific Activity Calculator

The reconstitution calculator equation

The dilution calculator equation

The specific activity calculator equation

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