2. Vitamin D Related/Nuclear Receptor Immunology/Inflammation Metabolic Enzyme/Protease
  3. Progesterone Receptor Androgen Receptor Glucocorticoid Receptor Endogenous Metabolite
  4. Medroxyprogesterone acetate

Medroxyprogesterone acetate  (Synonyms: Medroxyprogesterone 17-acetate; Farlutin)

Cat. No.: HY-B0469 Purity: 99.88%
COA Handling Instructions

Molarity Calculator

Medroxyprogesterone acetate is a widely used synthetic steroid by its interaction with progesterone, androgen and glucocorticoid receptors.

For research use only. We do not sell to patients.

Medroxyprogesterone acetate Chemical Structure

Medroxyprogesterone acetate Chemical Structure

CAS No. : 71-58-9

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10 mM * 1 mL in DMSO
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Based on 17 publication(s) in Google Scholar

Other Forms of Medroxyprogesterone acetate:

Medroxyprogesterone acetate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Medroxyprogesterone acetate is a widely used synthetic steroid by its interaction with progesterone, androgen and glucocorticoid receptors[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Medroxyprogesterone acetate (10 and 0.5 nM, 48 h) inhibits Steroid-deprived HUVEC eNOS expression[2].
Medroxyprogesterone acetate (10 and 0.5 nM, 16 h) inhibits leukocyte adhesion to human endothelial cells (Steroid-deprived HUVECs) by reduing endothelial adhesion molecule (VCAM-1 and ICAM-1 protein) expression[2].
Medroxyprogesterone acetate (10 and 0.5 nM, 2 h) reduces NF-κB nuclear translocation in Steroid-deprived HUVECs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Medroxyprogesterone acetate Related Antibodies

Immunofluorescence[2]

Cell Line: 100 ng/ml LPS treated endothelial cells
Concentration: 10 and 0.5 nM
Incubation Time: 2h
Result: Inhibited NF-κB nuclear translocation.
In Vivo

Medroxyprogesterone acetate (5 mg/kg, oral gavage, rats) shows a Cmax of 377.9 ng/mL, AUC0- 2535.9 ng·h/mL, t1/2 of 10.2 h[3].
Medroxyprogesterone acetate (0.05-0.2 mg/kg/day, p.o., 14 days, rats) increases allopregnanolone levels in all tissues except in the adrenal gland, and affects β-END levels in the hippocampus[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

386.52

Formula

C24H34O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(O[C@@]([C@]12C)(CC[C@@]1([H])[C@@](C[C@H](C)C3=CC4=O)([H])[C@]([C@]3(CC4)C)([H])CC2)C(C)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (25.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 6.25 mg/mL (16.17 mM; ultrasonic and warming and heat to 60°C)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5872 mL 12.9359 mL 25.8719 mL
5 mM 0.5174 mL 2.5872 mL 5.1744 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (2.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 25 mg/mL (64.68 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.88%

COA (252 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.5872 mL 12.9359 mL 25.8719 mL 64.6797 mL
5 mM 0.5174 mL 2.5872 mL 5.1744 mL 12.9359 mL
10 mM 0.2587 mL 1.2936 mL 2.5872 mL 6.4680 mL
15 mM 0.1725 mL 0.8624 mL 1.7248 mL 4.3120 mL
DMSO 20 mM 0.1294 mL 0.6468 mL 1.2936 mL 3.2340 mL
25 mM 0.1035 mL 0.5174 mL 1.0349 mL 2.5872 mL
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Medroxyprogesterone acetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Medroxyprogesterone71-58-9Medroxyprogesterone 17-acetate FarlutinProgesterone ReceptorAndrogen ReceptorGlucocorticoid ReceptorEndogenous MetaboliteNR3C3Inhibitorinhibitorinhibit

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