Hesperidin (Synonyms: Hesperetin 7-rutinoside)

Name: Hesperidin
Brand: MedChemExpress
SKU: HY-15337
Rating: 5.0 (13 reviews)

Cat. No.: HY-15337 Purity: 98.06%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities.

For research use only. We do not sell to patients.

Hesperidin Chemical Structure

CAS No. : 520-26-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 42	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 119	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 158	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 211	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 251	In-stock	Estimated Time of Arrival: December 31
1 g	USD 383	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Based on 13 publication(s) in Google Scholar

Other Forms of Hesperidin:

Hesperidin (Standard) Get quote

12 Publications Citing Use of MCE Hesperidin

: Hesperidin purchased from MedChemExpress. Usage Cited in: Cell Rep. 2017 Nov 21;21(8):2147-2159. [Abstract]; U2OS cells expressing Myc-tagged PLK1 WT, K492R, or SUMO-1-K492R are treated with NSC 125973 for 16 hr. Half of the cells are then released in the presence of Hesperidin for 45 min to enrich cells at the later stage of M phase. Cell lysates are subject to IP, followed by western blot.

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
DLD-1	IC₅₀	> 100 μM Compound: 12	Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
H9	EC₅₀	> 164 μM Compound: 21	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	> 164 μM Compound: 21	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
HT-29	IC₅₀	142 μM Compound: 11	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
Monocyte	IC₅₀	> 200 μM Compound: Hesperidin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

Hesperidin (5-200 μM; 24-72 h) induces potent cytotoxic effects in human osteosarcoma MG-63 cells^[1].
Hesperidin (5-150 μM; 48 h) induces early and late apoptosis in MG-63 cells^[1].
Hesperidin (10-30 μM) inhibits the activity of COX-2 and iNOS in a dose dependent manner in RAW 264.7 cells activated with LPS^[2].
Hesperidin (0.1 μg/mL; 2 h) decreases the formation of MDA and intracellular ROS, including chondrocyte apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MG-63 cells
Concentration:	0, 5, 25, 50, 100, 150, 200 µM
Incubation Time:	24, 48, 72 hours
Result:	Led to time-dependent and concentration-dependent cytotoxic effects, with IC₅₀s of 94.3, 78.6 and 63.3 µM at 24, 48 and 72 h, respectively.

Apoptosis Analysis^[1]

Cell Line:	MG-63 cells
Concentration:	0, 5, 50, 150 µM
Incubation Time:	48 hours
Result:	Increased the percentage of apoptotic cells from 4.7% to 17.9, 34.6 and 68.3% at the concentration of 0, 5, 50 and 150 µM, respectively.

In Vivo

Hesperidin (5-80 mg/kg; 2 weeks) significantly suppresses MG-63 tumor growth in mice^[1].
Hesperidin (200 mg/kg; once daily for 28 d) markedly attenuates cartilage destruction and reduces IL-1β and TNF-α levels in a surgically-induced osteoarthritis (OA) rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

610.56

Formula

C₂₈H₃₄O₁₅

CAS No.

520-26-3

Appearance

Solid

Color

Light yellow to khaki

SMILES

OC1=C(OC)C=CC([C@@H]2CC(C3=C(C=C(O[C@@H]4O[C@H](CO[C@@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)C=C3O)O2)=O)=C1

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (163.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6378 mL	8.1892 mL	16.3784 mL
5 mM	0.3276 mL	1.6378 mL	3.2757 mL
10 mM	0.1638 mL	0.8189 mL	1.6378 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (281 KB) SDS (394 KB)

COA (253 KB) HNMR (285 KB) RP-HPLC (257 KB) MS (86 KB)

Handling Instructions (2659 KB)

References

[1]. Du GY, et, al. Hesperidin exhibits in vitro and in vivo antitumor effects in human osteosarcoma MG-63 cells and xenograft mice models via inhibition of cell migration and invasion, cell cycle arrest and induction of mitochondrial-mediated apoptosis. Oncol Lett. 2018 Nov;16(5):6299-6306. [Content Brief]

[2]. Tejada S, et, al. Potential Anti-inflammatory Effects of Hesperidin from the Genus Citrus. Curr Med Chem. 2018;25(37):4929-4945. [Content Brief]

[3]. Gao G, et, al. Effects of Hesperidin on H₂O₂-Treated Chondrocytes and Cartilage in a Rat Osteoarthritis Model. Med Sci Monit. 2018 Dec 17;24:9177-9186. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6378 mL	8.1892 mL	16.3784 mL	40.9460 mL
	5 mM	0.3276 mL	1.6378 mL	3.2757 mL	8.1892 mL
	10 mM	0.1638 mL	0.8189 mL	1.6378 mL	4.0946 mL
	15 mM	0.1092 mL	0.5459 mL	1.0919 mL	2.7297 mL
	20 mM	0.0819 mL	0.4095 mL	0.8189 mL	2.0473 mL
	25 mM	0.0655 mL	0.3276 mL	0.6551 mL	1.6378 mL
	30 mM	0.0546 mL	0.2730 mL	0.5459 mL	1.3649 mL
	40 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	50 mM	0.0328 mL	0.1638 mL	0.3276 mL	0.8189 mL
	60 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6824 mL
	80 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5118 mL
	100 mM	0.0164 mL	0.0819 mL	0.1638 mL	0.4095 mL

Hesperidin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hesperidin520-26-3Hesperetin 7-rutinosideApoptosisAutophagyReactive Oxygen SpeciesEndogenous MetaboliteflavanoneglycosidecitrusinflammatoryantioxidantantiallergicantitumorInhibitorinhibitorinhibit

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Hesperidin (Synonyms: Hesperetin 7-rutinoside)

Customer Review

Other Forms of Hesperidin:

Hesperidin Related Antibodies

12 Publications Citing Use of MCE Hesperidin

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

Hesperidin Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Hesperidin Related Classifications

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