7β-Hydroxycholesterol

Name: 7β-Hydroxycholesterol
Brand: MedChemExpress
SKU: HY-113341
Rating: 4.5 (1 reviews)

Cat. No.: HY-113341 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

7β-Hydroxycholesterol is an oxysterol that derived by the oxidation of cholesterol. 7β-hydroxycholesterol is a powerful inducer of oxidative stress, inducing dysfunction of organelles (mitochondria, lysosomes and peroxisomes) that can cause cell death.

For research use only. We do not sell to patients.

7β-Hydroxycholesterol Chemical Structure

CAS No. : 566-27-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 7β-Hydroxycholesterol:

7ß-Hydroxycholesterol-d₇ Get quote

1 Publications Citing Use of MCE 7β-Hydroxycholesterol

•Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	13.6 μM Compound: 6	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
A549	IC₅₀	13.6 μM Compound: 8	Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
ARPE-19	IC₅₀	21.6 μM Compound: 6	Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
ARPE-19	IC₅₀	21.6 μM Compound: 8	Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
BJ	IC₅₀	20 μM Compound: 8	Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
BJ	IC₅₀	20.1 μM Compound: 6	Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
BJ	IC₅₀	20.1 μM Compound: 3	Cytotoxicity against human BJ cells after 48 hrs by Alamar Blue assay Cytotoxicity against human BJ cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
C6	IC₅₀	15 μM Compound: 7beta-OHC	Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method	[PMID: 24211631]
HEK293	EC₅₀	10 μM Compound: 5	Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis	[PMID: 23830695]
HEK293	EC₅₀	3.4 μM Compound: 10	Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy	[PMID: 24928400]
HepG2	IC₅₀	14.6 μM Compound: 6	Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HepG2	IC₅₀	14.6 μM Compound: 8	Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HMEC-1	IC₅₀	19.1 μM Compound: 8	Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HT-29	IC₅₀	6.4 μM Compound: 3	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
HT-29	IC₅₀	6.9 μM Compound: 6	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HT-29	IC₅₀	6.9 μM Compound: 8	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HT-29	IC₅₀	7.2 μM Compound: 8	Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay	[PMID: 20931970]
LAMA-84	IC₅₀	4.3 μM Compound: 6	Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
LAMA-84	IC₅₀	4.3 μM Compound: 8	Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
LAMA-84	IC₅₀	4.8 μM Compound: 3	Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar Blue assay Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
MCF7	IC₅₀	8.7 μM Compound: 6	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
MCF7	IC₅₀	8.7 μM Compound: 8	Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
PC-3	IC₅₀	21.9 μM Compound: 8	Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
PC-3	IC₅₀	22 μM Compound: 6	Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
SH-SY5Y	IC₅₀	16.4 μM Compound: 6	Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]

Molecular Weight

402.65

Formula

C₂₇H₄₆O₂

CAS No.

566-27-8

Appearance

Solid

Color

White to off-white

SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])[C@@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (248.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4835 mL	12.4177 mL	24.8355 mL
5 mM	0.4967 mL	2.4835 mL	4.9671 mL
10 mM	0.2484 mL	1.2418 mL	2.4835 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (250 KB) HNMR (274 KB) RP-HPLC (239 KB) MS (84 KB)

Handling Instructions (2659 KB)

References

[1]. Anne Vejux, et al. 7-Ketocholesterol and 7β-hydroxycholesterol: In vitro and animal models used to characterize their activities and to identify molecules preventing their toxicity. Biochem Pharmacol. 2020 Mar;173:113648. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4835 mL	12.4177 mL	24.8355 mL	62.0887 mL
	5 mM	0.4967 mL	2.4835 mL	4.9671 mL	12.4177 mL
	10 mM	0.2484 mL	1.2418 mL	2.4835 mL	6.2089 mL
	15 mM	0.1656 mL	0.8278 mL	1.6557 mL	4.1392 mL
	20 mM	0.1242 mL	0.6209 mL	1.2418 mL	3.1044 mL
	25 mM	0.0993 mL	0.4967 mL	0.9934 mL	2.4835 mL
	30 mM	0.0828 mL	0.4139 mL	0.8278 mL	2.0696 mL
	40 mM	0.0621 mL	0.3104 mL	0.6209 mL	1.5522 mL
	50 mM	0.0497 mL	0.2484 mL	0.4967 mL	1.2418 mL
	60 mM	0.0414 mL	0.2070 mL	0.4139 mL	1.0348 mL
	80 mM	0.0310 mL	0.1552 mL	0.3104 mL	0.7761 mL
	100 mM	0.0248 mL	0.1242 mL	0.2484 mL	0.6209 mL