5,7,4'-Trimethoxyflavone

Name: 5,7,4'-Trimethoxyflavone
Brand: MedChemExpress
SKU: HY-N6818
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6818 Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5,7,4’-Trimethoxyflavone can be isolated from the medicinal plant Kaempferia parviflora (KP). 5,7,4’-Trimethoxyflavone is a CFTR activator and EC₅₀ is 64 μM. 5,7,4’-Trimethoxyflavone induces apoptosis, increases proteolytic activation of caspase-3, and degradation of ADP-ribose polymerase (PARP) protein. 5,7,4’-Trimethoxyflavone has antitumor activity. 5,7,4’-Trimethoxyflavone can be used to prevent skin aging and oxidative stress.

For research use only. We do not sell to patients.

5,7,4'-Trimethoxyflavone Chemical Structure

CAS No. : 5631-70-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5,7,4'-Trimethoxyflavone:

5,7,4'-Trimethoxyflavone (Standard) Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All PARP Isoform Specific Products:

View All Isoforms

PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Caspase-3

PARP

In Vitro

5,7,4’-Trimethoxyflavone (12.5, 25, 50, 100, 200 μM, 24, 48 h) inhibits cell proliferation in SUN-16 cells^[1].
5,7,4’-Trimethoxyflavone (12.5, 25, 37.5, 50 μM, 24 h) induces apoptosis in SUN-16 cells through endoplasmic reticulum stress-regulated proteins^[1].
5,7,4’-Trimethoxyflavone (6.25, 12.5 μM, 24 h) inhibits the activity of TNF-α induced ROS and pro-inflammatory regulators in HDFs cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	AGS, SNU-1, SNU-16
Concentration:	12.5 ,25, 50, 100, 200 μM
Incubation Time:	24 and 48 h
Result:	Showed the highest cytotoxicity in SNU-16 cells.

Apoptosis Analysis^[1]

Cell Line:	SNU-16
Concentration:	12.5 ,25, 37.5, 50 μM
Incubation Time:	24 h
Result:	Increased the percentage of annexin V-positive SNU-16 cells from 7.2% to 58.0% and the expression of GRP78、IRE1a、ATF-4 and CHOP.

Western Blot Analysis^[2]

Cell Line:	HDFs
Concentration:	6.25, 12.5 μM
Incubation Time:	24 h
Result:	Inhibited matrix metalloproteinase-1 ( MMP-1) expression and stimulated collagen, type I, and alpha 1 (COLIA1) expression.

Molecular Weight

312.32

Formula

C₁₈H₁₆O₅

CAS No.

5631-70-9

Appearance

Solid

Color

White to yellow

SMILES

COC1=C(C(C=C(C2=CC=C(OC)C=C2)O3)=O)C3=CC(OC)=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (320.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2018 mL	16.0092 mL	32.0184 mL
5 mM	0.6404 mL	3.2018 mL	6.4037 mL
10 mM	0.3202 mL	1.6009 mL	3.2018 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.65%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (248 KB) HNMR (198 KB) LCMS (133 KB)

Handling Instructions (2659 KB)

References

[1]. Kim H, et al. Induction of ER Stress-Mediated Apoptosis by the Major Component 5,7,4'-TrimethoxyflavoneIsolated from Kaempferia parviflora Tea Infusion. Nutr Cancer. 2018 Aug-Sep;70(6):984-996. [Content Brief]

[2]. Phung HM,et al. Protective Effect of Polymethoxyflavones Isolated from Kaempferia parviflora against TNF-α-Induced Human Dermal Fibroblast Damage. Antioxidants (Basel). 2021 Oct 13;10(10):1609 [Content Brief]

[3]. Fischer H, et al. Activation of the CFTR Cl- channel by trimethoxyflavone in vitro and in vivo. Cell Physiol Biochem. 2008;22(5-6):685-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2018 mL	16.0092 mL	32.0184 mL	80.0461 mL
	5 mM	0.6404 mL	3.2018 mL	6.4037 mL	16.0092 mL
	10 mM	0.3202 mL	1.6009 mL	3.2018 mL	8.0046 mL
	15 mM	0.2135 mL	1.0673 mL	2.1346 mL	5.3364 mL
	20 mM	0.1601 mL	0.8005 mL	1.6009 mL	4.0023 mL
	25 mM	0.1281 mL	0.6404 mL	1.2807 mL	3.2018 mL
	30 mM	0.1067 mL	0.5336 mL	1.0673 mL	2.6682 mL
	40 mM	0.0800 mL	0.4002 mL	0.8005 mL	2.0012 mL
	50 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6009 mL
	60 mM	0.0534 mL	0.2668 mL	0.5336 mL	1.3341 mL
	80 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0006 mL
	100 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL