β-Naphthoflavone (Synonyms: 5,6-Benzoflavone; beta-NF)

Name: β-Naphthoflavone
Brand: MedChemExpress
SKU: HY-114740
Rating: 4.5 (1 reviews)

Cat. No.: HY-114740 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

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β-Naphthoflavone is an exogenous aryl hydrocarbon receptor (AHR) ligand. β-Naphthoflavone can activate AHR to participate in various biological processes, including cell growth, differentiation, apoptosis, and metabolism. β-Naphthoflavone has antioxidant activity and can exert its antioxidant function by regulating the activity of antioxidant enzymes. β-Naphthoflavone is also a non-carcinogenic CYP1A inducer and can be used to treat aristolochic acid (AAI) induced renal injury.

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β-Naphthoflavone Chemical Structure

CAS No. : 6051-87-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE β-Naphthoflavone

•Phytomedicine. 2023 Mar 24;114:154774. [Abstract]

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	EC₅₀	8 μM Compound: beta-naphthoflavone	Induction of ARE in human HepG2 cells by luciferase assay Induction of ARE in human HepG2 cells by luciferase assay	[PMID: 16562850]
MCF7	EC₅₀	1.4 μM Compound: 1a	Agonist activity at aryl hydrocarbon receptor in human MCF7 cells after 24 hrs CYP1A1-dependent EROD assay Agonist activity at aryl hydrocarbon receptor in human MCF7 cells after 24 hrs CYP1A1-dependent EROD assay	[PMID: 20060304]
MDCK-II	IC₅₀	6.05 μM Compound: 26	Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of pheophorbide-A preincubated for 30 mins before pheophorbide-A addition measured after 120 mins by flow cytometry Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of pheophorbide-A preincubated for 30 mins before pheophorbide-A addition measured after 120 mins by flow cytometry	[PMID: 23851114]
MDCK-II	IC₅₀	8.32 μM Compound: 26	Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of Hoechst 33342 preincubated for 30 mins before Hoechst 33342 addition measured after 120 mins by fluorescence assay Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of Hoechst 33342 preincubated for 30 mins before Hoechst 33342 addition measured after 120 mins by fluorescence assay	[PMID: 23851114]

In Vitro

β-Naphthoflavone (0.1, 1 and 10 µM; 3 days) increases CYP1A1 mRNA levels in HepG2 cells in a dose-dependent manner and decreases the level of labile zinc in HepG2 cells by approximately 5%, 10% and 17%, respectively^[1].
β-Naphthoflavone (0-50 µM; 24 h) attenuats the decreased viability and increases apoptosis of neuronal SH-SY5Y cells induced by H₂O₂^[2].
β-Naphthoflavone (10 µM) significantly increases the activities of CAT and SOD enzymes in neuronal SH-SY5Y cells and inhibites p38MAPK to attenuate H₂O₂ induced damage^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	human neuron SH‑SY5Y cells
Concentration:	0-50 µM
Incubation Time:	24 h
Result:	Restored SH‑SY5Y cell viability from 0-10 µM, followed by a slight decrease.

Apoptosis Analysis^[2]

Cell Line:	PC cells
Concentration:	10 µM
Incubation Time:	15 min
Result:	Reduced the apoptosis rate of PC cells.

Western Blot Analysis^[2]

Cell Line:	human neuron SH‑SY5Y cells
Concentration:	10 µM
Incubation Time:	24 h
Result:	Reduced the expression of Bax and Cyt-C and the cleavage rate of caspase-3.

In Vivo

β-Naphthoflavone (80 mg/kg, i.p.; once daily for 3 days) protects mice from AAI-induced renal injury and reduces the accumulation of AAI in the mouse kidneys^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice ^[3]
Dosage:	80 mg/kg
Administration:	Intraperitoneal injection (i.p.), once a day for 3 days
Result:	Prevented AAI-induced tubulointerstitial fibrosis by inhibiting the expression of α-SMA, a marker of myofibroblast phenotype induced by AAI. Reduced the formation of AAI metabolite ALI in liver and kidney and induced the expression of CYP1A1 and CYP1A2 in liver and only CYP1A1 in kidney.

Molecular Weight

272.30

Formula

C₁₉H₁₂O₂

CAS No.

6051-87-2

Appearance

Solid

Color

White to light yellow

SMILES

O=C1C2=C3C=CC=CC3=CC=C2OC(C4=CC=CC=C4)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (91.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6724 mL	18.3621 mL	36.7242 mL
5 mM	0.7345 mL	3.6724 mL	7.3448 mL
10 mM	0.3672 mL	1.8362 mL	3.6724 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1 mg/mL (3.67 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (3.67 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (253 KB) HNMR (258 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Ishida T, Takechi S. β-Naphthoflavone, an exogenous ligand of aryl hydrocarbon receptor, disrupts zinc homeostasis in human hepatoma HepG2 cells. J Toxicol Sci. 2019;44(10):711-720. [Content Brief]

[2]. Zhu Y, et al. α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage. Exp Ther Med. 2017 Mar;13(3):1143-1150. [Content Brief]

[3]. Ying Xiao, et al. β-Naphthoflavone protects mice from aristolochic acid-I-induced acute kidney injury in a CYP1A dependent mechanism. Acta Pharmacologica Sinica 30.11 (2009): 1559-1565. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6724 mL	18.3621 mL	36.7242 mL	91.8105 mL
	5 mM	0.7345 mL	3.6724 mL	7.3448 mL	18.3621 mL
	10 mM	0.3672 mL	1.8362 mL	3.6724 mL	9.1811 mL
	15 mM	0.2448 mL	1.2241 mL	2.4483 mL	6.1207 mL
	20 mM	0.1836 mL	0.9181 mL	1.8362 mL	4.5905 mL
	25 mM	0.1469 mL	0.7345 mL	1.4690 mL	3.6724 mL
	30 mM	0.1224 mL	0.6121 mL	1.2241 mL	3.0604 mL
	40 mM	0.0918 mL	0.4591 mL	0.9181 mL	2.2953 mL
	50 mM	0.0734 mL	0.3672 mL	0.7345 mL	1.8362 mL
	60 mM	0.0612 mL	0.3060 mL	0.6121 mL	1.5302 mL
	80 mM	0.0459 mL	0.2295 mL	0.4591 mL	1.1476 mL

β-Naphthoflavone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Naphthoflavone6051-87-25,6-Benzoflavone beta-NFAryl Hydrocarbon ReceptorApoptosisAhRInhibitorinhibitorinhibit

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β-Naphthoflavone (Synonyms: 5,6-Benzoflavone; beta-NF)

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β-Naphthoflavone Related Antibodies

1 Publications Citing Use of MCE β-Naphthoflavone

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References

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β-Naphthoflavone Related Antibodies

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Complete Stock Solution Preparation Table

β-Naphthoflavone Related Classifications

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