2. Neuronal Signaling
  3. Beta-secretase
  4. BCA

BCA  (Synonyms: Disodium bicinchoninate)

Cat. No.: HY-15908 Purity: ≥98.0%
SDS COA Handling Instructions

Molarity Calculator

BCA (Disodium bicinchoninate) is an orally active and non-competitive Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) inhibitor with an IC50 value of 28 µM, a Ki value of 43 µM. BCA shows anticancer activity. BCA has the potential for the research of Alzheimer's disease (AD).

For research use only. We do not sell to patients.

BCA Chemical Structure

BCA Chemical Structure

CAS No. : 979-88-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 28 In-stock
Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
500 mg USD 25 In-stock
1 g USD 40 In-stock
5 g USD 130 In-stock
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

BCA Related Antibodies

Top Publications Citing Use of Products

View All Beta-secretase Isoform Specific Products:

View All Isoforms
BACE1 BACE2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BCA (Disodium bicinchoninate) is an orally active and non-competitive Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) inhibitor with an IC50 value of 28 µM, a Ki value of 43 µM. BCA shows anticancer activity. BCA has the potential for the research of Alzheimer's disease (AD)[1][2][3].

IC50 & Target[3]

BACE1

28 μM (IC50)

BACE1

43 μM (Ki)

In Vivo

BCA (5, 15 mg/kg; i.p.; daily) inhibits tumor growth in a xenograft animal model[1].
BCA (20, 40 mg/kg; p.o.; daily for 90 days) reduces the reduction of mitochondrial biogenesis, imbalances of mitochondrial dynamics, and abnormal mitophagy caused by ovariectomy[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week-old female athymic nude mice[1]
Dosage: 5, 15 mg/kg
Administration: I.p.; daily
Result: Reduced the incidence and the growth of estrogen-dependent MCF-7 tumors in a breast cancer xenograft mouse model.
Animal Model: APP/PS1 mice underwent bilateral ovariectomy[2]
Dosage: 20, 40 mg/kg
Administration: P.o.; daily for 90 days
Result: Rescued the reduction of mitochondrial biogenesis, imbalanced of mitochondrial dynamics, and abnormal mitophagy caused by ovariectomy.
Molecular Weight

388.28

Formula

C20H10N2Na2O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O[Na])C1=CC(C2=NC3=C(C=CC=C3)C(C(O[Na])=O)=C2)=NC4=C1C=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (257.55 mM; Need ultrasonic)

7.5% Sodium bicarbonate : ≥ 50 mg/mL (128.77 mM)

DMSO : 2 mg/mL (5.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5755 mL 12.8773 mL 25.7546 mL
5 mM 0.5151 mL 2.5755 mL 5.1509 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 25 mg/mL (64.39 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.76%

SDS (393 KB)

COA (252 KB) HNMR (131 KB) RP-HPLC (223 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / 7.5% Sodium bicarbonate / H2O 1 mM 2.5755 mL 12.8773 mL 25.7546 mL 64.3865 mL
5 mM 0.5151 mL 2.5755 mL 5.1509 mL 12.8773 mL
7.5% Sodium bicarbonate / H2O 10 mM 0.2575 mL 1.2877 mL 2.5755 mL 6.4387 mL
15 mM 0.1717 mL 0.8585 mL 1.7170 mL 4.2924 mL
20 mM 0.1288 mL 0.6439 mL 1.2877 mL 3.2193 mL
25 mM 0.1030 mL 0.5151 mL 1.0302 mL 2.5755 mL
30 mM 0.0858 mL 0.4292 mL 0.8585 mL 2.1462 mL
40 mM 0.0644 mL 0.3219 mL 0.6439 mL 1.6097 mL
50 mM 0.0515 mL 0.2575 mL 0.5151 mL 1.2877 mL
60 mM 0.0429 mL 0.2146 mL 0.4292 mL 1.0731 mL
80 mM 0.0322 mL 0.1610 mL 0.3219 mL 0.8048 mL
100 mM 0.0258 mL 0.1288 mL 0.2575 mL 0.6439 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

BCA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCA979-88-4Disodium bicinchoninateBeta-secretaseBACEβ-Secretaseorally activeanticancerAlzheimer's diseaseAPP/PS1 miceInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
BCA
Cat. No.:
HY-15908
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.