Pentamidine isethionate (Synonyms: MP-601205 isethionate)

Name: Pentamidine isethionate
Brand: MedChemExpress
SKU: HY-B0537B
Rating: 5.0 (9 reviews)

Cat. No.: HY-B0537B Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pentamidine isethionate (MP-601205 isethionate) is an antimicrobial agent and interferes with DNA biosynthetics. Pentamidine isethionate inhibits parasite Leishmania infantum with an IC₅₀ of 2.5 μM. Pentamidine isethionate is a potent and selective protein tyrosine phosphatases (PTPases) and phosphatase of regenerating liver (PRL) inhibitor. Pentamidine isethionate has the potential for Gambian trypanosomiasis, antimony-resistant leishmaniasis, and Pneumocystis carinii pneumonia treatment. Antitumor and antibacterial activities.

For research use only. We do not sell to patients.

Pentamidine isethionate Chemical Structure

CAS No. : 140-64-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Pentamidine isethionate:

Pentamidine Get quote
Pentamidine dihydrochloride Get quote
Pentamidine dimesylate Get quote
Pentamidine isethionate (Standard) Get quote

8 Publications Citing Use of MCE Pentamidine isethionate

: Pentamidine isethionate purchased from MedChemExpress. Usage Cited in: BMC Womens Health. 2022 Nov 24;22(1):470. [Abstract]; Pentamidine (2.5, 5 μM; 24-36 h) reduces cell migration in a dose- and time-dependent manner, in Ishikawa and HEC-1A cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Trypanosoma

Leishmania

Cellular Effect

Cell Line	Type	Value	Description	References
J774	IC₅₀	41.6 μM Compound: S1	Cytotoxicity against J774.1 cell line after 48 hrs Cytotoxicity against J774.1 cell line after 48 hrs	[PMID: 16516467]
L6	IC₅₀	5100 nM Compound: Pentamidine	Cytotoxicity against rat L6 cells assessed as cell viability after 70 hrs by MTT assay Cytotoxicity against rat L6 cells assessed as cell viability after 70 hrs by MTT assay	[PMID: 24398295]

In Vitro

Pentamidine (0-10 μg/mL; 6 days; WM9, DU145, C4-2, Hey, WM480, and A549 cells) treatment inhibits the growth of cancer cells in a concentration-dependent manner^[1].
? The cytotoxic properties of Pentamidine isethionate towards the promastigotes of the protozoan parasite Leishmania infantum is determined. The leishmanicidal activity of Pentamidine isethionate is 60 times higher after 72 h of incubation than that of Cisplatin. Pentamidine isethionate induces a higher amount of programmed cell death (PCD) than Cisplatin, which is associated with inhibition of DNA synthesis and cell-cycle arrest in the G2/M phase. Binding of Pentamidine isethionate to calf-thymus DNA (CT-DNA) induces conformational changes in the DNA double helix, consistent with a B-->A transition. The interaction of Pentamidine isethionate with ubiquitin leads to a 6% increase in the beta-sheet content of the protein^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	WM9, DU145, C4-2, Hey, WM480, and A549 cells
Concentration:	0-10 µg/mL
Incubation Time:	6 days
Result:	The growth of all six of the cell lines in culture was inhibited in a concentration-dependent manner with complete growth inhibition of the cell lines occurring at 10 µg/mL.

In Vivo

Pentamidine (0.25 mg/mouse; intramuscular injection; every 2 days; for 4 weeks; athymic nude mice) treatment markedly inhibits the growth of WM9 human melanoma tumors in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Athymic nude mice (6 weeks old) injected with WM9 cells^[1]
Dosage:	0.25 mg/mouse
Administration:	Intramuscular injection; every 2 days; for 4 weeks
Result:	Markedly inhibited the growth of WM9 human melanoma tumors in nude mice.

Clinical Trial

Molecular Weight

592.68

Formula

C₂₃H₃₆N₄O₁₀S₂

CAS No.

140-64-7

Appearance

Solid

Color

White to off-white

SMILES

N=C(C1=CC=C(OCCCCCOC2=CC=C(C(N)=N)C=C2)C=C1)N.OCCS(=O)(O)=O.OCCS(=O)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (168.73 mM; Need ultrasonic)

DMSO : 100 mg/mL (168.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6873 mL	8.4363 mL	16.8725 mL
5 mM	0.3375 mL	1.6873 mL	3.3745 mL
10 mM	0.1687 mL	0.8436 mL	1.6873 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (290 KB) SDS (393 KB)

COA (249 KB) HNMR (276 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Pathak MK, et al. Pentamidine is an inhibitor of PRL phosphatases with anticancer activity. Mol Cancer Ther. 2002 Dec;1(14):1255-64. [Content Brief]

[2]. Nguewa, P.A., et al., Pentamidine is an antiparasitic and apoptotic drug that selectively modifies ubiquitin. Chem Biodivers, 2005. 2(10): p. 1387-400. [Content Brief]

[3]. Sands M, et al. Pentamidine: a review. Rev Infect Dis. 1985 Sep-Oct;7(5):625-34. [Content Brief]

[4]. David C. Bean, et al. Pentamidine: a drug to consider re-purposing in the targeted treatment of multi-drug resistant bacterial infections? J Lab Precis Med 2017;2:49.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.6873 mL	8.4363 mL	16.8725 mL	42.1813 mL
	5 mM	0.3375 mL	1.6873 mL	3.3745 mL	8.4363 mL
	10 mM	0.1687 mL	0.8436 mL	1.6873 mL	4.2181 mL
	15 mM	0.1125 mL	0.5624 mL	1.1248 mL	2.8121 mL
	20 mM	0.0844 mL	0.4218 mL	0.8436 mL	2.1091 mL
	25 mM	0.0675 mL	0.3375 mL	0.6749 mL	1.6873 mL
	30 mM	0.0562 mL	0.2812 mL	0.5624 mL	1.4060 mL
	40 mM	0.0422 mL	0.2109 mL	0.4218 mL	1.0545 mL
	50 mM	0.0337 mL	0.1687 mL	0.3375 mL	0.8436 mL
	60 mM	0.0281 mL	0.1406 mL	0.2812 mL	0.7030 mL
	80 mM	0.0211 mL	0.1055 mL	0.2109 mL	0.5273 mL
	100 mM	0.0169 mL	0.0844 mL	0.1687 mL	0.4218 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Pentamidine isethionate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pentamidine isethionate (Synonyms: MP-601205 isethionate)

Customer Review

Other Forms of Pentamidine isethionate:

Pentamidine isethionate Related Antibodies

8 Publications Citing Use of MCE Pentamidine isethionate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Parasite Isoform Specific Products:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Pentamidine isethionate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Pentamidine isethionate Related Classifications

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