Scoulerine (Synonyms: (-)-Scoulerine; Discretamine)

Name: Scoulerine
Brand: MedChemExpress
SKU: HY-N1255
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1255 Purity: 99.27%: FAQs
Data Sheet SDS COA Handling Instructions

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Scoulerine ((-)-Scoulerine), an isoquinoline alkaloid, is a potent antimitotic compound. Scoulerine is also an inhibitor of BACE1 (?-site amyloid precursor protein cleaving enzyme 1). Scoulerine inhibits proliferation, arrests cell cycle, and induces apoptosis in cancer cells.

For research use only. We do not sell to patients.

Scoulerine Chemical Structure

CAS No. : 6451-73-6

Size	Price	Stock	Quantity
1 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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BACE1 BACE2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

BACE1

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	26.32 μM Compound: (-)-scoulerine	Cytotoxicity against human A549 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
GES1	IC₅₀	95.3 μM Compound: 4	Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HeLa	IC₅₀	6.737 μM Compound: (-)-scoulerine	Cytotoxicity against human HeLa cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
HGC-27	IC₅₀	6.1 μM Compound: 4	Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HRPE	EC₅₀	3.545 μM Compound: (-)-scoulerine	Induction of mitotic arrest in HRPE Cells overexpressing MYC measured for 24 hrs by acridine orange staining based fluorescence microscopic analysis Induction of mitotic arrest in HRPE Cells overexpressing MYC measured for 24 hrs by acridine orange staining based fluorescence microscopic analysis	[PMID: 34406008]
HRPE	EC₅₀	3.595 μM Compound: (-)-scoulerine	Induction of mitotic arrest in HRPE Cells overexpressing MYC assessed as polyploidy measured for 48 hrs by acridine orange staining based fluorescence microscopic analysis Induction of mitotic arrest in HRPE Cells overexpressing MYC assessed as polyploidy measured for 48 hrs by acridine orange staining based fluorescence microscopic analysis	[PMID: 34406008]
HRPE	IC₅₀	4.065 μM Compound: (-)-scoulerine	Cytotoxicity against HRPE cells overexpressing MYC assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against HRPE cells overexpressing MYC assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
HRPE	IC₅₀	4.622 μM Compound: (-)-scoulerine	Cytotoxicity against HRPE cells overexpressing MYC/Bcl2 assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against HRPE cells overexpressing MYC/Bcl2 assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
MDA-MB-231	IC₅₀	7.324 μM Compound: (-)-scoulerine	Cytotoxicity against human MDA-MB-231 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
MGC-803	IC₅₀	12.6 μM Compound: 4	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
NCI-H23	IC₅₀	2.541 μM Compound: (-)-scoulerine	Cytotoxicity against human NCI-H23 cells harboring MYC assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H23 cells harboring MYC assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
SW480	IC₅₀	4.237 μM Compound: (-)-scoulerine	Cytotoxicity against human SW480 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human SW480 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]

In Vitro

Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC₅₀s ranging from 2.7?μM to 6.5?μM^[1].
Scoulerine (2.5-20?μM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells^[1].
Scoulerine (2.5-20?μM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis^[1].
Scoulerine induces G2 or M cell cycle arrest^[1].
Scoulerine (2.5-5?μM; 24?hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells^[1].
Scoulerine (2.5-5?μM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner^[1].
Scoulerine (5-10 μM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells^[1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Jurkat and MOLT-4 cells
Concentration:	2.5, 5, 10, 15 and 20 µM
Incubation Time:	24 hours
Result:	Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner.

Apoptosis Analysis^[1]

Cell Line:	MOLT-4 and Jurkat cells
Concentration:	2.5, 5, 10, 15 and 20 µM
Incubation Time:	24 hours
Result:	Induced MOLT-4 and Jurkat cells apoptosis.

Cell Cycle Analysis^[1]

Cell Line:	Jurkat and MOLT-4 leukemic cells
Concentration:	2.5-20 µM
Incubation Time:	16 hours
Result:	Induced cell cycle arrest at the G2/M transition.

Western Blot Analysis^[1]

Cell Line:	MOLT-4 cells
Concentration:	2.5, 5 µM
Incubation Time:	24 hours
Result:	Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells.

Molecular Weight

327.37

Formula

C₁₉H₂₁NO₄

CAS No.

6451-73-6

Appearance

Solid

Color

Off-white to pink

SMILES

OC1=C(OC)C=C(CCN2CC3=C(O)C(OC)=CC=C3C[C@]24[H])C4=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (305.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0546 mL	15.2732 mL	30.5465 mL
5 mM	0.6109 mL	3.0546 mL	6.1093 mL
10 mM	0.3055 mL	1.5273 mL	3.0546 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.27%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (122 KB) RP-HPLC (132 KB)

Handling Instructions (2659 KB)

References

[1]. Habartova K, et al. Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci Rep. 2018;8(1):4829. Published 2018 Mar 19. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0546 mL	15.2732 mL	30.5465 mL	76.3662 mL
	5 mM	0.6109 mL	3.0546 mL	6.1093 mL	15.2732 mL
	10 mM	0.3055 mL	1.5273 mL	3.0546 mL	7.6366 mL
	15 mM	0.2036 mL	1.0182 mL	2.0364 mL	5.0911 mL
	20 mM	0.1527 mL	0.7637 mL	1.5273 mL	3.8183 mL
	25 mM	0.1222 mL	0.6109 mL	1.2219 mL	3.0546 mL
	30 mM	0.1018 mL	0.5091 mL	1.0182 mL	2.5455 mL
	40 mM	0.0764 mL	0.3818 mL	0.7637 mL	1.9092 mL
	50 mM	0.0611 mL	0.3055 mL	0.6109 mL	1.5273 mL
	60 mM	0.0509 mL	0.2546 mL	0.5091 mL	1.2728 mL
	80 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9546 mL
	100 mM	0.0305 mL	0.1527 mL	0.3055 mL	0.7637 mL

Scoulerine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Scoulerine (Synonyms: (-)-Scoulerine; Discretamine)

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Scoulerine Related Antibodies

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Scoulerine Related Antibodies

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Complete Stock Solution Preparation Table

Scoulerine Related Classifications

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