2. Cell Cycle/DNA Damage Anti-infection Autophagy Apoptosis
  3. Topoisomerase Bacterial Fungal Autophagy Antibiotic DNA/RNA Synthesis c-Myc
  4. Idarubicin hydrochloride

Idarubicin hydrochloride  (Synonyms: 4-Demethoxydaunorubicin hydrochloride)

Cat. No.: HY-17381 Purity: 99.94%
COA Handling Instructions

Molarity Calculator

Idarubicin hydrochloride is an anthracycline antileukemic agent. It inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription. Idarubicin hydrochloride inhibits the growth of bacteria and yeasts.

For research use only. We do not sell to patients.

Idarubicin hydrochloride Chemical Structure

Idarubicin hydrochloride Chemical Structure

CAS No. : 57852-57-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 86 In-stock
Solution
10 mM * 1 mL in DMSO USD 86 In-stock
Solid
1 mg USD 28 In-stock
5 mg USD 70 In-stock
10 mg USD 120 In-stock
25 mg USD 260 In-stock
50 mg USD 450 In-stock
100 mg USD 780 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 13 publication(s) in Google Scholar

Other Forms of Idarubicin hydrochloride:

Idarubicin hydrochloride Related Antibodies

Top Publications Citing Use of Products

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Topoisomerase Topo I Topo II

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Idarubicin hydrochloride is an anthracycline antileukemic agent. It inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription. Idarubicin hydrochloride inhibits the growth of bacteria and yeasts.

IC50 & Target[1]

Topoisomerase II

 

In Vitro

The IC50 of Idarubicin hydrochloride is 3.3±0.4 ng/mL on MCF-7 monolayers and 7.9±1.1 ng/mL in multicellular spheroids[1]. Idarubicin hydrochloride has shown a greater cytotoxicity than daunorubicin or doxorubicin in various in vitro systems. This has been attributed to a better ability of idarubicin to induce the formation of topoisomerase II -mediated DNA breaks[2]. Idarubicin hydrochloride is about 57.5-fold and 25-fold more active than doxorubicin and epirubicin, respectively[3]. Idarubicin hydrochloride produces a concentration-dependent reduction in cell growth, with an IC50 value of approximately 0.01 μM. Idarubicin hydrochloride produced a concentration-dependent inhibition of DNA synthesis[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Idarubicin hydrochloride Related Antibodies
Clinical Trial
Molecular Weight

533.95

Formula

C26H28ClNO9
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

OC(C(C(C1=CC=CC=C21)=O)=C3C2=O)=C4[C@H](C[C@@](C(C)=O)(O)CC4=C3O)O[C@@](O[C@@H](C)[C@H]5O)([H])C[C@@H]5N.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (156.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8728 mL 9.3642 mL 18.7283 mL
5 mM 0.3746 mL 1.8728 mL 3.7457 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.94%

COA (256 KB) HNMR (303 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

Stock solutions of idarubicin hydrochloride is dissolved in distilled water (1 mg/mL). MCF-7 monolayer are exposed to idarubicin or its metabolite idarubicinol at 0.01, 0.1, 1, 10, 100, and 1000 ng/mL for 24 hours. Multicellular spheroids are exposed to the same range of idarubicin　and idarubicinol concentration as monolayers (0.01-1000 ng/mL) for 24 h and, in separate experiments, at the drug concentration of 100 ng/mL for 6, 12, 24 and 48 h. The inhibition of cell proliferation is determined by counting the viable cells with an hemocytometer. Results are expressed as percentage of cell survival vs. control cultures[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8728 mL 9.3642 mL 18.7283 mL 46.8209 mL
5 mM 0.3746 mL 1.8728 mL 3.7457 mL 9.3642 mL
10 mM 0.1873 mL 0.9364 mL 1.8728 mL 4.6821 mL
15 mM 0.1249 mL 0.6243 mL 1.2486 mL 3.1214 mL
20 mM 0.0936 mL 0.4682 mL 0.9364 mL 2.3410 mL
25 mM 0.0749 mL 0.3746 mL 0.7491 mL 1.8728 mL
30 mM 0.0624 mL 0.3121 mL 0.6243 mL 1.5607 mL
40 mM 0.0468 mL 0.2341 mL 0.4682 mL 1.1705 mL
50 mM 0.0375 mL 0.1873 mL 0.3746 mL 0.9364 mL
60 mM 0.0312 mL 0.1561 mL 0.3121 mL 0.7803 mL
80 mM 0.0234 mL 0.1171 mL 0.2341 mL 0.5853 mL
100 mM 0.0187 mL 0.0936 mL 0.1873 mL 0.4682 mL
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Idarubicin hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Idarubicin57852-57-04-DemethoxydaunorubicinTopoisomeraseBacterialFungalAutophagyAntibioticDNA/RNA Synthesisc-MycMycleukaemiasMCF-7breast tumorDNA synthesisInhibitorinhibitorinhibit

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