2. Cell Cycle/DNA Damage MAPK/ERK Pathway Anti-infection Apoptosis
  3. DNA/RNA Synthesis JNK Bacterial Apoptosis Antibiotic Parasite
  4. Anisomycin

Anisomycin  (Synonyms: Flagecidin; Wuningmeisu C)

Cat. No.: HY-18982 Purity: 99.82%
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Anisomycin is a potent protein synthesis inhibitor which interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system. Anisomycin is a JNK activator, which increases phospho-JNK. Anisomycin is a bacterial antibiotic.

For research use only. We do not sell to patients.

Anisomycin Chemical Structure

Anisomycin Chemical Structure

CAS No. : 22862-76-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Customer Review

Based on 84 publication(s) in Google Scholar

Anisomycin Related Antibodies

Top Publications Citing Use of Products

78 Publications Citing Use of MCE Anisomycin

WB

    Anisomycin purchased from MedChemExpress. Usage Cited in: Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C225-C235.  [Abstract]

    Western blot analysis shows that inhibiting MAPK cannot completely reverse increased expression of RNF2 and CDDP resistance of OC cells.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2018 Sep 5;834:295-304.  [Abstract]

    The protein levels of p-JNK by western blot following Anisomycin (4 μM) treatment for 24 h after Morroniside (100 μM) 12 h.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2018 Apr 23;8(1):6379.  [Abstract]

    Immunoblot analysis of lysates from Anisomycin pre-treatment cells reveals an increase of CHIP protein compared with OGD treated only cells, contrasting with a decrease in RIPK3 and p-MLKL protei. Pre-treatment cells with SP600125 results in the expected attenuation in JNK phosphorylation levels.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):467-473.  [Abstract]

    The increase of Bcl-2 and decrease of Bax, cleaved Caspase-3 and PARP induced by Ulk1- inhibition in LPS-exposed AST are abrogated by the Dehydrocorydaline chloride (DHC) or Anisomycin (ANS) pre-treatment

    Anisomycin purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 May 23;499(4):743-750.  [Abstract]

    Astrocytes are cultured with p38 and JNK activator of DHC and ANS, respectively, at the indicated concentrations for 24 h. Then, all cells are harvested for western blot analysis of p-p38 and p-JNK. Astrocytes are pretreated with DHC (500 nM) or ANS (10 mM) for 24 h, followed by Fru (5 mM) for an additional 24 h.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2017 Nov 1;277:62-73.  [Abstract]

    Effects of silencing p53 on colistin-induced ROS production and p-JNK expression level in PC-12 cells. The protein levels of p53. The protein levels of p-JNK by western blot.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Anisomycin is a potent protein synthesis inhibitor which interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system[1]. Anisomycin is a JNK activator, which increases phospho-JNK[2][3]. Anisomycin is a bacterial antibiotic[4].

    IC50 & Target[1][3]

    JNK

     

    DNA synthesis

     

    Cellular Effect
    Cell Line Type Value Description References
    Vero C1008 CC50
    < 0.39 μM
    Compound: Anisomycin
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 CC50
    < 0.39 μM
    Compound: Anisomycin
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    In Vitro

    To examine whether JNK has a core role in colistin-induced neurotoxicity in PC-12 cells, an SP600125 (a highly selective inhibitor of JNK) and Anisomycin (a potent activator) are used in this study. In order to select an appropriate concentration, PC-12 cells are treated with a range of SP600125 (0-80 μM) and Anisomycin (0-20 μM) respectively for 24 h. The results show that the cells viability significantly decreases by SP600125 treatment in a concentration-dependent manner, observed at the concentrations greater than 20 μM (p<0.01). Similarly the cells viability is inhibited by Anisomycin treatment (≥8 μM) (p<0.05) [1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Anisomycin Related Antibodies
    In Vivo

    Anisomycin (60 mg/kg; for 4-week continuous intravenous administration) significantly decreases mouse body weight in a dose-related manner, compared with the control group. Anisomycin (15 mg/kg; for 4-week continuous intravenous administration) slightly and transiently decreases the mouse body weight. There is no significant difference of the mouse body weight in 5 mg/kg group[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Balb/c mice of both sexes (4-5 weeks old)[3]
    Dosage: 84, 99, 116, 136 or 160 mg/kg; 0.2 mL per mouse
    Administration: Intravenously injected through mouse tail vein
    Result: The calculated LD50 for Anisomycin was 119.64 mg/kg.
    Molecular Weight

    265.31

    Formula

    C14H19NO4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@@H](OC(C)=O)[C@@H](CC2=CC=C(OC)C=C2)NC1
    Structure Classification
    Initial Source

    Streptomyces griseolus
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (188.46 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 4 mg/mL (15.08 mM; ultrasonic and warming and heat to 60°C)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7692 mL 18.8459 mL 37.6918 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 4 mg/mL (15.08 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.82%

    SDS (479 KB)

    COA (251 KB) HNMR (247 KB) RP-HPLC (243 KB) LCMS (86 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.7692 mL 18.8459 mL 37.6918 mL 94.2294 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL 18.8459 mL
    10 mM 0.3769 mL 1.8846 mL 3.7692 mL 9.4229 mL
    15 mM 0.2513 mL 1.2564 mL 2.5128 mL 6.2820 mL
    DMSO 20 mM 0.1885 mL 0.9423 mL 1.8846 mL 4.7115 mL
    25 mM 0.1508 mL 0.7538 mL 1.5077 mL 3.7692 mL
    30 mM 0.1256 mL 0.6282 mL 1.2564 mL 3.1410 mL
    40 mM 0.0942 mL 0.4711 mL 0.9423 mL 2.3557 mL
    50 mM 0.0754 mL 0.3769 mL 0.7538 mL 1.8846 mL
    60 mM 0.0628 mL 0.3141 mL 0.6282 mL 1.5705 mL
    80 mM 0.0471 mL 0.2356 mL 0.4711 mL 1.1779 mL
    100 mM 0.0377 mL 0.1885 mL 0.3769 mL 0.9423 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Anisomycin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Anisomycin22862-76-6Flagecidin Wuningmeisu CDNA/RNA SynthesisJNKBacterialApoptosisAntibioticParasitec-Jun N-terminal kinaseInhibitorinhibitorinhibit

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