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  5. L-Tryptophan

L-Tryptophan  (Synonyms: Tryptophan; Tryptophane)

Cat. No.: HY-N0623 Purity: 99.99%
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L-Tryptophan (Tryptophan) is an orally active and essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3. L-Tryptophan can promote an increase in stemness and osteogenic ability of BMSCs in vitro and in vivo. L-Tryptophan inhibits cell proliferation and induced cell cycle arrest with high levels.

For research use only. We do not sell to patients.

L-Tryptophan Chemical Structure

L-Tryptophan Chemical Structure

CAS No. : 73-22-3

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10 mM * 1 mL in DMSO
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Based on 6 publication(s) in Google Scholar

Other Forms of L-Tryptophan:

L-Tryptophan Related Antibodies

Top Publications Citing Use of Products

    L-Tryptophan purchased from MedChemExpress. Usage Cited in: Obesity. 2023 Mar 30.  [Abstract]

    L-Trp (0.7 mM; 48 h) enhances the PA (palmitic acid) increased expression of PDX-1, NKX6.1, and MAFA in MIN6 cells. whereas CLP-3094 (CLP; 0.7 μM; 48 h) causes an opposing effect.

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    Description

    L-Tryptophan (Tryptophan) is an orally active and essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3. L-Tryptophan can promote an increase in stemness and osteogenic ability of BMSCs in vitro and in vivo. L-Tryptophan inhibits cell proliferation and induced cell cycle arrest with high levels[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    HepG2 IC50
    17000 μM
    Compound: 13
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 30851693]
    In Vitro

    L-Tryptophan (Tryptophan; 500, 1000 μM; 48 h) with high levels markedly suppresses pTr2 cell proliferation and increases the percentages of cells in the G1 phase of cell cycle[1].
    L-Tryptophan (10 μM; for three weeks) enhances migration, colony formation and osteogenic differentiation of hBMSCs in vitro[2].
    L-Tryptophan (10 μM; for three weeks) decreases mRNA levels of adipogenic markers PPARγ and LPL[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Tryptophan Related Antibodies

    Cell Viability Assay[1]

    Cell Line: pTr2 cell
    Concentration: 500, 1000 μM
    Incubation Time: 48 h
    Result: 500 and 1000 μM groups had decreased cell viability by 7% and 22%, respectively, at 48 h posttreatment.

    Cell Cycle Analysis[1]

    Cell Line: pTr2 cell
    Concentration: 500, 1000 μM
    Incubation Time: 48 h
    Result: The percentage of pTr2 cells in the G1 phase increased from 65.70% (control) to 71.37% (500 mM) and 73.86% (1000 mM).

    Cell Migration Assay [2]

    Cell Line: hBMSCs
    Concentration: 10 μM
    Incubation Time: For three weeks
    Result: Exhibited a higher ability for migration than the control group.

    RT-PCR[2]

    Cell Line: hBMSCs
    Concentration: 10 μM
    Incubation Time: For three weeks
    Result: Decreased mRNA levels of adipogenic markers PPARγ and LPL.
    In Vivo

    L-Tryptophan (Tryptophan; 10, 50 kg/mg/day; Intraperitoneally; for consecutive three weeks) enhances migration, colony formation and osteogenic differentiation of mBMSCs in vivo with 50 mg/kg/day[1].
    L-Tryptophan (50 mg/kg/day; Intraperitoneally; for consecutive three weeks) accelerates bone regeneration in mouse femur surgical defect[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Five-week-old C57BL/6 mice[2]
    Dosage: 10, 50 kg/mg
    Administration: Intraperitoneally; daily; for consecutive three weeks
    Result: The concentration of 50 mg/kg/day induced a significant increase in the number of SSEA-4 positive cells.
    Increased the mRNA levels of Nanog and Oct-4 and the colony-forming ability of mBMSCs with 50 mg/kg/day.
    Clinical Trial
    Molecular Weight

    204.23

    Formula

    C11H12N2O2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@@H](CC1=CNC2=CC=CC=C12)C(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 7.69 mg/mL (37.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (24.48 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.8964 mL 24.4822 mL 48.9644 mL
    5 mM 0.9793 mL 4.8964 mL 9.7929 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.77 mg/mL (3.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.77 mg/mL (3.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 6.25 mg/mL (30.60 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    • Protocol 2

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 20 mg/mL (97.93 mM); Clear solution; Need ultrasonic and warming and heat to 45°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.99%

    SDS (393 KB)

    COA (254 KB) HNMR (130 KB) RP-HPLC (255 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.8964 mL 24.4822 mL 48.9644 mL 122.4110 mL
    5 mM 0.9793 mL 4.8964 mL 9.7929 mL 24.4822 mL
    10 mM 0.4896 mL 2.4482 mL 4.8964 mL 12.2411 mL
    15 mM 0.3264 mL 1.6321 mL 3.2643 mL 8.1607 mL
    20 mM 0.2448 mL 1.2241 mL 2.4482 mL 6.1206 mL
    DMSO 25 mM 0.1959 mL 0.9793 mL 1.9586 mL 4.8964 mL
    30 mM 0.1632 mL 0.8161 mL 1.6321 mL 4.0804 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Tryptophan Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Tryptophan73-22-3Tryptophan TryptophaneOthersOrallyessential amino acidserotoninmelatoninvitamin B3stemnessosteogenicBMSCsproliferationcell cycle arrestInhibitorinhibitorinhibit

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