TZ9

Name: TZ9
Brand: MedChemExpress
SKU: HY-18643
Rating: 5.0 (2 reviews)

Cat. No.: HY-18643 Purity: 99.31%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TZ9 is a selective Rad6 inhibitor. TZ9 inhibits Rad6B-induced histone H2A ubiquitination, downregulates intracellular β-catenin, induces G2-M arrest and apoptosis, and inhibits the proliferation and migration of metastatic human breast cancer cells.

For research use only. We do not sell to patients.

TZ9 Chemical Structure

CAS No. : 1002789-86-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 560	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE TZ9

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	7.8 μM Compound: TZ9	Cytotoxicity against human A2780 cells after 72 hrs by MTS assay Cytotoxicity against human A2780 cells after 72 hrs by MTS assay	[PMID: 26965855]
A549	IC₅₀	7.2 μM Compound: TZ9	Cytotoxicity against human A549 cells after 72 hrs by MTS assay Cytotoxicity against human A549 cells after 72 hrs by MTS assay	[PMID: 26965855]
HT-29	IC₅₀	8.3 μM Compound: TZ9	Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 26965855]
MCF7	IC₅₀	5 μM Compound: TZ9	Cytotoxicity against human MCF7 cells after 72 hrs by MTS assay Cytotoxicity against human MCF7 cells after 72 hrs by MTS assay	[PMID: 26965855]
MDA-MB-231	GI₅₀	10 μM Compound: 20	Growth inhibition of human MDA-MB-231 cells after 72 hrs by MTT assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 27362876]
MDA-MB-231	IC₅₀	4.6 μM Compound: TZ9	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTS assay	[PMID: 26965855]
MDA-MB-231	IC₅₀	4.6 μM Compound: SMI#9	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35981459]
NCI-H1299	IC₅₀	45 μM Compound: TZ9	Cytotoxicity against human H1299 cells after 72 hrs by MTS assay Cytotoxicity against human H1299 cells after 72 hrs by MTS assay	[PMID: 26965855]
OV-90	IC₅₀	60 μM Compound: TZ9	Cytotoxicity against human OV90 cells after 72 hrs by MTS assay Cytotoxicity against human OV90 cells after 72 hrs by MTS assay	[PMID: 26965855]

In Vitro

TZ9 (Rad6B SMI #9) (0.5-100 µM; 72 h) inhibits MDA-MB-231 cell proliferation and migration^[1].
TZ9 (0.1-5 µM; 24-72 h) delays cell-cycle progression in MDA-MB-231 cells^[1].
TZ9 (5 µM; 8-48 h) induces apoptosis in MDA-MB-231 cells^[1].
TZ9 (0.5, 1, 2.5, 5 µM; 24 h) inhibits H2A ubiquitination and downregulates levels of PCNA and β-catenin protein in MDA-MB-231 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0.5-100 µM
Incubation Time:	72 h
Result:	Inhibited MDA-MB-231 cell proliferation with IC₅₀ ~6µM.

Apoptosis Analysis^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	5 µM
Incubation Time:	8-48 h
Result:	Induced cells apoptosis.

Cell Cycle Analysis^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0.1-5 µM
Incubation Time:	24-72 h
Result:	Increased the proportion of G2-M-arrested cells by 2-fold and was accompanied by a proportional decrease in S-phase cells when at 24 h. Significantly increased the percentage of cells with cytoplasmic/nuclear cyclin B1 staining.

Western Blot Analysis^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	0.5, 1, 2.5, 5 µM
Incubation Time:	24 h
Result:	Inhibited H2A ubiquitination, decreased PCNA and β-catenin protein levels.

Molecular Weight

366.33

Formula

C₁₇H₁₄N₆O₄

CAS No.

1002789-86-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(OCC1=NC(N)=NC(NC2=CC=CC=C2)=N1)C3=CC=C([N+]([O-])=O)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 40 mg/mL (109.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7298 mL	13.6489 mL	27.2978 mL
5 mM	0.5460 mL	2.7298 mL	5.4596 mL
10 mM	0.2730 mL	1.3649 mL	2.7298 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (245 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Sanders MA, et al. Novel inhibitors of Rad6 ubiquitin conjugating enzyme: design, synthesis, identification, and functional characterization. Mol Cancer Ther. 2013 Apr;12(4):373-83. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7298 mL	13.6489 mL	27.2978 mL	68.2445 mL
	5 mM	0.5460 mL	2.7298 mL	5.4596 mL	13.6489 mL
	10 mM	0.2730 mL	1.3649 mL	2.7298 mL	6.8244 mL
	15 mM	0.1820 mL	0.9099 mL	1.8199 mL	4.5496 mL
	20 mM	0.1365 mL	0.6824 mL	1.3649 mL	3.4122 mL
	25 mM	0.1092 mL	0.5460 mL	1.0919 mL	2.7298 mL
	30 mM	0.0910 mL	0.4550 mL	0.9099 mL	2.2748 mL
	40 mM	0.0682 mL	0.3412 mL	0.6824 mL	1.7061 mL
	50 mM	0.0546 mL	0.2730 mL	0.5460 mL	1.3649 mL
	60 mM	0.0455 mL	0.2275 mL	0.4550 mL	1.1374 mL
	80 mM	0.0341 mL	0.1706 mL	0.3412 mL	0.8531 mL
	100 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6824 mL

TZ9 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TZ9

Customer Review

TZ9 Related Antibodies

2 Publications Citing Use of MCE TZ9

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•Related Screening Libraries:

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References

Customer Review

TZ9 Related Antibodies

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Complete Stock Solution Preparation Table

TZ9 Related Classifications

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