(2R,3R)-Butane-2,3-diol

Name: (2R,3R)-Butane-2,3-diol
Brand: MedChemExpress
SKU: HY-W015954
Rating: 4.5 (1 reviews)

Cat. No.: HY-W015954 Purity: 99.17%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

(2R,3R)-Butane-2,3-diol is a non-covalent, reversible agonist targeting lanthanum (La³⁺)-sensitive calcium channels in bacteria (e.g., Escherichia coli) with an EC₅₀ of approximately 25 mM. (2R,3R)-Butane-2,3-diol binds to calcium channel proteins or related complexes, induces channel opening, promotes extracellular calcium influx, and triggers intracellular calcium transients, which may regulate bacterial physiological activities such as growth, metabolism, and signal transduction. (2R,3R)-Butane-2,3-diol mediates bacterial-host cell signaling interactions and affects the metabolic balance of intestinal microorganisms, and can be used to study lactose intolerance and other related diseases.

For research use only. We do not sell to patients.

(2R,3R)-Butane-2,3-diol Chemical Structure

CAS No. : 24347-58-8

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 52	In-stock	Estimated Time of Arrival: May 14
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: May 14
Liquid
1 g	USD 50	In-stock	Estimated Time of Arrival: May 14
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10 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (2R,3R)-Butane-2,3-diol:

(2R,3R)-Butane-2,3-diol (Standard) Get quote

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

La³⁺-sensitive calcium channel

25 mM (EC₅₀)

In Vitro

(2R,3R)-Butane-2,3-diol (100 mM; 1-2 min) significantly induces a rapid increase in intracellular free Ca²⁺ concentration in the Escherichia coli intracellular calcium transient assay, with the peak value increasing by about 1-2 μM compared with the control group, and this effect is dependent on the external Ca²⁺ concentration (0.1-10 mM)^[1].
(2R,3R)-Butane-2,3-diol (5-200 mM; 5 min) activates the E. coli Ca²⁺ channel in a dose-dependent manner in the concentration-dependent assay, with EC₅₀ of about 25 mM. Under the condition of 10 mM external Ca²⁺, the peak intracellular Ca²⁺ could reach 25 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

90.12

Formula

C₄H₁₀O₂

CAS No.

24347-58-8

Appearance

Liquid (Density: 0.992 g/cm³)

Color

Colorless to light yellow

SMILES

C[C@@H](O)[C@H](O)C

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (1109.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	11.0963 mL	55.4816 mL	110.9632 mL
5 mM	2.2193 mL	11.0963 mL	22.1926 mL
10 mM	1.1096 mL	5.5482 mL	11.0963 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (27.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (27.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.72 mg/mL (19.09 mM); Clear solution

This protocol yields a clear solution of ≥ 1.72 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (17.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 99.17%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (251 KB) HNMR (115 KB) GC (265 KB)

Handling Instructions (2659 KB)

References

[1]. Campbell AK, et al. Fermentation product butane 2,3-diol induces Ca2+ transients in E. coli through activation of lanthanum-sensitive Ca2+ channels. Cell Calcium. 2007 Feb;41(2):97-106. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	11.0963 mL	55.4816 mL	110.9632 mL	277.4079 mL
	5 mM	2.2193 mL	11.0963 mL	22.1926 mL	55.4816 mL
	10 mM	1.1096 mL	5.5482 mL	11.0963 mL	27.7408 mL
	15 mM	0.7398 mL	3.6988 mL	7.3975 mL	18.4939 mL
	20 mM	0.5548 mL	2.7741 mL	5.5482 mL	13.8704 mL
	25 mM	0.4439 mL	2.2193 mL	4.4385 mL	11.0963 mL
	30 mM	0.3699 mL	1.8494 mL	3.6988 mL	9.2469 mL
	40 mM	0.2774 mL	1.3870 mL	2.7741 mL	6.9352 mL
	50 mM	0.2219 mL	1.1096 mL	2.2193 mL	5.5482 mL
	60 mM	0.1849 mL	0.9247 mL	1.8494 mL	4.6235 mL
	80 mM	0.1387 mL	0.6935 mL	1.3870 mL	3.4676 mL
	100 mM	0.1110 mL	0.5548 mL	1.1096 mL	2.7741 mL