2. MAPK/ERK Pathway Stem Cell/Wnt Metabolic Enzyme/Protease
  3. MEK ERK Endogenous Metabolite
  4. trans-Zeatin

trans-Zeatin is a plant cytokinin, which plays an important role in cell growth, differentiation, and division; trans-Zeatin also inhibits UV-induced MEK/ERK activation.

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trans-Zeatin Chemical Structure

trans-Zeatin Chemical Structure

CAS No. : 1637-39-4

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of trans-Zeatin:

trans-Zeatin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE trans-Zeatin

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MEK1 MEK2 MEK5 MEK

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

trans-Zeatin is a plant cytokinin, which plays an important role in cell growth, differentiation, and division; trans-Zeatin also inhibits UV-induced MEK/ERK activation.

IC50 & Target[2]

MEK

 

ERK

 

In Vitro

trans-Zeatin is a plant cytokinin, which plays an important role in cell growth, differentiation, and division[1]. trans-Zeatin (20, 40 or 80 μM) inhibits UV-induced MEK/ERK activation, upregulates AQP3 in a time- and dose-dependent manner, and attenuates UV induced loss of AQP3 in keratinocytes (HaCaT cells). UV-induced AQP3 downregulation is blocked by MEK/ERK inhibitors. Trans-Zeatin (80 μM) attenuates UV-induced downregulation of wound healing and water permeability in HaCaT cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

trans-Zeatin Related Antibodies
Molecular Weight

219.24

Formula

C10H13N5O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC/C(C)=C/CNC1=C2N=CNC2=NC=N1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (114.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5612 mL 22.8061 mL 45.6121 mL
5 mM 0.9122 mL 4.5612 mL 9.1224 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (5.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (5.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.83%

COA (244 KB) HNMR (271 KB) RP-HPLC (246 KB) MS (176 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5612 mL 22.8061 mL 45.6121 mL 114.0303 mL
5 mM 0.9122 mL 4.5612 mL 9.1224 mL 22.8061 mL
10 mM 0.4561 mL 2.2806 mL 4.5612 mL 11.4030 mL
15 mM 0.3041 mL 1.5204 mL 3.0408 mL 7.6020 mL
20 mM 0.2281 mL 1.1403 mL 2.2806 mL 5.7015 mL
25 mM 0.1824 mL 0.9122 mL 1.8245 mL 4.5612 mL
30 mM 0.1520 mL 0.7602 mL 1.5204 mL 3.8010 mL
40 mM 0.1140 mL 0.5702 mL 1.1403 mL 2.8508 mL
50 mM 0.0912 mL 0.4561 mL 0.9122 mL 2.2806 mL
60 mM 0.0760 mL 0.3801 mL 0.7602 mL 1.9005 mL
80 mM 0.0570 mL 0.2851 mL 0.5702 mL 1.4254 mL
100 mM 0.0456 mL 0.2281 mL 0.4561 mL 1.1403 mL
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trans-Zeatin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

trans-Zeatin1637-39-4MEKERKEndogenous MetaboliteMitogen-activated protein kinase kinaseMAPKKMAP2KExtracellular signal regulated kinasesInhibitorinhibitorinhibit

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