Sophoridine

Name: Sophoridine
Brand: MedChemExpress
SKU: HY-N1373
Rating: 5.0 (4 reviews)

Cat. No.: HY-N1373 Purity: 99.45%: FAQs
Data Sheet SDS COA Handling Instructions

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Sophoridine is a quinolizidine alkaloid isolated from Leguminous plant Sophora flavescens. Sophoridine induces apoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor agent candidate for pancreatic cancer with well-tolerated toxicity.

For research use only. We do not sell to patients.

Sophoridine Chemical Structure

CAS No. : 6882-68-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 40	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 40	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 4 publication(s) in Google Scholar

Other Forms of Sophoridine:

d-Sophoridine Get quote
Sophoridine (Standard) Get quote

3 Publications Citing Use of MCE Sophoridine

: Sophoridine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2021 Feb 24;11:634851. [Abstract]; LPS and Sophoridine (20 and 40 μg/mL) triggered the phosphorylation of JNK, ERK, and p38 MAPK in RAW264.7 and THP-1-derived macrophages. RAW264.7 and THP-1 cells are pre-treated with SP600125, and then stimulated with or without Sophoridine (20 and 40 μg/mL).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 80 μM Compound: 1	Antiproliferative activity against human A549 cells after 3 days by SRB assay Antiproliferative activity against human A549 cells after 3 days by SRB assay	[PMID: 26865176]
A549	IC₅₀	5836 μM Compound: Sophoridine	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30025344]
CNE-2	IC₅₀	5379 μM Compound: Sophoridine	Cytotoxicity against human CNE2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human CNE2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30025344]
HeLa	IC₅₀	4332 μM Compound: Sophoridine	Antiproliferative activity against human HeLa cells by MTT assay Antiproliferative activity against human HeLa cells by MTT assay	[PMID: 30007563]
HepG2	IC₅₀	> 20 μg/mL Compound: 1	Antiproliferative activity against human HepG2 cells assessed as growth inhibition after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 24826818]
HepG2	IC₅₀	> 80 μM Compound: 1	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 25408835]
HepG2	IC₅₀	1160 μg/mL Compound: Sophoridine	Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay	[PMID: 26299348]
HepG2	IC₅₀	4509 μM Compound: Sophoridine	Antiproliferative activity against human HepG2 cells by MTT assay Antiproliferative activity against human HepG2 cells by MTT assay	[PMID: 30007563]
HepG2	IC₅₀	4670 μM Compound: Sophoridine	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 30125725]
HepG2	IC₅₀	4670 μM Compound: Sophoridine	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30025344]
KB	IC₅₀	> 80 μM Compound: 1	Antiproliferative activity against human KB cells after 3 days by SRB assay Antiproliferative activity against human KB cells after 3 days by SRB assay	[PMID: 26865176]
MCF7	IC₅₀	> 80 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 26865176]
MDA-MB-231	IC₅₀	> 80 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay	[PMID: 26865176]

In Vitro

Sophoridine (0-500 μM; 48 hours) exhibits remarkable inhibition effects to the growth of human pancreatic, gastric, liver, colon, gallbladder, and prostate carcinoma cells with IC₅₀ values of about 20 μM to 200 μM^[1].
Sophoridine (0-20 μM; 48 hours) increases S phase cell population from 26.23% (control) to 38.67% in Miapaca-2 cells and from 29.56% (control) to 39.16% in PANC-1 cells, about a 1.5-fold and a 1.3-fold increase, respectively^[1].
Sophoridine (0-20 μM; 48 hours) significantly increases bad and bax levels, and decreases bcl-2 and bcl-xl levels in contrast, with a significant increase in Bax/Bcl-2 ratio^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Normal cells: IOSE144, HL-7702 and LO2, BEAS-2B, GES-1, HEK 293 T, HPDE, FHC, Human cancer cells: PANC-1, Mapaca-1, hepG2, SGC-7901, CBC-SD,SGC-996,PC-3,MKN-45,MGC-803,Hela and HCT116 cells
Concentration:	0, 3.9, 7.8, 15.5, 31, 62.5, 125, 250, 500 μM
Incubation Time:	48 hours
Result:	Exhibited the most potent cytotoxicity to cancer cells.

Cell Cycle Analysis^[1]

Cell Line:	PANC-1 cells; Miapca-2 cells
Concentration:	20 μM
Incubation Time:	48 hours
Result:	Led to accumulated population in the S phase.

Western Blot Analysis^[1]

Cell Line:	PANC-1 cells; Miapca-2 cells
Concentration:	20 μM
Incubation Time:	48 hours
Result:	Induced the activation of intrinsic apoptosis pathway.

In Vivo

Sophoridine (intraperitoneal injection; 20 or 40 mg/kg; 21 days) can inhibit the growth of xenograft pancreatic tumors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c homozygous (nu/nu) nude mice^[1]
Dosage:	20 or 40 mg/kg
Administration:	Intraperitoneal injection; 20 or 40 mg/kg; 21 days
Result:	Decreased xenograft pancreatic tumors mass.

Molecular Weight

248.36

Formula

C₁₅H₂₄N₂O

CAS No.

6882-68-4

Appearance

Solid

Color

White to off-white

SMILES

O=C1CCC[C@]2([H])[C@@]3([H])CCCN4[C@@]3([H])[C@@](CCC4)([H])CN21

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (201.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0264 mL	20.1321 mL	40.2641 mL
5 mM	0.8053 mL	4.0264 mL	8.0528 mL
10 mM	0.4026 mL	2.0132 mL	4.0264 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.17 mg/mL (8.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (281 KB) SDS (419 KB)

COA (255 KB) LCMS (89 KB)

Handling Instructions (2659 KB)

References

[1]. Xu Z, et al. Sophoridine induces apoptosis and S phase arrest via ROS-dependent JNK and ERK activation in human pancreatic cancer cells. J Exp Clin Cancer Res. 2017 Sep 11;36(1):124. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0264 mL	20.1321 mL	40.2641 mL	100.6603 mL
	5 mM	0.8053 mL	4.0264 mL	8.0528 mL	20.1321 mL
	10 mM	0.4026 mL	2.0132 mL	4.0264 mL	10.0660 mL
	15 mM	0.2684 mL	1.3421 mL	2.6843 mL	6.7107 mL
	20 mM	0.2013 mL	1.0066 mL	2.0132 mL	5.0330 mL
	25 mM	0.1611 mL	0.8053 mL	1.6106 mL	4.0264 mL
	30 mM	0.1342 mL	0.6711 mL	1.3421 mL	3.3553 mL
	40 mM	0.1007 mL	0.5033 mL	1.0066 mL	2.5165 mL
	50 mM	0.0805 mL	0.4026 mL	0.8053 mL	2.0132 mL
	60 mM	0.0671 mL	0.3355 mL	0.6711 mL	1.6777 mL
	80 mM	0.0503 mL	0.2517 mL	0.5033 mL	1.2583 mL
	100 mM	0.0403 mL	0.2013 mL	0.4026 mL	1.0066 mL

Sophoridine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sophoridine6882-68-4ApoptosisInhibitorinhibitorinhibit

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Sophoridine

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Other Forms of Sophoridine:

Sophoridine Related Antibodies

3 Publications Citing Use of MCE Sophoridine

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•Related Screening Libraries:

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Sophoridine Related Antibodies

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Complete Stock Solution Preparation Table

Sophoridine Related Classifications

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