2. Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein Membrane Transporter/Ion Channel Anti-infection
  3. Endogenous Metabolite 5-HT Receptor mGluR iGluR Bacterial Antibiotic
  4. Roemerine

Roemerine  (Synonyms: (-)-Roemerine)

Cat. No.: HY-121793 Purity: 99.61%
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Roemerine is an alkaloid that has been identified from the leaves of Fibraurea recisa Pierre. Roemerine exhibits antibacterial, anticancer, and antidepressant activities, can reverse the multidrug resistance phenotype in cultured cells, and exerts antibacterial effects by regulating the cAMP signaling pathway. Additionally, Roemerine influences neuronal activity by increasing BDNF protein expression and modulating the serotonergic and glutamatergic systems. Roemerine holds promise for research in the fields of cancer, infections, and neurological diseases.

For research use only. We do not sell to patients.

Roemerine Chemical Structure

Roemerine Chemical Structure

CAS No. : 548-08-3

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Based on 1 publication(s) in Google Scholar

Roemerine Related Antibodies

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  • Biological Activity

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Description

Roemerine is an alkaloid that has been identified from the leaves of Fibraurea recisa Pierre. Roemerine exhibits antibacterial, anticancer, and antidepressant activities, can reverse the multidrug resistance phenotype in cultured cells, and exerts antibacterial effects by regulating the cAMP signaling pathway. Additionally, Roemerine influences neuronal activity by increasing BDNF protein expression and modulating the serotonergic and glutamatergic systems. Roemerine holds promise for research in the fields of cancer, infections, and neurological diseases[1][2][3][4][5].

Cellular Effect
Cell Line Type Value Description References
A-431 ED50
4.3 μg/mL
Compound: 1
Cytotoxicity against human A431 cells after 72 hrs by SRB assay
Cytotoxicity against human A431 cells after 72 hrs by SRB assay
[PMID: 7623038]
BC1 cell line ED50
8.3 μg/mL
Compound: 1
Cytotoxicity against human BC1 cells after 72 hrs by SRB assay
Cytotoxicity against human BC1 cells after 72 hrs by SRB assay
[PMID: 7623038]
Col2 ED50
5.8 μg/mL
Compound: 1
Cytotoxicity against human Col2 cells after 72 hrs by SRB assay
Cytotoxicity against human Col2 cells after 72 hrs by SRB assay
[PMID: 7623038]
HT ED50
6.4 μg/mL
Compound: 1
Cytotoxicity against human HT cells after 72 hrs by SRB assay
Cytotoxicity against human HT cells after 72 hrs by SRB assay
[PMID: 7623038]
KB-V1 ED50
> 20 μg/mL
Compound: 1
Cytotoxicity against human multidrug resistant KBV1 cells in absence of vinblastine after 72 hrs by SRB assay
Cytotoxicity against human multidrug resistant KBV1 cells in absence of vinblastine after 72 hrs by SRB assay
[PMID: 7623038]
LNCaP ED50
10.7 μg/mL
Compound: 1
Cytotoxicity against human LNCAP cells after 72 hrs by SRB assay
Cytotoxicity against human LNCAP cells after 72 hrs by SRB assay
[PMID: 7623038]
Lu1 ED50
6.6 μg/mL
Compound: 1
Cytotoxicity against human Lu1 cells after 72 hrs by SRB assay
Cytotoxicity against human Lu1 cells after 72 hrs by SRB assay
[PMID: 7623038]
P388 ED50
> 5 μg/mL
Compound: 1
Cytotoxicity against mouse P388 cells after 72 hrs by SRB assay
Cytotoxicity against mouse P388 cells after 72 hrs by SRB assay
[PMID: 7623038]
RAW264.7 IC50
1.39 μM
Compound: 130
Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay
Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay
[PMID: 33454546]
SK-MEL-2 ED50
> 20 μg/mL
Compound: 1
Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay
[PMID: 7623038]
U-373MG ATCC ED50
5 μg/mL
Compound: 1
Cytotoxicity against human U373 cells after 72 hrs by SRB assay
Cytotoxicity against human U373 cells after 72 hrs by SRB assay
[PMID: 7623038]
ZR-75-1 ED50
> 20 μg/mL
Compound: 1
Cytotoxicity against human ZR-75-1 cells after 72 hrs by SRB assay
Cytotoxicity against human ZR-75-1 cells after 72 hrs by SRB assay
[PMID: 7623038]
In Vitro

Roemerine (0-1 mM, 48 h) significantly reduces cell viability in RM-1 cells, with IC50 of 1177.4 µM [2].
Roemerine (8 µg/mL, 8 h) can influence biofilm formation in Candida albicans SC5314 through the regulation of the cAMP signaling pathway[3].
Roemerine (10 μM， 48 h) affects neuronal activity by increasing BDNF protein expression and modulating the serotonergic and glutamatergic systems in SH-SY5Y cells, exhibiting antidepressant-like effects[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Roemerine Related Antibodies

Cell Viability Assay[2]

Cell Line: RM-1 cells (prostate cancer, mouse)
Concentration: 0 µM, 1 µM, 2 µM, 5 µM, 10 µM, 20 µM, 50 µM, 100 µM, 200 µM, 500 µM, 1000 µM
Incubation Time: 48 h
Result: Significantly reduced RM-1 cell viability, with IC50 of 1177.4 µM.

RT-PCR[3]

Cell Line: Candida albicans SC5314
Concentration: 8 µg/mL
Incubation Time: 8 h
Result: Significantly upregulated YWP1, SAP5, SAP6, HWP1, and ECE1 genes while downregulating EFG1 gene.
In Vivo

Roemerine (30 mg/kg, i.p., once every three days for 30 days) significantly inhibited tumor growth in the prostate cancer BALB/C nude mouse model (established by subcutaneous implantation of RM-1 cells)[2].
Roemerine (20 mg/kg, i.p., once daily for 2 days) increases survival and reduced bacterial loads in multiple organs in the MRSA XJ75302-induced sepsis BALB/C mouse model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Prostate cancer BALB/C nude mouse model (established by subcutaneous implantation of RM-1 cells)[3]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection (i.p.), once every three days for 30 days
Result: Significantly reduced tumor volume and weight in the experimental group (P < 0.05), with an inhibition rate of 41.2%.
Animal Model: MRSA XJ75302-induced sepsis BALB/C mouse model[5]
Dosage: 20 mg/kg
Administration: Intraperitoneal injection (i.p.), once daily, for 2 days
Result: Increased the 7-day survival rate of mice to 58.3% (p < 0.05) and reduced bacterial loads in the liver (4.71 to 4.38 log CFU/mL), kidney (4.37 to 3.79 log CFU/mL), heart (5.44 to 3.76 log CFU/mL), and blood (6.52 to 4.55 log CFU/mL).
Note: CFU/mL represents the number of bacteria per milliliter of culture medium or tissue sample that can form colonies (CFU, Colony-Forming Units).
Molecular Weight

279.33

Formula

C18H17NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CN1CCC(C2=C3C4=CC=CC=C4C[C@@]12[H])=CC5=C3OCO5
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (358.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5800 mL 17.9000 mL 35.8000 mL
5 mM 0.7160 mL 3.5800 mL 7.1600 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5800 mL 17.9000 mL 35.8000 mL 89.4999 mL
5 mM 0.7160 mL 3.5800 mL 7.1600 mL 17.9000 mL
10 mM 0.3580 mL 1.7900 mL 3.5800 mL 8.9500 mL
15 mM 0.2387 mL 1.1933 mL 2.3867 mL 5.9667 mL
20 mM 0.1790 mL 0.8950 mL 1.7900 mL 4.4750 mL
25 mM 0.1432 mL 0.7160 mL 1.4320 mL 3.5800 mL
30 mM 0.1193 mL 0.5967 mL 1.1933 mL 2.9833 mL
40 mM 0.0895 mL 0.4475 mL 0.8950 mL 2.2375 mL
50 mM 0.0716 mL 0.3580 mL 0.7160 mL 1.7900 mL
60 mM 0.0597 mL 0.2983 mL 0.5967 mL 1.4917 mL
80 mM 0.0447 mL 0.2237 mL 0.4475 mL 1.1187 mL
100 mM 0.0358 mL 0.1790 mL 0.3580 mL 0.8950 mL
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Roemerine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Roemerine548-08-3(-)-RoemerineEndogenous Metabolite5-HT ReceptormGluRiGluRBacterialAntibioticSerotonin Receptor5-hydroxytryptamine ReceptorMetabotropic glutamate receptorsIonotropic glutamate receptorsRM-1 cellsCandida albicans SC5314Prostate cancer BALB/C nude mouseMRSA XJ75302-induced sepsis BALB/C mouseantibacterialanticancerantidepressantInhibitorinhibitorinhibit

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