(+)-Usnic acid

Name: (+)-Usnic acid
Brand: MedChemExpress
SKU: HY-N0656A
Rating: 5.0 (2 reviews)

Cat. No.: HY-N0656A Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(+)-Usnic acid is isolated from isolated from lichens, binds at the ATP-binding pocket of mTOR, and inhibits mTORC1/2 activity. (+)-Usnic acid inhibits the phosphorylation of mTOR downstream effectors: Akt (Ser473), 4EBP1, S6K, induces autophay, with anti-cancer activity. (+)-Usnic acid possesses antimicrobial activity against a number of planktonic gram-positive bacteria, including Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium.

For research use only. We do not sell to patients.

(+)-Usnic acid Chemical Structure

CAS No. : 7562-61-0

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
1 g	USD 25	In-stock	Estimated Time of Arrival: December 31
5 g	USD 50	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of (+)-Usnic acid:

Usnic acid Get quote
(+)-Usnic acid (Standard) Get quote

2 Publications Citing Use of MCE (+)-Usnic acid

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mTOR mTORC1 mTORC2

Biological Activity
Purity & Documentation
References
Customer Review

Description

(+)-Usnic acid is isolated from isolated from lichens, binds at the ATP-binding pocket of mTOR, and inhibits mTORC1/2 activity. (+)-Usnic acid inhibits the phosphorylation of mTOR downstream effectors: Akt (Ser473), 4EBP1, S6K, induces autophay, with anti-cancer activity^[1]. (+)-Usnic acid possesses antimicrobial activity against a number of planktonic gram-positive bacteria, including Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium^[2].

IC₅₀ & Target^[1]

mTORC1

mTORC2

Cellular Effect

Cell Line	Type	Value	Description	References
3LL cell line	IC₅₀	35.1 μM Compound: (-)-UA, usnic acid	Cytotoxicity against mouse 3LL CRL 1642 cells after 48 hrs by MTT assay Cytotoxicity against mouse 3LL CRL 1642 cells after 48 hrs by MTT assay	[PMID: 18558490]
BT-474	IC₅₀	15.1 μM Compound: 1	Antiproliferative activity against human BT-474 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human BT-474 cells incubated for 72 hrs by MTT assay	[PMID: 28245124]
DU-145	IC₅₀	45.9 μM Compound: (-)-UA, usnic acid	Cytotoxicity against human DU145 ATCC HTB 81 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 ATCC HTB 81 cells after 72 hrs by MTT assay	[PMID: 18558490]
K562	IC₅₀	21.8 μM Compound: (-)-UA, usnic acid	Cytotoxicity against human K562 ATCC CCL 243 cells after 72 hrs by MTT assay Cytotoxicity against human K562 ATCC CCL 243 cells after 72 hrs by MTT assay	[PMID: 18558490]
L1210	IC₅₀	17.4 μM Compound: (-)-UA, usnic acid	Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay	[PMID: 18558490]
MCF7	IC₅₀	11.2 μM Compound: 1	Antiproliferative activity against human MCF7 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells incubated for 72 hrs by MTT assay	[PMID: 28245124]
MCF7	IC₅₀	51.7 μM Compound: (-)-UA, usnic acid	Cytotoxicity against human MCF7 ATCC HTB 22 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 ATCC HTB 22 cells after 72 hrs by MTT assay	[PMID: 18558490]
MDA-MB-231	IC₅₀	13.1 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 28245124]
MDA-MB-231	IC₅₀	13.7 μM Compound: 1	Antimigratory activity in human MDA-MB-231 cells assessed as inhibition of cell migration after 23 hrs by by scratch wound healing assay Antimigratory activity in human MDA-MB-231 cells assessed as inhibition of cell migration after 23 hrs by by scratch wound healing assay	[PMID: 28245124]
MDA-MB-231	IC₅₀	14.2 μM Compound: 1	Anti-invasive activity in human MDA-MB-231 cells after 24 hrs by microscopic analysis Anti-invasive activity in human MDA-MB-231 cells after 24 hrs by microscopic analysis	[PMID: 28245124]
MDA-MB-468	IC₅₀	13.7 μM Compound: 1	Antiproliferative activity against human MDA-MB-468 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells incubated for 72 hrs by MTT assay	[PMID: 28245124]
MDCK	ED₅₀	13.1 μM Compound: (-)-1	Antiviral activity against rimantidine, amantidine-resistant Influenza A virus (A/California/07/09(H1N1)) pdm09 infected in MDCK cells assessed as inhibition of viral replication after 1 hrs by hemagglutination assay Antiviral activity against rimantidine, amantidine-resistant Influenza A virus (A/California/07/09(H1N1)) pdm09 infected in MDCK cells assessed as inhibition of viral replication after 1 hrs by hemagglutination assay	[PMID: 25464881]
MDCK	ED₅₀	14.5 μM Compound: (-)-1	Antiviral activity against Influenza virus A/California/07/09 (H1N1) pdm09 infected in MDCK cells after 48 hrs followed by incubated in chicken erythrocytes for 1 hr by hemagglutinination based end-point dilution method Antiviral activity against Influenza virus A/California/07/09 (H1N1) pdm09 infected in MDCK cells after 48 hrs followed by incubated in chicken erythrocytes for 1 hr by hemagglutinination based end-point dilution method	[PMID: 23099095]
SK-BR-3	IC₅₀	14.4 μM Compound: 1	Antiproliferative activity against human SK-BR-3 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human SK-BR-3 cells incubated for 72 hrs by MTT assay	[PMID: 28245124]
T47D	IC₅₀	15.9 μM Compound: 1	Antiproliferative activity against human T47D cells incubated for 72 hrs by MTT assay Antiproliferative activity against human T47D cells incubated for 72 hrs by MTT assay	[PMID: 28245124]
U-251	IC₅₀	19.7 μM Compound: (-)-UA, usnic acid	Cytotoxicity against human U251 RCB 0641 cells after 72 hrs by MTT assay Cytotoxicity against human U251 RCB 0641 cells after 72 hrs by MTT assay	[PMID: 18558490]

Molecular Weight

344.32

Formula

C₁₈H₁₆O₇

CAS No.

7562-61-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C([C@@]1(C)C(OC2=C(C(C)=O)C(O)=C(C)C(O)=C12)=C3)C(C(C)=O)C3=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 1.92 mg/mL (5.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9043 mL	14.5214 mL	29.0428 mL
5 mM	0.5809 mL	2.9043 mL	5.8085 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.63 mg/mL (1.83 mM); Clear solution

This protocol yields a clear solution of ≥ 0.63 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (280 KB) SDS (392 KB)

COA (259 KB) HNMR (246 KB) RP-HPLC (244 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Ebrahim HY, et al. Usnic Acid Benzylidene Analogues as Potent Mechanistic Target of Rapamycin Inhibitors for the Control of Breast Malignancies. J Nat Prod. 2017 Apr 28;80(4):932-952. [Content Brief]

[2]. Francolini I, et al. Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces. Antimicrob Agents Chemother. 2004;48(11):4360-4365. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9043 mL	14.5214 mL	29.0428 mL	72.6069 mL
DMSO	5 mM	0.5809 mL	2.9043 mL	5.8086 mL	14.5214 mL

(+)-Usnic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(+)-Usnic acid7562-61-0mTORBacterialAutophagyMammalian target of RapamycinInhibitorinhibitorinhibit

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(+)-Usnic acid

Customer Review

Other Forms of (+)-Usnic acid:

(+)-Usnic acid Related Antibodies

2 Publications Citing Use of MCE (+)-Usnic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All mTOR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(+)-Usnic acid Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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