ZYZ-488

Name: ZYZ-488
Brand: MedChemExpress
SKU: HY-100472
Rating: 5.0 (1 reviews)

Cat. No.: HY-100472 Purity: 98.69%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ZYZ-488 is a competitive apoptotic protease activating factor-1 (Apaf-1) inhibitor. ZYZ-488 inhibits the activation of binding protein procaspase-9 and procaspase-3.

For research use only. We do not sell to patients.

ZYZ-488 Chemical Structure

CAS No. : 1470302-79-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 385	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 385	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

ZYZ-488 is a competitive apoptotic protease activating factor-1 (Apaf-1) inhibitor. ZYZ-488 inhibits the activation of binding protein procaspase-9 and procaspase-3^[1].

IC₅₀ & Target

Apoptotic protease activating factor-1^[1]

In Vitro

ZYZ-488 decreases apoptosis in hypoxic H9c2 cells. ZYZ-488 inhibits hypoxia induced Apaf-1-mediated activation of procaspase-9 and procaspase-3^[1].
ZYZ-488 decreases myocardial enzyme release, inhibits cardiomyocyte apoptosis, and suppresses the activation of the downstream cascade of caspases^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ZYZ-488 (33.9-67.8 mg/kg; intramuscularly; for 3 days) protected against myocardial apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice (8-10 weeks old)^[2]
Dosage:	myocardial infarction + ZYZ-488 (33.9 mg/kg); myocardial infraction + ZYZ-488 (67.8 mg/kg);
Administration:	Intramuscularly; for 3 days
Result:	Inhibited Apaf-1-mediated activation of procaspase-9 and procaspase-3.

Molecular Weight

487.46

Formula

C₂₀H₂₉N₃O₁₁

CAS No.

1470302-79-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCCCNC(N)=N)C(C=C1OC)=CC(OC)=C1O[C@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](C(O)=O)O2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (410.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (205.15 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0515 mL	10.2573 mL	20.5145 mL
5 mM	0.4103 mL	2.0515 mL	4.1029 mL
10 mM	0.2051 mL	1.0257 mL	2.0515 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.26 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (10.26 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (10.26 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (247 KB) HNMR (267 KB) LCMS (123 KB)

Handling Instructions (2659 KB)

References

[1]. Wang Y, et al. The discovery of a novel inhibitor of apoptotic protease activating factor-1 (Apaf-1) for ischemic heart: synthesis, activity and target identification. Sci Rep. 2016 Jul 22;6:29820. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0515 mL	10.2573 mL	20.5145 mL	51.2863 mL
	5 mM	0.4103 mL	2.0515 mL	4.1029 mL	10.2573 mL
	10 mM	0.2051 mL	1.0257 mL	2.0515 mL	5.1286 mL
	15 mM	0.1368 mL	0.6838 mL	1.3676 mL	3.4191 mL
	20 mM	0.1026 mL	0.5129 mL	1.0257 mL	2.5643 mL
	25 mM	0.0821 mL	0.4103 mL	0.8206 mL	2.0515 mL
	30 mM	0.0684 mL	0.3419 mL	0.6838 mL	1.7095 mL
	40 mM	0.0513 mL	0.2564 mL	0.5129 mL	1.2822 mL
	50 mM	0.0410 mL	0.2051 mL	0.4103 mL	1.0257 mL
	60 mM	0.0342 mL	0.1710 mL	0.3419 mL	0.8548 mL
	80 mM	0.0256 mL	0.1282 mL	0.2564 mL	0.6411 mL
	100 mM	0.0205 mL	0.1026 mL	0.2051 mL	0.5129 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

ZYZ-488 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZYZ-4881470302-79-4ZYZ488ZYZ 488Caspasehypoxiaprocaspase-9procaspase-3myocardialcardiomyocyteInhibitorinhibitorinhibit

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ZYZ-488

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ZYZ-488 Related Antibodies

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ZYZ-488 Related Antibodies

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Complete Stock Solution Preparation Table

ZYZ-488 Related Classifications

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