Dobutamine hydrochloride

Name: Dobutamine hydrochloride
Brand: MedChemExpress
SKU: HY-15746
Rating: 5.0 (3 reviews)

Cat. No.: HY-15746 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Dobutamine hydrochloride is a synthetic catecholamine that acts on α1-AR, β1-AR, β2-AR (α-1, β-1 andβ-2 adrenoceptors). Dobutamine hydrochloride is a selective β1-AR agonist, relatively weak activity at α1-AR and β2-AR. Dobutamine hydrochloride can increase cardiac output and correct hypoperfusion.

For research use only. We do not sell to patients.

Dobutamine hydrochloride Chemical Structure

CAS No. : 49745-95-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 174	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 174	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 158	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Dobutamine hydrochloride:

(rac)-Dobutamine-d₄ hydrochloride Get quote
(rac)-Dobutamine-d₆ hydrochloride Get quote
Dobutamine Get quote
Dobutamine tartrate Get quote
Dobutamine hydrochloride (Standard) Get quote

3 Publications Citing Use of MCE Dobutamine hydrochloride

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•Related Screening Libraries:

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View All Adrenergic Receptor Isoform Specific Products:

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α adrenergic receptor β adrenergic receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

α adrenergic receptor

β adrenergic receptor

In Vivo

Dobutamine hydrochloride has a rapid onset of action and a short half-life ^[2].
Dobutamine hydrochloride (0.15-20 mg/kg; i.p.) results in subsequent increase in the left ventricular function and heart rate acceleration with an increasing dose in wildtype mice^[3].
Dobutamine hydrochloride results in significant inotropic, lusitropic, and chronotropic cardiac response with a high dose in wildtype mice^[3].
Low doses of Dobutamine hydrochloride significantly increases inotropic and lusitropic cardiac performance without chronotropic changes in the Tgαq*44 mice^[3].
Dobutamine hydrochloride increases heart rate only after high doses, but then inotropic and lusitropic cardiac functional reserve is lost^[3].
Dobutamine hydrochloride increases alveolar liquid clearance in ventilated rats by beta-2 receptor stimulation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Tgαq*44 mice (heart failure models)^[3]
Dosage:	0.15 mg/kg, 0.5 mg/kg as a low dose, 1.5 mg/kg, 5 mg/kg, 20 mg/kg as a high dose
Administration:	Intraperitoneal injection
Result:	Induced different response in cardiac function on a low and high dose in mice with with heart failure.

Clinical Trial

Molecular Weight

337.84

Formula

C₁₈H₂₄ClNO₃

CAS No.

49745-95-1

Appearance

Solid

Color

White to gray

SMILES

OC1=CC=C(CCNC(C)CCC2=CC=C(O)C=C2)C=C1O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 33 mg/mL (97.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (59.20 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9600 mL	14.7999 mL	29.5998 mL
5 mM	0.5920 mL	2.9600 mL	5.9200 mL
10 mM	0.2960 mL	1.4800 mL	2.9600 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (49.34 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (418 KB)

COA (244 KB) HNMR (262 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Tuttle RR, et al. Dobutamine: development of a new catecholamine to selectively increase cardiac contractility. Circ Res. 1975 Jan;36(1):185-96. [Content Brief]

[2]. Vallet B, et al. Dobutamine: mechanisms of action and use in acute cardiovascular pathology. Ann Cardiol Angeiol (Paris). 1991 Jun;40(6):397-402. [Content Brief]

[3]. Tyrankiewicz U , et al. Characterization of the cardiac response to a low and high dose of dobutamine in the mouse model of dilated cardiomyopathy by MRI in vivo. J Magn Reson Imaging. 2013 Mar;37(3):669-77. [Content Brief]

[4]. Tibayan FA, et al. Dobutamine increases alveolar liquid clearance in ventilated rats by beta-2 receptor stimulation. Am J Respir Crit Care Med. 1997 Aug;156(2 Pt 1):438-44. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9600 mL	14.7999 mL	29.5998 mL	73.9995 mL
	5 mM	0.5920 mL	2.9600 mL	5.9200 mL	14.7999 mL
	10 mM	0.2960 mL	1.4800 mL	2.9600 mL	7.4000 mL
	15 mM	0.1973 mL	0.9867 mL	1.9733 mL	4.9333 mL
	20 mM	0.1480 mL	0.7400 mL	1.4800 mL	3.7000 mL
	25 mM	0.1184 mL	0.5920 mL	1.1840 mL	2.9600 mL
	30 mM	0.0987 mL	0.4933 mL	0.9867 mL	2.4667 mL
	40 mM	0.0740 mL	0.3700 mL	0.7400 mL	1.8500 mL
	50 mM	0.0592 mL	0.2960 mL	0.5920 mL	1.4800 mL
DMSO	60 mM	0.0493 mL	0.2467 mL	0.4933 mL	1.2333 mL
DMSO	80 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9250 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.