Nitroprusside disodium dihydrate (Synonyms: Sodium nitroprusside dihydrate; Sodium Nitroferricyanide(III) Dihydrate)

Name: Nitroprusside disodium dihydrate
Brand: MedChemExpress
SKU: HY-A0119
Rating: 5.0 (8 reviews)

Cat. No.: HY-A0119 Purity: 99.72%: FAQs
Data Sheet SDS COA Handling Instructions

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Nitroprusside disodium dehydrate (Sodium nitroprusside dihydrate) is a vasodilator that available for the research of acute hypertension, heart failure. Nitroprusside disodium dehydrate induces autophagy in glutathione-depleted osteoblasts. Nitroprusside disodium dehydrate acts as a nitric oxide (NO) donor in a rat intestinal ischemia reperfusion model.

For research use only. We do not sell to patients.

Nitroprusside disodium dihydrate Chemical Structure

CAS No. : 13755-38-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
10 g	USD 66	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Based on 8 publication(s) in Google Scholar

Other Forms of Nitroprusside disodium dihydrate:

Sodium nitroprusside Get quote

8 Publications Citing Use of MCE Nitroprusside disodium dihydrate

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References
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Description

In Vitro

Nitroprusside disodium dehydrate sensitizes human gastric cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

297.95

Formula

C₅H₄FeN₆Na₂O₃

CAS No.

13755-38-9

Appearance

Solid

Color

Reddish brown to dark brown

SMILES

[Na2[Fe(CN)5NO].2H2O]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (335.63 mM; Need ultrasonic)

DMSO : 50 mg/mL (167.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3563 mL	16.7813 mL	33.5627 mL
5 mM	0.6713 mL	3.3563 mL	6.7125 mL
10 mM	0.3356 mL	1.6781 mL	3.3563 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (335.63 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (271 KB) SDS (480 KB)

COA (243 KB) RP-HPLC (243 KB)

Handling Instructions (2659 KB)

References

[1]. J N Cohn, et al. Nitroprusside. Ann Intern Med. 1979 Nov;91(5):752-7. [Content Brief]

[2]. Namazi, H., Sodium nitroprusside as a nitric oxide donor in a rat intestinal ischemia reperfusion model: a novel molecular mechanism. Clinics (Sao Paulo), 2008. 63(3): p. 405. [Content Brief]

[3]. Min Jeong Son, et al. Sodium nitroprusside induces autophagic cell death in glutathione-depleted osteoblasts. J Biochem Mol Toxicol. Sep-Oct 2010;24(5):313-22. [Content Brief]

[4]. Liuqin Yang, et al. Sodium nitroprusside (SNP) sensitizes human gastric cancer cells to TRAIL-induced apoptosis. Int Immunopharmacol. 2013 Oct;17(2):383-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.3563 mL	16.7813 mL	33.5627 mL	83.9067 mL
	5 mM	0.6713 mL	3.3563 mL	6.7125 mL	16.7813 mL
	10 mM	0.3356 mL	1.6781 mL	3.3563 mL	8.3907 mL
	15 mM	0.2238 mL	1.1188 mL	2.2375 mL	5.5938 mL
	20 mM	0.1678 mL	0.8391 mL	1.6781 mL	4.1953 mL
	25 mM	0.1343 mL	0.6713 mL	1.3425 mL	3.3563 mL
	30 mM	0.1119 mL	0.5594 mL	1.1188 mL	2.7969 mL
	40 mM	0.0839 mL	0.4195 mL	0.8391 mL	2.0977 mL
	50 mM	0.0671 mL	0.3356 mL	0.6713 mL	1.6781 mL
	60 mM	0.0559 mL	0.2797 mL	0.5594 mL	1.3984 mL
	80 mM	0.0420 mL	0.2098 mL	0.4195 mL	1.0488 mL
	100 mM	0.0336 mL	0.1678 mL	0.3356 mL	0.8391 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Nitroprusside disodium dihydrate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitroprusside disodium dihydrate13755-38-9Sodium nitroprusside dihydrate Sodium Nitroferricyanide(III) DihydrateAutophagylowerbloodpressureheartfailuresurgerydecreasebleeding aorticvalvestenosisInhibitorinhibitorinhibit

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Nitroprusside disodium dihydrate (Synonyms: Sodium nitroprusside dihydrate; Sodium Nitroferricyanide(III) Dihydrate)

Customer Review

Other Forms of Nitroprusside disodium dihydrate:

Nitroprusside disodium dihydrate Related Antibodies

8 Publications Citing Use of MCE Nitroprusside disodium dihydrate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Nitroprusside disodium dihydrate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Nitroprusside disodium dihydrate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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