2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis
  3. Reactive Oxygen Species Apoptosis
  4. Traumatic Acid

Traumatic Acid is a wound healing agent and a cytokinin (phytohormone). Traumatic Acid enhances the biosynthesis of collagen in cultured human skin fibroblasts. Traumatic Acid inhibits MCF-7 breast cancer cells viability and enhances apoptosis and oxidative stress. Traumatic Acid can be used in studies of cancer, circulatory disorders (including arterial hypertension), and skin diseases associated with oxidative stress and impaired collagen biosynthesis.

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Traumatic Acid Chemical Structure

Traumatic Acid Chemical Structure

CAS No. : 6402-36-4

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10 mM * 1 mL in DMSO
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Based on 2 publication(s) in Google Scholar

Traumatic Acid Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

Traumatic Acid is a wound healing agent and a cytokinin (phytohormone). Traumatic Acid enhances the biosynthesis of collagen in cultured human skin fibroblasts. Traumatic Acid inhibits MCF-7 breast cancer cells viability and enhances apoptosis and oxidative stress. Traumatic Acid can be used in studies of cancer, circulatory disorders (including arterial hypertension), and skin diseases associated with oxidative stress and impaired collagen biosynthesis[1][2].

IC50 & Target

IC50: collagen biosynthesis[1]
In Vitro

Traumatic Acid (0.1, 1 µM; 5 days) significantly increases cell number in fibroblasts[1].
Traumatic Acid (0.1, 1 µM; 5 days) increases content of GPX activity and reduced glutathione, as well as decreases membrane phospholipid peroxidation in fibroblasts[1].
Traumatic Acid (0.1, 1 µM; 5 days) enhances the production and secretion of medium collagen in medium of fibroblasts[1].
Traumatic Acid (100, 200, 400, 600 µM; 48 h) significantly decreases live cell number, especially after 48h treatment at 100µM and 200µM in MCF-7 cells[2].
Traumatic Acid (50-600 µM; 24, 48 h) causes dose-and time-dependent reduction in cell viability and induces apoptosis in MCF-7 cells[2].
Traumatic Acid (50-200 µM; 24, 48 h) results in an oxidative damage of protein in MCF-7 cells[2].
Traumatic Acid (100, 200 µM; 24, 48 h) efficiently enhances oxidative stress level in MCF-7 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Traumatic Acid Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Fibroblasts
Concentration: 0.1, 1 µM
Incubation Time: 5 days
Result: Caused a significant increase in cell number, especially on day 1 at a concentration of 1 µM.
Increased cell number of 133 % and 118 % compared to the untreated control cells for concentrations of 1 and 0.1 µM, respectively.

Cell Viability Assay[1]

Cell Line: Fibroblasts
Concentration: 0.1, 1 µM
Incubation Time: 5 days
Result: Increased total protein content of 183 % and 90% compared to the control at concentrations of 1 and 0.1 µM on day 1.
Increased collagen content of 72 % at 0.1 µM (on the day 3) and of 51 % at 1 µM (on the day 1) compared to the control.
Increased GPX activity by 111 % and 97 % at concentrations of 1 and 0.1 µM compared to the control.
Increased content of reduced glutathione of 86 % and 80% at 0.1 and 1 µM, respectively.
Decreased membrane phospholipid peroxidation.

Cell Viability Assay[2]

Cell Line: MCF-7 cells
Concentration: 100, 200, 400, 600 µM
Incubation Time: 48 h
Result: Decreasd live cell number of about 76% at 100 µM concentration.

Cell Viability Assay[2]

Cell Line: MCF-7 cells
Concentration: 50-200 µM
Incubation Time: 24, 48 h
Result: Increased thiol group content of 167% at 100µM and 24 h.

Cell Viability Assay[2]

Cell Line: MCF-7 cells
Concentration: 100, 200 µM
Incubation Time: 24, 48 h
Result: Increased the amount of ROS.

Apoptosis Analysis[2]

Cell Line: MCF-7 cells
Concentration: 50-600 µM
Incubation Time: 24, 48 h
Result: Increased level of apoptosis in a time- and dose-dependent manner.
Molecular Weight

228.28

Formula

C12H20O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)/C=C/CCCCCCCCC(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1095.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3806 mL 21.9029 mL 43.8059 mL
5 mM 0.8761 mL 4.3806 mL 8.7612 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.85%

COA (240 KB) HNMR (258 KB) LCMS (91 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.3806 mL 21.9029 mL 43.8059 mL 109.5146 mL
5 mM 0.8761 mL 4.3806 mL 8.7612 mL 21.9029 mL
10 mM 0.4381 mL 2.1903 mL 4.3806 mL 10.9515 mL
15 mM 0.2920 mL 1.4602 mL 2.9204 mL 7.3010 mL
20 mM 0.2190 mL 1.0951 mL 2.1903 mL 5.4757 mL
25 mM 0.1752 mL 0.8761 mL 1.7522 mL 4.3806 mL
30 mM 0.1460 mL 0.7301 mL 1.4602 mL 3.6505 mL
40 mM 0.1095 mL 0.5476 mL 1.0951 mL 2.7379 mL
50 mM 0.0876 mL 0.4381 mL 0.8761 mL 2.1903 mL
60 mM 0.0730 mL 0.3650 mL 0.7301 mL 1.8252 mL
80 mM 0.0548 mL 0.2738 mL 0.5476 mL 1.3689 mL
100 mM 0.0438 mL 0.2190 mL 0.4381 mL 1.0951 mL
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Traumatic Acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Traumatic Acid6402-36-4Reactive Oxygen SpeciesApoptosiscancerarterial hypertensionfibroblastsMCF-7phytohormoneimpaired collagen biosynthesisbreast cancerROSInhibitorinhibitorinhibit

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