Wnt/β-catenin agonist 3 hydrochloride

Name: Wnt/β-catenin agonist 3 hydrochloride
Brand: MedChemExpress
SKU: HY-148055A
Rating: 4.5 (1 reviews)

Cat. No.: HY-148055A Purity: 97.02%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Wnt/β-catenin agonist 3 hydrochloride is a Wnt/β-catenin signalling pathway agonist. Wnt/β-catenin agonist 3 (compound 98) can be used for the research of osteoporosis.

For research use only. We do not sell to patients.

Wnt/β-catenin agonist 3 hydrochloride Chemical Structure

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Wnt/β-catenin agonist 3 hydrochloride:

Wnt/β-catenin agonist 3 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Wnt/β-catenin agonist 3 hydrochloride is a Wnt/β-catenin signalling pathway agonist. Wnt/β-catenin agonist 3 (compound 98) can be used for the research of osteoporosis^[1].

In Vitro

Wnt/β-catenin agonist 3 hydrochloride (compound 98; 24 h; HEK293 and SW480 cells) has 54% cell activity at 120 μM concentration compared with the positive control group LiCl (20mM)^[1].
Wnt/β-catenin agonist 3 hydrochloride (30 and 60 μM; 24 h; HEK293 cells) is an activator for β-Catenin and deposits β-catenin^[1].
Wnt/β-catenin agonist 3 hydrochloride (11 μM; 4 d) induces differentiation of ST2 cell line into osteoblasts and calcium deposition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

367.23

Formula

C₁₆H₁₆Cl₂N₄O₂

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NOC(C2=CC=C(Cl)C=C2)=C1)NCCCN3C=CN=C3.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (136.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7231 mL	13.6154 mL	27.2309 mL
5 mM	0.5446 mL	2.7231 mL	5.4462 mL
10 mM	0.2723 mL	1.3615 mL	2.7231 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.40 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.40 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (3.40 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.02%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (247 KB) HNMR (269 KB) RP-HPLC (287 KB) LCMS (313 KB)

Handling Instructions (2659 KB)

References

[1]. Cho JW, et al. Isoxazole derivatives and use thereof. WO2007078113A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7231 mL	13.6154 mL	27.2309 mL	68.0772 mL
	5 mM	0.5446 mL	2.7231 mL	5.4462 mL	13.6154 mL
	10 mM	0.2723 mL	1.3615 mL	2.7231 mL	6.8077 mL
	15 mM	0.1815 mL	0.9077 mL	1.8154 mL	4.5385 mL
	20 mM	0.1362 mL	0.6808 mL	1.3615 mL	3.4039 mL
	25 mM	0.1089 mL	0.5446 mL	1.0892 mL	2.7231 mL
	30 mM	0.0908 mL	0.4538 mL	0.9077 mL	2.2692 mL
	40 mM	0.0681 mL	0.3404 mL	0.6808 mL	1.7019 mL
	50 mM	0.0545 mL	0.2723 mL	0.5446 mL	1.3615 mL
	60 mM	0.0454 mL	0.2269 mL	0.4538 mL	1.1346 mL
	80 mM	0.0340 mL	0.1702 mL	0.3404 mL	0.8510 mL
	100 mM	0.0272 mL	0.1362 mL	0.2723 mL	0.6808 mL