2. Vitamin D Related/Nuclear Receptor Apoptosis
  3. Estrogen Receptor/ERR Apoptosis
  4. Estradiol cypionate

Estradiol cypionate is the 17β-cypionate ester of Estradiol, which inhibits ET-1 synthesis by acting on estrogen receptors.

For research use only. We do not sell to patients.

Estradiol cypionate Chemical Structure

Estradiol cypionate Chemical Structure

CAS No. : 313-06-4

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Estradiol cypionate:

Estradiol cypionate Related Antibodies

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  • Biological Activity

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  • References

  • Customer Review

Description

Estradiol cypionate is the 17β-cypionate ester of Estradiol, which inhibits ET-1 synthesis by acting on estrogen receptors[1].

In Vitro

Estradiol cypionate (0-30 μM, 72 h) inhibits proliferation of gastric cancer cells (MGC803, SGC7901, and BGC823)[4].
Estradiol cypionate (10-25 μM, 48 h) induces G1/S phase cell cycle arrest, and induces apoptosis by PI3K /Akt/mTOR pathway in gastric cancer cells (MGC803, SGC7901, and BGC823 cells)[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Estradiol cypionate Related Antibodies

Western Blot Analysis[4]

Cell Line: MGC803, SGC7901, and BGC823 cells
Concentration: 10-25 μM
Incubation Time: 48 h
Result: Increased protein level of cleaved caspase-3 and PARP.
Decreased protein levels of AKT, p-AKT, p-mTOR, p-S6K, and p-4E-BP1.
In Vivo

Estradiol cypionate (70 μg/kg, i.m., weekly) increases cortical bone density in pubertal ovariectomized (OVX) rabbits[2].
Estradiol cypionate (1 mg, i.m.) increases occurrence of estrus and pregnancy in cows[3].
Estradiol cypionate (50-100 mg/kg, i.p., every other day) inhibits tumor growth in MGC803 nude mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MGC803 nude mice tumor model[4].
Dosage: 50-100 mg/kg
Administration: i.p., every other day
Result: Inhibited tumor growth.
Increased Cleaved caspase-3 expression, decreased Ki67, AKT and p-AKT in tumors.
Molecular Weight

396.56

Formula

C26H36O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@@H](OC(CCC3CCCC3)=O)CC[C@@]1([H])[C@]4([H])CCC5=C(C=CC(O)=C5)[C@@]4([H])CC2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 30 mg/mL (75.65 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5217 mL 12.6084 mL 25.2169 mL
5 mM 0.5043 mL 2.5217 mL 5.0434 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.67 mg/mL (6.73 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (26.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.67 mg/mL (6.73 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (26.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.62%

SDS (419 KB)

COA (248 KB) HNMR (286 KB) LCMS (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5217 mL 12.6084 mL 25.2169 mL 63.0422 mL
5 mM 0.5043 mL 2.5217 mL 5.0434 mL 12.6084 mL
10 mM 0.2522 mL 1.2608 mL 2.5217 mL 6.3042 mL
15 mM 0.1681 mL 0.8406 mL 1.6811 mL 4.2028 mL
20 mM 0.1261 mL 0.6304 mL 1.2608 mL 3.1521 mL
25 mM 0.1009 mL 0.5043 mL 1.0087 mL 2.5217 mL
30 mM 0.0841 mL 0.4203 mL 0.8406 mL 2.1014 mL
40 mM 0.0630 mL 0.3152 mL 0.6304 mL 1.5761 mL
50 mM 0.0504 mL 0.2522 mL 0.5043 mL 1.2608 mL
60 mM 0.0420 mL 0.2101 mL 0.4203 mL 1.0507 mL
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Estradiol cypionate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Estradiol cypionate313-06-4Estrogen Receptor/ERRApoptosisMGC803SGC7901BGC823cancerInhibitorinhibitorinhibit

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