Ebselen (Synonyms: SPI-1005; PZ-51; CCG-39161)

Name: Ebselen
Brand: MedChemExpress
SKU: HY-13750
Rating: 5.0 (14 reviews)

Cat. No.: HY-13750 Purity: 99.58%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ebselen (SPI-1005), a glutathione peroxidase mimetic, is a potent voltage-dependent calcium channel (VDCC) blocker. Ebselen potently inhibits M^pro (IC₅₀=0.67 μM) and COVID-19 virus (EC₅₀=4.67 μM).Ebselen is an inhibitor of HIV-1 capsid CTD dimerization. Ebselen, an organoselenium compound, can permeate the blood-brain barrier and has anti-inflammatory, antioxidant and anticancer activity.

For research use only. We do not sell to patients.

Ebselen Chemical Structure

CAS No. : 60940-34-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 14 publication(s) in Google Scholar

13 Publications Citing Use of MCE Ebselen

: Ebselen purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2019 Feb;17(2):1412-1419. [Abstract]; Ebselen influences the expression of apoptosis‑related proteins. The effects of ebselen on C‑Caspase‑8, C‑Caspase‑3, Bax, Bcl‑2 and C‑PARP are analyzed by western blotting.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Calcium Channel Isoform Specific Products:

View All Isoforms

N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

View All HIV Isoform Specific Products:

View All Isoforms

HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Ebselen (SPI-1005), a glutathione peroxidase mimetic, is a potent voltage-dependent calcium channel (VDCC) blocker^[1]^[2]. Ebselen potently inhibits M^pro (IC₅₀=0.67 μM) and COVID-19 virus (EC₅₀=4.67 μM)^[3].Ebselen is an inhibitor of HIV-1 capsid CTD dimerization. Ebselen, an organoselenium compound, can permeate the blood-brain barrier and has anti-inflammatory, antioxidant and anticancer activity^[4]^[5].

IC₅₀ & Target

HIV-1

Cellular Effect

Cell Line	Type	Value	Description	References
184B5	GI₅₀	30.1 μM Compound: EBS	Antiproliferative activity against human 184B5 cells after 72 hrs by MTT assay Antiproliferative activity against human 184B5 cells after 72 hrs by MTT assay	[PMID: 30071406]
A549	IC₅₀	> 100 μM Compound: PZ51	Antiproliferative activity against human A549 cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 25817772]
A549	IC₅₀	16.8 μM Compound: Ebs	Cytotoxicity in human A549 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay Cytotoxicity in human A549 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay	[PMID: 29584939]
A549	IC₅₀	3.75 μM Compound: Ebselen	Antiproliferative activity against human A549 cells after 48 hrs by CCK-8 assay Antiproliferative activity against human A549 cells after 48 hrs by CCK-8 assay	[PMID: 22483583]
BEAS-2B	GI₅₀	49.5 μM Compound: EBS	Antiproliferative activity against human BEAS2B cells after 72 hrs by MTT assay Antiproliferative activity against human BEAS2B cells after 72 hrs by MTT assay	[PMID: 30071406]
Bel-7402	IC₅₀	68.2 μM Compound: PZ51	Antiproliferative activity against human Bel7402 cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human Bel7402 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 25817772]
Calu-1	GI₅₀	68.6 μM Compound: EBS	Antiproliferative activity against human HTB-54 cells after 72 hrs by MTT assay Antiproliferative activity against human HTB-54 cells after 72 hrs by MTT assay	[PMID: 30071406]
CCRF-CEM	GI₅₀	57.1 μM Compound: EBS	Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay	[PMID: 30071406]
CHO-K1	EC₅₀	6.1 μM Compound: 7	Cytotoxicity against CHO-K1 cells by Alamar blue assay Cytotoxicity against CHO-K1 cells by Alamar blue assay	[PMID: 16962325]
HCT-116	IC₅₀	50 μM Compound: Ebs	Cytotoxicity in human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay Cytotoxicity in human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay	[PMID: 29584939]
HeLa	IC₅₀	78.5 μM Compound: PZ51	Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 25817772]
HT-29	GI₅₀	> 100 μM Compound: EBS	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 30071406]
HT-29	IC₅₀	50.4 μM Compound: Ebs	Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay	[PMID: 29584939]
MCF7	IC₅₀	> 100 μM Compound: PZ51	Antiproliferative activity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 25817772]
MCF7	IC₅₀	15.02 μM Compound: Ebselen	Antiproliferative activity against human MCF7 cells after 48 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells after 48 hrs by CCK-8 assay	[PMID: 22483583]
MCF7	GI₅₀	58.8 μM Compound: EBS	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 30071406]
MDA-MB-231	IC₅₀	42.3 μM Compound: 1, PZ 51, ebselen	Antiproliferative activity against human MDA231 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA231 cells after 72 hrs by MTT assay	[PMID: 22204902]
NCI-H1299	IC₅₀	27.7 μM Compound: Ebs	Cytotoxicity in human NCI-H1299 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay Cytotoxicity in human NCI-H1299 cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based assay	[PMID: 29584939]
PC-3	GI₅₀	> 100 μM Compound: EBS	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 30071406]
U-266	IC₅₀	40 μM Compound: 62	Anticancer activity against human U-266 cells assessed as cell growth inhibition incubated for 24 hrs by MTS assay Anticancer activity against human U-266 cells assessed as cell growth inhibition incubated for 24 hrs by MTS assay	[PMID: 34217061]

In Vitro

Ebselen (SPI-1005; 0.4-100 μM; 20-24 hours) shows strong antiviral effects at a concentration of 10 μM treatment in COVID-19 virus infected Vero cells. Ebsele covalently binds to C145 of the catalytic dyad in COVID-19 virus Mpro^[3].
Ebselen inhibits early viral postentry events of the HIV-1 life cycle by impairing the incoming capsid uncoating process^[4].
Ebselen permeates the blood-brain barrier and inhibits endogenous inositol monophosphatase in mouse brain. Ebselen inhibits inositol monophosphatase (IMPase)^[5].
Ebselen inhibits QSOX1 enzymatic activity and suppresses invasion of pancreatic, renal cancer cell lines^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[3]

Cell Line:	COVID-19 virus infected Vero cells
Concentration:	0.4, 1.2, 3.7, 11.1, 33.3, 100 μM
Incubation Time:	20-24 hours
Result:	Showed strong antiviral effects at a concentration of 10 μM treatment.

In Vivo

Ebselen (5, 10 mg/kg; IP) decreases 5-HT₂ agonist-induced head twitches in a dose-dependent manner^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	20-25 g 10-12 week old male C57Bl6 mice^[5]
Dosage:	5, 10 mg/kg
Administration:	IP
Result:	Decreased 5-HT₂ agonist-induced head twitches in a dose-dependent manner.

Clinical Trial

Molecular Weight

274.18

Formula

C₁₃H₉NOSe

CAS No.

60940-34-3

Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1N(C2=CC=CC=C2)[Se]C3=C1C=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (182.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6472 mL	18.2361 mL	36.4723 mL
5 mM	0.7294 mL	3.6472 mL	7.2945 mL
10 mM	0.3647 mL	1.8236 mL	3.6472 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (280 KB) SDS (788 KB)

COA (251 KB) HNMR (274 KB) LCMS (122 KB) Elemental Analysis Report (110 KB)

Handling Instructions (2659 KB)

References

[1]. Liang Q, et al. Electrical Stimulation Degenerated Cochlear Synapses Through Oxidative Stress in NeonatalCochlear Explants. Front Neurosci. 2019 Oct 14;13:1073. [Content Brief]

[2]. H Sies, et al. Ebselen, a Selenoorganic Compound as Glutathione Peroxidase Mimic [Content Brief]

[3]. Jin Z, et al. Structure of M^pro from COVID-19 virus and discovery of its inhibitors. Nature. 2020 Apr 9. [Content Brief]

[4]. Thenin-Houssier S, et al. Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication. Antimicrob Agents Chemother. 2016 Mar 25;60(4):2195-208. [Content Brief]

[5]. Singh N, et al. A safe lithium mimetic for bipolar disorder. Nat Commun. 2013;4:1332. doi: 10.1038/ncomms2320. [Content Brief]

[6]. Hanavan PD, et al. Ebselen inhibits QSOX1 enzymatic activity and suppresses invasion of pancreatic and renal cancer cell lines. Oncotarget. 2015 Jul 30;6(21):18418-28. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6472 mL	18.2361 mL	36.4723 mL	91.1806 mL
	5 mM	0.7294 mL	3.6472 mL	7.2945 mL	18.2361 mL
	10 mM	0.3647 mL	1.8236 mL	3.6472 mL	9.1181 mL
	15 mM	0.2431 mL	1.2157 mL	2.4315 mL	6.0787 mL
	20 mM	0.1824 mL	0.9118 mL	1.8236 mL	4.5590 mL
	25 mM	0.1459 mL	0.7294 mL	1.4589 mL	3.6472 mL
	30 mM	0.1216 mL	0.6079 mL	1.2157 mL	3.0394 mL
	40 mM	0.0912 mL	0.4559 mL	0.9118 mL	2.2795 mL
	50 mM	0.0729 mL	0.3647 mL	0.7294 mL	1.8236 mL
	60 mM	0.0608 mL	0.3039 mL	0.6079 mL	1.5197 mL
	80 mM	0.0456 mL	0.2280 mL	0.4559 mL	1.1398 mL
	100 mM	0.0365 mL	0.1824 mL	0.3647 mL	0.9118 mL

Ebselen Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社
Product Name			Salutation
Applicant Name *			Email Address *
Phone Number *			Organization Name *
Department *			Requested quantity *
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Ebselen (Synonyms: SPI-1005; PZ-51; CCG-39161)

Customer Review

13 Publications Citing Use of MCE Ebselen

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Calcium Channel Isoform Specific Products:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ebselen Related Classifications

Keywords:

Your Recently Viewed Products:

Name
Email *

	Sorry, but the email address you supplied was invalid.

Ebselen (Synonyms: SPI-1005; PZ-51; CCG-39161)

Customer Review

Ebselen Related Antibodies

13 Publications Citing Use of MCE Ebselen

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Calcium Channel Isoform Specific Products:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Ebselen Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ebselen Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report