Ubiquitination-IN-1

Name: Ubiquitination-IN-1
Brand: MedChemExpress
SKU: HY-135199
Rating: 4.5 (1 reviews)

Cat. No.: HY-135199 Purity: 99.84%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ubiquitination-IN-1 (compound 24) is a ubiquitination and Cksl-Skp2 protein-protein interaction (IC₅₀=0.17 μM) inhibitor. Ubiquitination-IN-1 increases levels of p27. Ubiquitination-IN-1 has the potential for treatment disease by blocking the degradation of tumor suppressors.

For research use only. We do not sell to patients.

Ubiquitination-IN-1 Chemical Structure

CAS No. : 1819330-15-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 88	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 88	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 365	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	3.06 μM Compound: 22d	Antiproliferative activity against human A549 cells after 6 days Antiproliferative activity against human A549 cells after 6 days	[PMID: 26463131]
A549	IC₅₀	5.91 μM Compound: 22d	Antiproliferative activity against human A549 cells after 2 days Antiproliferative activity against human A549 cells after 2 days	[PMID: 26463131]
HT-1080	GI₅₀	1.32 μM Compound: 22d	Antiproliferative activity against human HT1080 cells after 6 days Antiproliferative activity against human HT1080 cells after 6 days	[PMID: 26463131]
NCI-H1299	IC₅₀	3.9 μM Compound: 22d	Antiproliferative activity against human H1299 cells after 2 days Antiproliferative activity against human H1299 cells after 2 days	[PMID: 26463131]

In Vitro

Ubiquitination-IN-1 inhibits A549 and HT1080 cells with IC₅₀s of 0.91 and 0.4 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

429.41

Formula

C₂₁H₁₄F₃N₃O₂S

CAS No.

1819330-15-8

Appearance

Solid

Color

White to yellow

SMILES

O=S(C1=CC=CC(C(F)(F)F)=C1)(NC2=C3N=CC=CC3=C(C4=CC=CN=C4)C=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (194.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3288 mL	11.6439 mL	23.2878 mL
5 mM	0.4658 mL	2.3288 mL	4.6576 mL
10 mM	0.2329 mL	1.1644 mL	2.3288 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.84 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.84%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (255 KB) HNMR (271 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. David Carroll, et al.Quinoline derivatives useful as ubiquitination inhibitors. WO2016025779A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3288 mL	11.6439 mL	23.2878 mL	58.2194 mL
	5 mM	0.4658 mL	2.3288 mL	4.6576 mL	11.6439 mL
	10 mM	0.2329 mL	1.1644 mL	2.3288 mL	5.8219 mL
	15 mM	0.1553 mL	0.7763 mL	1.5525 mL	3.8813 mL
	20 mM	0.1164 mL	0.5822 mL	1.1644 mL	2.9110 mL
	25 mM	0.0932 mL	0.4658 mL	0.9315 mL	2.3288 mL
	30 mM	0.0776 mL	0.3881 mL	0.7763 mL	1.9406 mL
	40 mM	0.0582 mL	0.2911 mL	0.5822 mL	1.4555 mL
	50 mM	0.0466 mL	0.2329 mL	0.4658 mL	1.1644 mL
	60 mM	0.0388 mL	0.1941 mL	0.3881 mL	0.9703 mL
	80 mM	0.0291 mL	0.1455 mL	0.2911 mL	0.7277 mL
	100 mM	0.0233 mL	0.1164 mL	0.2329 mL	0.5822 mL