N6-(2-Phenylethyl)adenosine (Synonyms: N6-Phenethyladenosine; N6-Phenylethyladenosine)

Name: N6-(2-Phenylethyl)adenosine
Brand: MedChemExpress
SKU: HY-101854
Rating: 4.5 (1 reviews)

Cat. No.: HY-101854 Purity: 99.41%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N6-(2-Phenylethyl)adenosine (N6-Phenethyladenosine), an adenosine derivative, is a potent adenosine receptors (AR) agonist with K_i values of 11.8 nM, 30.1 nM, 0.63 nM for rat A₁AR, human A₁AR and hA₃AR, respectively.

For research use only. We do not sell to patients.

N6-(2-Phenylethyl)adenosine Chemical Structure

CAS No. : 20125-39-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 228	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 396	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 708	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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View All Isoforms

Adenosine A₁ receptor (A₁R) Adenosine A_2A receptor (A_2AR) Adenosine A_2B receptor (A_2BR) Adenosine A₃ receptor (A₃R)

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Ki: 11.8 nM (rA₁AR), 30.1 nM (hA₁AR) and 0.63 nM (hA₃AR)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
RD	EC₅₀	1.8 μM Compound: 4	Antiviral activity against Enterovirus 71 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]
RD	EC₅₀	1.9 μM Compound: 4	Antiviral activity against Enterovirus 71 clinical isolate H08300 461#812 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 clinical isolate H08300 461#812 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]
RD	EC₅₀	2.7 μM Compound: 4	Antiviral activity against Enterovirus 71 clinical isolate TW/1956/05 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 clinical isolate TW/1956/05 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]
RD	EC₅₀	3.7 μM Compound: 4	Antiviral activity against Enterovirus 71 isolate TW/2429/04 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 isolate TW/2429/04 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]
RD	EC₅₀	5.6 μM Compound: 4	Antiviral activity against Enterovirus 71 clinical isolate TW/96016/08 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 clinical isolate TW/96016/08 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]
RD	EC₅₀	8.2 μM Compound: 4	Antiviral activity against Enterovirus 71 clinical isolate 11316 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 clinical isolate 11316 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]
RD	EC₅₀	8.3 μM Compound: 4	Antiviral activity against Enterovirus 71 clinical isolate TW/70902/08 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 clinical isolate TW/70902/08 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]

In Vitro

N6-(2-Phenylethyl)adenosine (N6-Phenethyladenosine) inhibits rA₂AR (IC₅₀=560 nM), hA₂AR (IC₅₀=2250 nM) in CHO cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

371.39

Formula

C₁₈H₂₁N₅O₄

CAS No.

20125-39-7

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=CN=C3NCCC4=CC=CC=C4)O1)O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (269.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6926 mL	13.4629 mL	26.9259 mL
5 mM	0.5385 mL	2.6926 mL	5.3852 mL
10 mM	0.2693 mL	1.3463 mL	2.6926 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.41%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (251 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Tchilibon S, et al. Exploring distal regions of the A3 adenosine receptor binding site: sterically constrained N6-(2-phenylethyl)adenosine derivatives as potent ligands. Bioorg Med Chem. 2004 May 1;12(9):2021-34. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6926 mL	13.4629 mL	26.9259 mL	67.3147 mL
	5 mM	0.5385 mL	2.6926 mL	5.3852 mL	13.4629 mL
	10 mM	0.2693 mL	1.3463 mL	2.6926 mL	6.7315 mL
	15 mM	0.1795 mL	0.8975 mL	1.7951 mL	4.4876 mL
	20 mM	0.1346 mL	0.6731 mL	1.3463 mL	3.3657 mL
	25 mM	0.1077 mL	0.5385 mL	1.0770 mL	2.6926 mL
	30 mM	0.0898 mL	0.4488 mL	0.8975 mL	2.2438 mL
	40 mM	0.0673 mL	0.3366 mL	0.6731 mL	1.6829 mL
	50 mM	0.0539 mL	0.2693 mL	0.5385 mL	1.3463 mL
	60 mM	0.0449 mL	0.2244 mL	0.4488 mL	1.1219 mL
	80 mM	0.0337 mL	0.1683 mL	0.3366 mL	0.8414 mL
	100 mM	0.0269 mL	0.1346 mL	0.2693 mL	0.6731 mL

N6-(2-Phenylethyl)adenosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N6-(2-Phenylethyl)adenosine20125-39-7N6-Phenethyladenosine N6-PhenylethyladenosineAdenosine ReceptorP1 receptoradenosinederivativeARA1ARA2ARA3ARInhibitorinhibitorinhibit

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N6-(2-Phenylethyl)adenosine (Synonyms: N6-Phenethyladenosine; N6-Phenylethyladenosine)

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N6-(2-Phenylethyl)adenosine Related Antibodies

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References

Customer Review

N6-(2-Phenylethyl)adenosine Related Antibodies

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Complete Stock Solution Preparation Table

N6-(2-Phenylethyl)adenosine Related Classifications

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