2. Cell Cycle/DNA Damage
  3. Eukaryotic Initiation Factor (eIF)
  4. (Z)-4EGI-1

(Z)-4EGI-1 is the Z-isomer of 4EGI-1 and is an inhibitor of eIF4E/eIF4G interaction and of translation initiation. (Z)-4EGI-1 effectively binds to eIF4E with an IC50 of 43.5 μM and a Kd value of 8.74 μM. (Z)-4EGI-1 has anticancer activity.

For research use only. We do not sell to patients.

(Z)-4EGI-1 Chemical Structure

(Z)-4EGI-1 Chemical Structure

CAS No. : 901787-88-0

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (Z)-4EGI-1:

(Z)-4EGI-1 Related Antibodies

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Description

(Z)-4EGI-1 is the Z-isomer of 4EGI-1 and is an inhibitor of eIF4E/eIF4G interaction and of translation initiation. (Z)-4EGI-1 effectively binds to eIF4E with an IC50 of 43.5 μM and a Kd value of 8.74 μM. (Z)-4EGI-1 has anticancer activity[1][2].

IC50 & Target

eIF4

 

In Vitro

(Z)-4EGI-1 (15-30 µM; 6 hours; CRL-2813 melanoma cells) treatment markedly reduces the expressions of the regulatory proteins: cyclin D1, cyclin E, and Survivin, while the expressions of housekeeping proteins such as β-Actin and α-Tubulin are not affected[1].
(Z)-4EGI-1 inhibits cancer cells proliferation with IC50 values of 15.3 μM and 11.6 μM for CRL-2351 breast cells and CRL-2813 melanoma cells, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(Z)-4EGI-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: CRL-2813 melanoma cells
Concentration: 15 µM, 30 µM
Incubation Time: 6 hours
Result: Markedly reduced the expressions of the regulatory proteins: cyclin D1, cyclin E, and Survivin.
Molecular Weight

451.28

Formula

C18H12Cl2N4O4S
CAS No.
Appearance

Solid

Color

Light brown to gray

SMILES

O=C(O)/C(CC1=CC=CC=C1[N+]([O-])=O)=N/NC2=NC(C3=CC=C(Cl)C(Cl)=C3)=CS2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (110.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2159 mL 11.0796 mL 22.1592 mL
5 mM 0.4432 mL 2.2159 mL 4.4318 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2159 mL 11.0796 mL 22.1592 mL 55.3980 mL
5 mM 0.4432 mL 2.2159 mL 4.4318 mL 11.0796 mL
10 mM 0.2216 mL 1.1080 mL 2.2159 mL 5.5398 mL
15 mM 0.1477 mL 0.7386 mL 1.4773 mL 3.6932 mL
20 mM 0.1108 mL 0.5540 mL 1.1080 mL 2.7699 mL
25 mM 0.0886 mL 0.4432 mL 0.8864 mL 2.2159 mL
30 mM 0.0739 mL 0.3693 mL 0.7386 mL 1.8466 mL
40 mM 0.0554 mL 0.2770 mL 0.5540 mL 1.3849 mL
50 mM 0.0443 mL 0.2216 mL 0.4432 mL 1.1080 mL
60 mM 0.0369 mL 0.1847 mL 0.3693 mL 0.9233 mL
80 mM 0.0277 mL 0.1385 mL 0.2770 mL 0.6925 mL
100 mM 0.0222 mL 0.1108 mL 0.2216 mL 0.5540 mL
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(Z)-4EGI-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(Z)-4EGI-1901787-88-0Eukaryotic Initiation Factor (eIF)Protein-proteininteractioneIF4EeIF4GtranslationZ-isomerhydrophobicitycyclin D1cyclin ESurvivinanti-proliferationInhibitorinhibitorinhibit

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