Trifluoperazine dihydrochloride

Name: Trifluoperazine dihydrochloride
Brand: MedChemExpress
SKU: HY-B0532A
Rating: 5.0 (7 reviews)

Cat. No.: HY-B0532A Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

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Trifluoperazine dihydrochloride, an antipsychotic agent, acts by blocking central dopamine receptors. Trifluoperazine dihydrochloride is a potent α1-adrenergic receptor antagonist. Trifluoperazine dihydrochloride is a potent NUPR1 inhibitor exerting anticancer activity. Trifluoperazine dihydrochloride is a calmodulin inhibitor, and also inhibits P-glycoprotein. Trifluoperazine dihydrochloride can be used for the research of schizophrenia. Trifluoperazine dihydrochloride acts as a reversible inhibitor of influenza virus morphogenesis.

For research use only. We do not sell to patients.

Trifluoperazine dihydrochloride Chemical Structure

CAS No. : 440-17-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 41	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 41	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Trifluoperazine dihydrochloride:

Trifluoperazine Get quote
Trifluoperazine dimaleate Get quote
Trifluoperazine (dihydrochloride) (Standard) Get quote

7 Publications Citing Use of MCE Trifluoperazine dihydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
KG-1a	IC₅₀	4.58 μM Compound: TFP	Antiproliferative activity against human KG1a cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human KG1a cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
MCF7	IC₅₀	11.33 μM Compound: TFP	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
MDA-MB-231	IC₅₀	21.58 μM Compound: TFP	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
NCI-H1650	GI₅₀	12.2 μM Compound: Trifluoperazine	Cytotoxicity against human NCI-H1650 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26372073]
Neutrophil	IC₅₀	12.2 μM Compound: TFP	Antiinflammatory activity in fMLP/cytochalasin B stimulated rat neutrophils assessed as inhibition of beta-glucuronidase release preincubated for 10 mins measured 45 mins after fMLP/cytochalasin B challenge Antiinflammatory activity in fMLP/cytochalasin B stimulated rat neutrophils assessed as inhibition of beta-glucuronidase release preincubated for 10 mins measured 45 mins after fMLP/cytochalasin B challenge	[PMID: 12662101]
Neutrophil	IC₅₀	13.2 μM Compound: TFP	Antiinflammatory activity in fMLP/cytochalasin B stimulated rat neutrophils assessed as inhibition of lysozyme release preincubated for 10 mins measured 45 mins after fMLP/cytochalasin B challenge Antiinflammatory activity in fMLP/cytochalasin B stimulated rat neutrophils assessed as inhibition of lysozyme release preincubated for 10 mins measured 45 mins after fMLP/cytochalasin B challenge	[PMID: 12662101]
NSC	IC₅₀	10.1 μM Compound: TFP	Cytotoxicity against human NSC cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human NSC cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29614416]
SK-BR-3	IC₅₀	15.89 μM Compound: TFP	Antiproliferative activity against human SKBR3 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human SKBR3 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
SUM-159-PT	IC₅₀	17.03 μM Compound: TFP	Antiproliferative activity against human SUM159 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human SUM159 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
U-87MG ATCC	EC₅₀	13.5 μM Compound: TFP	Induction of intracellular Ca2+ level in human U87MG cells by Fura-2 AM dye-based assay Induction of intracellular Ca2+ level in human U87MG cells by Fura-2 AM dye-based assay	[PMID: 29614416]
U-87MG ATCC	IC₅₀	9.9 μM Compound: TFP	Cytotoxicity against human U87MG cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human U87MG cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29614416]

In Vitro

Trifluoperazine dihydrochloride is a long-established, widely used 'conventional' antipsychotic agent. Trifluoperazine dihydrochloride has been an extensively studied drug molecule that has been used as a calmodulin inhibitor^[3]^[4].
Trifluoperazine dihydrochloride acts as a reversible inhibitor of influenza virus morphogenesis, but not budding, by disturbing cellular CaM and/or CaM-dependent functions^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

480.42

Formula

C₂₁H₂₆Cl₂F₃N₃S

CAS No.

440-17-5

Appearance

Solid

Color

White to off-white

SMILES

FC(C(C=C1N2CCCN3CCN(C)CC3)=CC=C1SC4=C2C=CC=C4)(F)F.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (104.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (104.08 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0815 mL	10.4076 mL	20.8151 mL
5 mM	0.4163 mL	2.0815 mL	4.1630 mL
10 mM	0.2082 mL	1.0408 mL	2.0815 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (277 KB) SDS (563 KB)

COA (251 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Santofimia-Castaño P, et al. Ligand-based design identifies a potent NUPR1 inhibitor exerting anticancer activity via necroptosis. J Clin Invest. 2019;129(6):2500-2513. Published 2019 Mar 28. [Content Brief]

[2]. Huerta-Bahena J, Villalobos-Molina R, García-Sáinz JA. Trifluoperazine and chlorpromazine antagonize alpha 1- but not alpha2- adrenergic effects. Mol Pharmacol. 1983;23(1):67-70. [Content Brief]

[3]. Marques LO, Lima MS, Soares BG. Trifluoperazine for schizophrenia. Cochrane Database Syst Rev. 2004;2004(1):CD003545. [Content Brief]

[4]. Howland RH. Trifluoperazine: A Sprightly Old Drug. J Psychosoc Nurs Ment Health Serv. 2016;54(1):20-22. [Content Brief]

[5]. Ochiai H, et al. Influence of trifluoperazine on the late stage of influenza virus infection in MDCK cells. Antiviral Res. 1991;15(2):149-160. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.0815 mL	10.4076 mL	20.8151 mL	52.0378 mL
	5 mM	0.4163 mL	2.0815 mL	4.1630 mL	10.4076 mL
	10 mM	0.2082 mL	1.0408 mL	2.0815 mL	5.2038 mL
	15 mM	0.1388 mL	0.6938 mL	1.3877 mL	3.4692 mL
	20 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6019 mL
	25 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0815 mL
	30 mM	0.0694 mL	0.3469 mL	0.6938 mL	1.7346 mL
	40 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL
	50 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
	60 mM	0.0347 mL	0.1735 mL	0.3469 mL	0.8673 mL
	80 mM	0.0260 mL	0.1301 mL	0.2602 mL	0.6505 mL
	100 mM	0.0208 mL	0.1041 mL	0.2082 mL	0.5204 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.