Idoxuridine (Synonyms: 5-Iodo-2′-deoxyuridine; 5-IUdR; IdUrd)

Name: Idoxuridine
Brand: MedChemExpress
SKU: HY-B0307
Rating: 5.0 (4 reviews)

Cat. No.: HY-B0307 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

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Idoxuridine (5-Iodo-2′-deoxyuridine, 5-IUdR, IdUrd) is an iodinated thymidine analogue that competitively inhibits phosphorylases. Idoxuridine can inhibit viral activity, particularly viral eye infections, including herpes simplex keratitis, by inhibiting DNA polymerase and affecting viral replication. Idoxuridine against feline herpesvirus has the IC₅₀ value of 4.3 μM. Idoxuridine shows anti-orthopoxvirus activity.

For research use only. We do not sell to patients.

Idoxuridine Chemical Structure

CAS No. : 54-42-2

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10 mM * 1 mL in DMSO ready for reconstitution	USD 39	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 39	In-stock	Estimated Time of Arrival: December 31
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500 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Idoxuridine:

Idoxuridine hydrate Get quote
Idoxuridine (Standard) Get quote

4 Publications Citing Use of MCE Idoxuridine

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Description

IC₅₀ & Target^[4]

HSV-1

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	> 100 μM Compound: 4; IDU	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 27032331]
HEL	CC₅₀	> 50 μg/mL Compound: IDU	Tested for the cytotoxic concentration, required to reduce cell growth by 50% in human embryonic lung (HEL) cells. Tested for the cytotoxic concentration, required to reduce cell growth by 50% in human embryonic lung (HEL) cells.	[PMID: 7877148]
HEL	IC₅₀	> 50 μg/mL Compound: IDU	Tested for the inhibitory concentration required to reduce cytomegalovirus (CMV) strain Davis plaque formation by 50%. Tested for the inhibitory concentration required to reduce cytomegalovirus (CMV) strain Davis plaque formation by 50%.	[PMID: 7877148]
HEL	IC₅₀	0.17 μg/mL Compound: IDU	Tested for the inhibitory concentration required to reduce TK+ Varicella-Zoster virus (TK+ VZV) strain OKA plaque formation by 50%. Tested for the inhibitory concentration required to reduce TK+ Varicella-Zoster virus (TK+ VZV) strain OKA plaque formation by 50%.	[PMID: 7877148]
HEL	IC₅₀	0.4 μg/mL Compound: IDU	Tested for the 50% inhibitory concentration required to reduce TK+ Varicella-Zoster virus (TK+ VZV) strain YS plaque formation by 50% Tested for the 50% inhibitory concentration required to reduce TK+ Varicella-Zoster virus (TK+ VZV) strain YS plaque formation by 50%	[PMID: 7877148]
HEL	IC₅₀	20 μg/mL Compound: IDU	Tested for the inhibitory concentration required to reduce cytomegalovirus (CMV) strain AD-169 plaque formation by 50%. Tested for the inhibitory concentration required to reduce cytomegalovirus (CMV) strain AD-169 plaque formation by 50%.	[PMID: 7877148]
HEL	IC₅₀	4 μg/mL Compound: IDU	Tested for the inhibitory concentration required to reduce thymidine kinase deficient Varicella-Zoster virus (TK- VZV) strain 07/1 plaque formation by 50%. Tested for the inhibitory concentration required to reduce thymidine kinase deficient Varicella-Zoster virus (TK- VZV) strain 07/1 plaque formation by 50%.	[PMID: 7877148]
HEL	IC₅₀	4 μg/mL Compound: IDU	Tested for the inhibitory concentration required to reduce thymidine kinase deficient Varicella-Zoster virus (TK- VZV) strain YS/R plaque formation by 50%. Tested for the inhibitory concentration required to reduce thymidine kinase deficient Varicella-Zoster virus (TK- VZV) strain YS/R plaque formation by 50%.	[PMID: 7877148]
HFF	CC₅₀	> 260 μM Compound: 5-iodo-2'-deoxyuridine	Cytotoxicity against HFF cells by neutral red uptake assay Cytotoxicity against HFF cells by neutral red uptake assay	[PMID: 16821766]
HFF	EC₅₀	1.1 μM Compound: IDU, idoxuridine	Antiviral activity against TK+ Cowpox virus Delta crmA in HFF cells after 48 hrs by beta-galactosidase assay Antiviral activity against TK+ Cowpox virus Delta crmA in HFF cells after 48 hrs by beta-galactosidase assay	[PMID: 17325220]
HFF	EC₅₀	1.8 μM Compound: IDU, idoxuridine	Antiviral activity against Cowpox virus Brighton in HFF cells after 1 hr by plaque reduction assay Antiviral activity against Cowpox virus Brighton in HFF cells after 1 hr by plaque reduction assay	[PMID: 17325220]
HFF	EC₅₀	2 μM Compound: 5-iodo-2'-deoxyuridine	Antiviral activity against cowpox virus in HFF cells by plaque reduction assay Antiviral activity against cowpox virus in HFF cells by plaque reduction assay	[PMID: 16821766]
HFF	EC₅₀	5.6 μM Compound: IDU, idoxuridine	Antiviral activity against Vaccinia virus in HFF cells after 1 hr by plaque reduction assay Antiviral activity against Vaccinia virus in HFF cells after 1 hr by plaque reduction assay	[PMID: 17325220]
HFF	EC₅₀	6 μM Compound: 5-iodo-2'-deoxyuridine	Antiviral activity against vaccinia virus in HFF cells by plaque reduction assay Antiviral activity against vaccinia virus in HFF cells by plaque reduction assay	[PMID: 16821766]
HFF	EC₅₀	76 μM Compound: IDU, idoxuridine	Antiviral activity against TK- Cowpox virus TK:GFP lacZ in HFF cells after 48 hrs by beta-galactosidase assay Antiviral activity against TK- Cowpox virus TK:GFP lacZ in HFF cells after 48 hrs by beta-galactosidase assay	[PMID: 17325220]
Jurkat	EC₅₀	79.7 μM Compound: 4; IDU	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human Jurkat cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 27032331]
L5178Y	IC₅₀	4.6 μM Compound: IdUrd	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]Leu. In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]Leu.	[PMID: 11101356]
L5178Y	IC₅₀	5.4 μM Compound: IdUrd	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd. In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd.	[PMID: 11101356]
NC-37	CC₅₀	80 μg/mL Compound: IDU	Cytotoxic concentration required to inhibit NC-37 cells by 50% Cytotoxic concentration required to inhibit NC-37 cells by 50%	10.1016/S0960-894X(01)80146-3
PC-3	EC₅₀	> 100 μM Compound: 4; IDU	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 27032331]
PRK	IC₅₀	0.1 μg/mL Compound: 5-I-2'-d Urd	Concentration required to reduce HSV-1 induced cytopathogenicity by 50% in primary rabbit kidney (PRK) cells Concentration required to reduce HSV-1 induced cytopathogenicity by 50% in primary rabbit kidney (PRK) cells	[PMID: 6267280]
PRK	IC₅₀	0.2 μg/mL Compound: 5-I-2'-d Urd	Concentration required to reduce HSV-2 induced cytopathogenicity by 50% in primary rabbit kidney (PRK) cells; 0.2-0.4 Concentration required to reduce HSV-2 induced cytopathogenicity by 50% in primary rabbit kidney (PRK) cells; 0.2-0.4	[PMID: 6267280]
PRK	IC₅₀	0.3 μg/mL Compound: 5-I-2'-d Urd	Concentration required to inhibit incorporation of [1,'2'-3H]dUrd into DNA of primary rabbit kidney (PRK) cells by 50% Concentration required to inhibit incorporation of [1,'2'-3H]dUrd into DNA of primary rabbit kidney (PRK) cells by 50%	[PMID: 6267280]
PRK	IC₅₀	1 μg/mL Compound: 5-I-2'-d Urd	Concentration required to inhibit incorporation of [Me-3H]-dThd into DNA of primary rabbit kidney (PRK) cells by 50% Concentration required to inhibit incorporation of [Me-3H]-dThd into DNA of primary rabbit kidney (PRK) cells by 50%	[PMID: 6267280]
PRK	IC₅₀	200 μg/mL Compound: 5-I-2'-d Urd	Concentration required to reduce TK-HSV-1 (B2006) induced cytopathogenicity by 50% in primary rabbit kidney (PRK) cells Concentration required to reduce TK-HSV-1 (B2006) induced cytopathogenicity by 50% in primary rabbit kidney (PRK) cells	[PMID: 6267280]
Ramos	EC₅₀	47.9 μM Compound: 4; IDU	Cytotoxicity against human Ramos cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human Ramos cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 27032331]
U-87MG ATCC	EC₅₀	> 100 μM Compound: 4; IDU	Cytotoxicity against human U87 cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against human U87 cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 27032331]
Vero	ED₅₀	1.2 μM Compound: IDU	In vitro antiviral activity by means of plaque-inhibition assay with monolayers of Vero cells infected with the MP strain of herpes simplex virus-1 (HSV-1) In vitro antiviral activity by means of plaque-inhibition assay with monolayers of Vero cells infected with the MP strain of herpes simplex virus-1 (HSV-1)	[PMID: 6086926]
Vero	EC₅₀	11.2 μM Compound: IUdR	Antiviral activity against Herpes B virus 24105 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes B virus 24105 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]
Vero	EC₅₀	2.8 μM Compound: IUdR	Antiviral activity against Herpes simplex virus 1 F infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes simplex virus 1 F infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]
Vero	CC₅₀	6.8 μM Compound: IUdR	Cytotoxicity against african green monkey Vero cells after 2 days Cytotoxicity against african green monkey Vero cells after 2 days	[PMID: 17438061]
Vero	EC₅₀	8.5 μM Compound: IUdR	Antiviral activity against Herpes B virus 32425 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes B virus 32425 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]

In Vitro

Idoxuridine (2-10 μM, 72 hours) has the IC₅₀ value of 4.3 μM of antiviral^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Crandell-Reese feline kidney (CRFK) cells
Concentration:	2-10 μM
Incubation Time:	72 hours
Result:	Showed the IC₅₀ value of 4.3 μM.

Cell Cytotoxicity Assay^[1]

Cell Line:	Crandell-Reese feline kidney (CRFK) cells
Concentration:	5-50 μM
Incubation Time:	72 hours
Result:	Reduced by 10.8% relatively in CRFK cells.

In Vivo

Idoxuridine (intraperitoneal injection, 50-200 mg/kg, 3 times, 3 hours interval) can stimulate the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in C3HeB/FeJ female and male mice and A/J male mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C3HeB/FeJ female and male mice and A/J male mice, aged 2 to 4 months^[2]
Dosage:	50-200 mg/kg
Administration:	Intraperitoneal injection, 3 times, 3 hours interval
Result:	Stimulated the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in the dose range of 50-200 mg/kg.

Clinical Trial

Molecular Weight

354.10

Formula

C₉H₁₁IN₂O₅

CAS No.

54-42-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1)N([C@H]2C[C@H](O)[C@@H](CO)O2)C=C(I)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (353.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8241 mL	14.1203 mL	28.2406 mL
5 mM	0.5648 mL	2.8241 mL	5.6481 mL
10 mM	0.2824 mL	1.4120 mL	2.8241 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (280 KB) SDS (557 KB)

COA (252 KB) HNMR (118 KB) RP-HPLC (179 KB) OR (82 KB)

Handling Instructions (2659 KB)

References

[1]. David J Maggs, et al. In vitro efficacy of ganciclovir, cidofovir, penciclovir, foscarnet, idoxuridine, and acyclovir against feline herpesvirus type-1. Am J Vet Res. 2004 Apr;65(4):399-403. [Content Brief]

[2]. D E Griswold, et al. Stimulation of hemolysin plaque-forming cells by idoxuridine. Cancer Res. 1975 Jan;35(1):88-92. [Content Brief]

[3]. Mark N Prichard, et al. Orthopoxvirus targets for the development of antiviral therapies. Curr Drug Targets Infect Disord [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8241 mL	14.1203 mL	28.2406 mL	70.6015 mL
	5 mM	0.5648 mL	2.8241 mL	5.6481 mL	14.1203 mL
	10 mM	0.2824 mL	1.4120 mL	2.8241 mL	7.0602 mL
	15 mM	0.1883 mL	0.9414 mL	1.8827 mL	4.7068 mL
	20 mM	0.1412 mL	0.7060 mL	1.4120 mL	3.5301 mL
	25 mM	0.1130 mL	0.5648 mL	1.1296 mL	2.8241 mL
	30 mM	0.0941 mL	0.4707 mL	0.9414 mL	2.3534 mL
	40 mM	0.0706 mL	0.3530 mL	0.7060 mL	1.7650 mL
	50 mM	0.0565 mL	0.2824 mL	0.5648 mL	1.4120 mL
	60 mM	0.0471 mL	0.2353 mL	0.4707 mL	1.1767 mL
	80 mM	0.0353 mL	0.1765 mL	0.3530 mL	0.8825 mL
	100 mM	0.0282 mL	0.1412 mL	0.2824 mL	0.7060 mL

Idoxuridine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Idoxuridine54-42-25-Iodo-2′-deoxyuridine 5-IUdR IdUrdPhosphataseOrthopoxvirusAntiviral agentsPhosphorylases inhibitorDNA polymeraseHerpesvirusesFeline herpesvirusIodinated thymidine analogueInhibitorinhibitorinhibit

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Idoxuridine (Synonyms: 5-Iodo-2′-deoxyuridine; 5-IUdR; IdUrd)

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Other Forms of Idoxuridine:

Idoxuridine Related Antibodies

4 Publications Citing Use of MCE Idoxuridine

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References

Customer Review

Idoxuridine Related Antibodies

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Complete Stock Solution Preparation Table

Idoxuridine Related Classifications

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