Anisodamine (Synonyms: 6-Hydroxyhyoscyamine)

Name: Anisodamine
Brand: MedChemExpress
SKU: HY-N0584
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0584 Purity: 98.05%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Anisodamine (6-Hydroxyhyoscyamine), a belladonna alkaloid, is a non-subtype-selective muscarinic, and also a nicotinic cholinoceptor antagonist. Anisodamine employs in traditional Chinese medicine for many ailments, mainly to improve the microcirculation in states of shock, and also in organophosphate poisoning.

For research use only. We do not sell to patients.

Anisodamine Chemical Structure

CAS No. : 55869-99-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 418	Get quote	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 418	Get quote	Estimated Time of Arrival: December 31
Solid
1 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 380	Get quote	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Anisodamine:

Anisodamine hydrobromide Get quote
Anisodamine hydrochloride Get quote

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Nicotinic cholinoceptor^[1]

In Vitro

Anisodamine (100 μg/mL; 20 minutes; RAW264.7 cells) pretreatment for 20 minutes before Ach results in significantly attenuates average fluorescence intensity of α-bungarotoxin binding compared with Ach alone^[2].
Anisodamine action might be through blockade of muscarinic receptors and thus allowing more endogenous ACh binding to the α-7nAChR^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anisodamine (50 mg/kg; i.p.; 72 hours) markedly decreases the mortality to 20%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LPS-Induced Shock Mice^[2]
Dosage:	50 mg/kg
Administration:	I.p.
Result:	Markedly decreased the mortality to 20%.

Clinical Trial

Molecular Weight

305.37

Formula

C₁₇H₂₃NO₄

CAS No.

55869-99-3

Appearance

Solid

Color

White to off-white

SMILES

CN1[C@H]2[C@@H](O)C[C@@H]1C[C@H](OC([C@@H](C3=CC=CC=C3)CO)=O)C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (327.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (65.49 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2747 mL	16.3736 mL	32.7472 mL
5 mM	0.6549 mL	3.2747 mL	6.5494 mL
10 mM	0.3275 mL	1.6374 mL	3.2747 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (163.74 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.05%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (250 KB) HNMR (270 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Eisenkraft A, et al. Possible role for anisodamine in organophosphate poisoning. Br J Pharmacol. 2016 Jun;173(11):1719-27. [Content Brief]

[2]. Liu C, et al. Antishock effect of anisodamine involves a novel pathway for activating alpha7 nicotinic acetylcholine receptor. Crit Care Med. 2009;37(2):634-641. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.2747 mL	16.3736 mL	32.7472 mL	81.8679 mL
	5 mM	0.6549 mL	3.2747 mL	6.5494 mL	16.3736 mL
	10 mM	0.3275 mL	1.6374 mL	3.2747 mL	8.1868 mL
	15 mM	0.2183 mL	1.0916 mL	2.1831 mL	5.4579 mL
	20 mM	0.1637 mL	0.8187 mL	1.6374 mL	4.0934 mL
	25 mM	0.1310 mL	0.6549 mL	1.3099 mL	3.2747 mL
	30 mM	0.1092 mL	0.5458 mL	1.0916 mL	2.7289 mL
	40 mM	0.0819 mL	0.4093 mL	0.8187 mL	2.0467 mL
	50 mM	0.0655 mL	0.3275 mL	0.6549 mL	1.6374 mL
	60 mM	0.0546 mL	0.2729 mL	0.5458 mL	1.3645 mL
DMSO	80 mM	0.0409 mL	0.2047 mL	0.4093 mL	1.0233 mL
DMSO	100 mM	0.0327 mL	0.1637 mL	0.3275 mL	0.8187 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Anisodamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anisodamine55869-99-36-HydroxyhyoscyaminemAChRMuscarinic acetylcholine receptormuscarinicacetylcholinereceptorbelladonnaalkaloidChinesemedicinalherbScopoliatanguticaMaximInhibitorinhibitorinhibit

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Anisodamine (Synonyms: 6-Hydroxyhyoscyamine)

Customer Review

Other Forms of Anisodamine:

Anisodamine Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Anisodamine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Anisodamine Related Classifications

Keywords:

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