2. Apoptosis
  3. Apoptosis
  4. Sophoridine

Sophoridine is a quinolizidine alkaloid isolated from Leguminous plant Sophora flavescens. Sophoridine induces apoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor agent candidate for pancreatic cancer with well-tolerated toxicity.

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Sophoridine Chemical Structure

Sophoridine Chemical Structure

CAS No. : 6882-68-4

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10 mM * 1 mL in DMSO
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Based on 4 publication(s) in Google Scholar

Other Forms of Sophoridine:

Sophoridine Related Antibodies

Top Publications Citing Use of Products

    Sophoridine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2021 Feb 24;11:634851.  [Abstract]

    LPS and Sophoridine (20 and 40 μg/mL) triggered the phosphorylation of JNK, ERK, and p38 MAPK in RAW264.7 and THP-1-derived macrophages. RAW264.7 and THP-1 cells are pre-treated with SP600125, and then stimulated with or without Sophoridine (20 and 40 μg/mL).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Sophoridine is a quinolizidine alkaloid isolated from Leguminous plant Sophora flavescens. Sophoridine induces apoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor agent candidate for pancreatic cancer with well-tolerated toxicity[1].

    In Vitro

    Sophoridine (0-500 μM; 48 hours) exhibits remarkable inhibition effects to the growth of human pancreatic, gastric, liver, colon, gallbladder, and prostate carcinoma cells with IC50 values of about 20 μM to 200 μM[1].
    Sophoridine (0-20 μM; 48 hours) increases S phase cell population from 26.23% (control) to 38.67% in Miapaca-2 cells and from 29.56% (control) to 39.16% in PANC-1 cells, about a 1.5-fold and a 1.3-fold increase, respectively[1].
    Sophoridine (0-20 μM; 48 hours) significantly increases bad and bax levels, and decreases bcl-2 and bcl-xl levels in contrast, with a significant increase in Bax/Bcl-2 ratio[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sophoridine Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Normal cells: IOSE144, HL-7702 and LO2, BEAS-2B, GES-1, HEK 293 T, HPDE, FHC, Human cancer cells: PANC-1, Mapaca-1, hepG2, SGC-7901, CBC-SD,SGC-996,PC-3,MKN-45,MGC-803,Hela and HCT116 cells
    Concentration: 0, 3.9, 7.8, 15.5, 31, 62.5, 125, 250, 500 μM
    Incubation Time: 48 hours
    Result: Exhibited the most potent cytotoxicity to cancer cells.

    Cell Cycle Analysis[1]

    Cell Line: PANC-1 cells; Miapca-2 cells
    Concentration: 20 μM
    Incubation Time: 48 hours
    Result: Led to accumulated population in the S phase.

    Western Blot Analysis[1]

    Cell Line: PANC-1 cells; Miapca-2 cells
    Concentration: 20 μM
    Incubation Time: 48 hours
    Result: Induced the activation of intrinsic apoptosis pathway.
    In Vivo

    Sophoridine (intraperitoneal injection; 20 or 40 mg/kg; 21 days) can inhibit the growth of xenograft pancreatic tumors[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c homozygous (nu/nu) nude mice[1]
    Dosage: 20 or 40 mg/kg
    Administration: Intraperitoneal injection; 20 or 40 mg/kg; 21 days
    Result: Decreased xenograft pancreatic tumors mass.
    Molecular Weight

    248.36

    Formula

    C15H24N2O
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1CCC[C@]2([H])[C@@]3([H])CCCN4[C@@]3([H])[C@@](CCC4)([H])CN21
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (201.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.0264 mL 20.1321 mL 40.2641 mL
    5 mM 0.8053 mL 4.0264 mL 8.0528 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.0264 mL 20.1321 mL 40.2641 mL 100.6603 mL
    5 mM 0.8053 mL 4.0264 mL 8.0528 mL 20.1321 mL
    10 mM 0.4026 mL 2.0132 mL 4.0264 mL 10.0660 mL
    15 mM 0.2684 mL 1.3421 mL 2.6843 mL 6.7107 mL
    20 mM 0.2013 mL 1.0066 mL 2.0132 mL 5.0330 mL
    25 mM 0.1611 mL 0.8053 mL 1.6106 mL 4.0264 mL
    30 mM 0.1342 mL 0.6711 mL 1.3421 mL 3.3553 mL
    40 mM 0.1007 mL 0.5033 mL 1.0066 mL 2.5165 mL
    50 mM 0.0805 mL 0.4026 mL 0.8053 mL 2.0132 mL
    60 mM 0.0671 mL 0.3355 mL 0.6711 mL 1.6777 mL
    80 mM 0.0503 mL 0.2517 mL 0.5033 mL 1.2583 mL
    100 mM 0.0403 mL 0.2013 mL 0.4026 mL 1.0066 mL
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    Sophoridine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sophoridine6882-68-4ApoptosisInhibitorinhibitorinhibit

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