BMX-IN-1 (Synonyms: BMX kinase inhibitor)

Name: BMX-IN-1
Brand: MedChemExpress
SKU: HY-80002
Rating: 5.0 (6 reviews)

Cat. No.: HY-80002 Purity: 98.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BMX-IN-1 is a selective, irreversible inhibitor of bone marrow tyrosine kinase on chromosome X (BMX) that targets Cys⁴⁹⁶ in the BMX ATP binding domain with an IC₅₀ of 8 nM, also targets the related Bruton’s tyrosine kinase (BTK) with an IC₅₀ value of 10.4 nM, but is more than 47-656-fold less potent against Blk, JAK3, EGFR, Itk, or Tec activity.

For research use only. We do not sell to patients.

BMX-IN-1 Chemical Structure

CAS No. : 1431525-23-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 196	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 196	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 157	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 252	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

5 Publications Citing Use of MCE BMX-IN-1

: BMX-IN-1 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jul 25;8(30):49238-49252. [Abstract]; Western blot results show that the expression of BMX is decreased in both BMX-IN-1-treated HeLa and SiHa cells.

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Description

IC₅₀ & Target

IC50: 8 nM (BMX), 10.4 nM (BTK)

In Vitro

BMX-IN-1 inhibits the proliferation of Tel-BMX-transformed Ba/F3 cells and RV-1 cells with IC₅₀s of 25 nM and 2.53 μM. BMX-IN-1 exhibits remarkable selectivity with an S(10) score of 0.01. BMX-IN-1 inhibits only wild-type BMX with an IC₅₀ of 138 nM. BMX-IN-1 requires covalent modification of Cys⁴⁹⁶ of BMX to achieve potent inhibition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

524.59

Formula

C₂₉H₂₄N₄O₄S

CAS No.

1431525-23-3

Appearance

Solid

Color

White to yellow

SMILES

O=C1N(C2=C(C=C1)C=NC3=CC=C(C4=CC=C(NS(=O)(C)=O)C=C4)C=C32)C5=CC=C(C)C(NC(C=C)=O)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMF : 10 mg/mL (19.06 mM; Need ultrasonic)

DMSO : 8.33 mg/mL (15.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9063 mL	9.5313 mL	19.0625 mL
5 mM	0.3813 mL	1.9063 mL	3.8125 mL
10 mM	0.1906 mL	0.9531 mL	1.9063 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (19.06 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.04%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (257 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Feiyang Liu , et al. Discovery of a Selective Irreversible BMX Inhibitor for Prostate Cancer. ACS Chem. Biol., DOI: 10.1021/cb4000629

Cell Assay
^[1]

RV-1 cells in complete or serum-reduced DMEM are treated with DMSO, BMX-IN-1 (2.5 μM), MK2206 (200 nM), or the combination of BMX-IN-1 and MK2206 for 5 days before cells are harvested by trypsin and washed with cold PBS. The cells are then fixed in 70% cold ethanol (prechilled at −20°C) and incubated at 4°C overnight. On the day of flow cytometry, cells are collected by centrifugation, washed with PBS, and stained in 50 μg/mL propidium iodide + 0.5 mg/mL RNase in PBS + 0.5% Triton-X100 for 30 min at RT and moved to 4°C until the time of analysis. Flow cytometry is performed using a BD FACScan, and results are analyzed by ModFit software in the Flow Cytometry Core Facility in Dana-Faber Cancer Institute.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Feiyang Liu , et al. Discovery of a Selective Irreversible BMX Inhibitor for Prostate Cancer. ACS Chem. Biol., DOI: 10.1021/cb4000629

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / DMF	1 mM	1.9063 mL	9.5313 mL	19.0625 mL	47.6563 mL
	5 mM	0.3813 mL	1.9063 mL	3.8125 mL	9.5313 mL
	10 mM	0.1906 mL	0.9531 mL	1.9063 mL	4.7656 mL
	15 mM	0.1271 mL	0.6354 mL	1.2708 mL	3.1771 mL

BMX-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BMX-IN-11431525-23-3BMX kinase inhibitorBtkBMX KinaseBruton tyrosine kinaseInhibitorinhibitorinhibit

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BMX-IN-1 (Synonyms: BMX kinase inhibitor)

Customer Review

BMX-IN-1 Related Antibodies

5 Publications Citing Use of MCE BMX-IN-1

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

BMX-IN-1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

BMX-IN-1 Related Classifications

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