β-catenin-IN-3

Name: β-catenin-IN-3
Brand: MedChemExpress
SKU: HY-147007
Rating: 4.5 (1 reviews)

Cat. No.: HY-147007 Purity: 98.03%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

β-catenin-IN-3 (compound C2) is a potent and selective β-catenin inhibitor with a K_D value of 54.96 nM. β-catenin-IN-3 acts by targeting a cryptic allosteric modulation site of β-catenin. β-catenin-IN-3 can significantly reduce viability of β-catenin-driven cancer cells.

For research use only. We do not sell to patients.

β-catenin-IN-3 Chemical Structure

CAS No. : 1005288-15-2

Size	Price	Stock	Quantity
1 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 840	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1680	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

IC₅₀ & Target

K_D: 54.96 nM (β-catenin)^[1]

Molecular Weight

484.25

Formula

C₁₉H₂₀Br₂N₂OS

CAS No.

1005288-15-2

Appearance

Solid

Color

White to off-white

SMILES

S=C(N/C=C/C(C=C1Br)=C(C(Br)=C1)O)NC(C2)C3C(C=CC4)C4C2C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (206.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0650 mL	10.3252 mL	20.6505 mL
5 mM	0.4130 mL	2.0650 mL	4.1301 mL
10 mM	0.2065 mL	1.0325 mL	2.0650 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.03%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (247 KB) HNMR (229 KB) LCMS (114 KB)

Handling Instructions (2659 KB)

References

[1]. Cheltsov A, et al. Allosteric inhibitor of β-catenin selectively targets oncogenic Wnt signaling in colon cancer. Sci Rep. 2020;10(1):8096. Published 2020 May 15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0650 mL	10.3252 mL	20.6505 mL	51.6262 mL
	5 mM	0.4130 mL	2.0650 mL	4.1301 mL	10.3252 mL
	10 mM	0.2065 mL	1.0325 mL	2.0650 mL	5.1626 mL
	15 mM	0.1377 mL	0.6883 mL	1.3767 mL	3.4417 mL
	20 mM	0.1033 mL	0.5163 mL	1.0325 mL	2.5813 mL
	25 mM	0.0826 mL	0.4130 mL	0.8260 mL	2.0650 mL
	30 mM	0.0688 mL	0.3442 mL	0.6883 mL	1.7209 mL
	40 mM	0.0516 mL	0.2581 mL	0.5163 mL	1.2907 mL
	50 mM	0.0413 mL	0.2065 mL	0.4130 mL	1.0325 mL
	60 mM	0.0344 mL	0.1721 mL	0.3442 mL	0.8604 mL
	80 mM	0.0258 mL	0.1291 mL	0.2581 mL	0.6453 mL
	100 mM	0.0207 mL	0.1033 mL	0.2065 mL	0.5163 mL

β-catenin-IN-3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-catenin-IN-31005288-15-2β-cateninWntBeta cateninAnticancerWnt pathwayβ-catenin-driven cancer cellsInhibitorinhibitorinhibit

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β-catenin-IN-3

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References

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Molarity Calculator

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Complete Stock Solution Preparation Table

β-catenin-IN-3 Related Classifications

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