Bobcat339 hydrochloride

Name: Bobcat339 hydrochloride
Brand: MedChemExpress
SKU: HY-111558A
Rating: 5.0 (19 reviews)

Cat. No.: HY-111558A Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bobcat339 hydrochloride is a potent and selective cytosine-based inhibitor of TET enzyme, with the IC₅₀s of 33 μM and 73 μM for TET1 and TET2, respectively. Bobcat339 hydrochloride is useful to the field of epigenetics and serves as a starting point for new therapeutics that target DNA methylation and gene transcription.

For research use only. We do not sell to patients.

Bobcat339 hydrochloride Chemical Structure

CAS No. : 2436747-44-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 19 publication(s) in Google Scholar

Other Forms of Bobcat339 hydrochloride:

Bobcat339 Get quote

18 Publications Citing Use of MCE Bobcat339 hydrochloride

RT-PCR

: Bobcat339 hydrochloride purchased from MedChemExpress. Usage Cited in: Aquaculture. 2023: 739234.; Bobcat 339 (4 mM; every 48 h for a total of 7 injections) inject into the molluscum of the oyster utilizing a syringe, the expression levels of Sox-like and Sox8 are significantly increased, which are 1.93 and 1.47 times higher than the control group, respectively.

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TET1 TET2 TET3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

TET1

33 μM (IC₅₀)

TET2

73 μM (IC₅₀)

In Vitro

Bobcat339 (10 μM; 24 hours) significantly reduces global 5hmC levels by inhibiting TET enzyme function in HT-22 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

334.20

Formula

C₁₆H₁₃Cl₂N₃O

CAS No.

2436747-44-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

NC1=NC(N(C2=CC=CC(C3=CC=CC=C3)=C2)C=C1Cl)=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (374.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9922 mL	14.9611 mL	29.9222 mL
5 mM	0.5984 mL	2.9922 mL	5.9844 mL
10 mM	0.2992 mL	1.4961 mL	2.9922 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (248 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Chua GNL, et al. Cytosine-Based TET Enzyme Inhibitors. ACS Med Chem Lett. 2019 Jan 31;10(2):180-185. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9922 mL	14.9611 mL	29.9222 mL	74.8055 mL
	5 mM	0.5984 mL	2.9922 mL	5.9844 mL	14.9611 mL
	10 mM	0.2992 mL	1.4961 mL	2.9922 mL	7.4806 mL
	15 mM	0.1995 mL	0.9974 mL	1.9948 mL	4.9870 mL
	20 mM	0.1496 mL	0.7481 mL	1.4961 mL	3.7403 mL
	25 mM	0.1197 mL	0.5984 mL	1.1969 mL	2.9922 mL
	30 mM	0.0997 mL	0.4987 mL	0.9974 mL	2.4935 mL
	40 mM	0.0748 mL	0.3740 mL	0.7481 mL	1.8701 mL
	50 mM	0.0598 mL	0.2992 mL	0.5984 mL	1.4961 mL
	60 mM	0.0499 mL	0.2494 mL	0.4987 mL	1.2468 mL
	80 mM	0.0374 mL	0.1870 mL	0.3740 mL	0.9351 mL
	100 mM	0.0299 mL	0.1496 mL	0.2992 mL	0.7481 mL