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Coixol  (Synonyms: 6-Methoxy-2-benzoxazolinone; 6-MBOA)

Cat. No.: HY-N0936 Purity: 99.58%
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Coixol (6-Methoxy-2-benzoxazolinone; 6-MBOA) is a potent and orally active anti-inflammatory agent. Coixol decreases the iNOS protein expression. Coixol inhibits the production of TNF-α, IL-6, and IL-1β. Coixol improves glucose tolerance and plasma insulin. Coixol decreases the blood glucose level.

For research use only. We do not sell to patients.

Coixol Chemical Structure

Coixol Chemical Structure

CAS No. : 532-91-2

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Coixol Related Antibodies

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Description

Coixol (6-Methoxy-2-benzoxazolinone; 6-MBOA) is a potent and orally active anti-inflammatory agent. Coixol decreases the iNOS protein expression. Coixol inhibits the production of TNF-α, IL-6, and IL-1β. Coixol improves glucose tolerance and plasma insulin. Coixol decreases the blood glucose level[1][2][3].

In Vitro

Coixol (5, 10, 20 µM, 2+24 h) decreases the iNOS protein expression in LPS-induced RAW264.7 Cells[2].
Coixol (0.03, 0.1, 0.3, 1, 3, 10, 30, 100 μM; 1+24 h) inhibits the production of TNF-α, IL-6, and IL-1β with IC50s of 31.2, 48.9, 66.4 µM, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coixol Related Antibodies

Western Blot Analysis[2]

Cell Line: RAW264.7 cells
Concentration: 5, 10, 20 µM
Incubation Time: 2 h and then stimulated with or without LPS (0.5 μg/mL) for 24 h
Result: Decreased the iNOS protein expression in a dose dependent manner.
In Vivo

Coixol (25, 50 mg/kg, p.o.) improves glucose tolerance and stimulates glucose-induced plasma insulin in non-diabetic and diabetic rats[3].
Coixol (25, 50 mg/kg, p.o.; daily for 15 day) decreases the blood glucose levels in a dose- and time-dependent manner in type 2 diabetic rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 9–12 weeks, Sprague Dawley (SD) male rats[3]
Dosage: 25, 50 mg/kg
Administration: P.o.
Result: Decreased the blood glucose levels significantly, improved glucose tolerance.
Molecular Weight

165.15

Formula

C8H7NO3
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=C1OC2=CC(OC)=CC=C2N1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (605.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.0551 mL 30.2755 mL 60.5510 mL
5 mM 1.2110 mL 6.0551 mL 12.1102 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (15.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (15.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.0551 mL 30.2755 mL 60.5510 mL 151.3775 mL
5 mM 1.2110 mL 6.0551 mL 12.1102 mL 30.2755 mL
10 mM 0.6055 mL 3.0276 mL 6.0551 mL 15.1378 mL
15 mM 0.4037 mL 2.0184 mL 4.0367 mL 10.0918 mL
20 mM 0.3028 mL 1.5138 mL 3.0276 mL 7.5689 mL
25 mM 0.2422 mL 1.2110 mL 2.4220 mL 6.0551 mL
30 mM 0.2018 mL 1.0092 mL 2.0184 mL 5.0459 mL
40 mM 0.1514 mL 0.7569 mL 1.5138 mL 3.7844 mL
50 mM 0.1211 mL 0.6055 mL 1.2110 mL 3.0276 mL
60 mM 0.1009 mL 0.5046 mL 1.0092 mL 2.5230 mL
80 mM 0.0757 mL 0.3784 mL 0.7569 mL 1.8922 mL
100 mM 0.0606 mL 0.3028 mL 0.6055 mL 1.5138 mL
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Coixol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coixol532-91-26-Methoxy-2-benzoxazolinone 6-MBOAOthersorally activeiNOSglucose toleranceinsulinblood glucoseInhibitorinhibitorinhibit

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