2. Neuronal Signaling Stem Cell/Wnt Apoptosis
  3. Notch Apoptosis
  4. Tangeretin

Tangeretin  (Synonyms: Tangeritin; NSC53909; NSC618905)

Cat. No.: HY-N0133 Purity: 99.27%
COA Handling Instructions

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Tangeretin (Tangeritin), a flavonoid from citrus fruit peels, has been proven to play an important role in anti-inflammatory responses and neuroprotective effects in several disease models, and is a Notch-1 inhibitor.

For research use only. We do not sell to patients.

Tangeretin Chemical Structure

Tangeretin Chemical Structure

CAS No. : 481-53-8

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10 mM * 1 mL in DMSO
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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Tangeretin:

Tangeretin Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Tangeretin (Tangeritin), a flavonoid from citrus fruit peels, has been proven to play an important role in anti-inflammatory responses and neuroprotective effects in several disease models, and is a Notch-1 inhibitor.

IC50 & Target

Notch-1
In Vitro

Tangeretin enhanced the radiosensitivity of GC cells as demonstrated by MTT and colony formation assays. Tangeretin also attenuated radiation-induced EMT, invasion and migration in GC cells, accompanied by a decrease in Notch-1, Jagged1/2, Hey-1 and Hes-1 expressions. Tangeretin triggered the upregulation of miR-410, a tumor-suppressive microRNA. Furthermore, re-expression of miR-410 prevented radiation-induced EMT and cell invasion[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tangeretin Related Antibodies
In Vivo

In this study, we investigated the in vivo anti-RSV activity of tangeretin in 3-week-old male BALB/c mice. A plaque reduction assay and fluorescence quantitative polymerase chain reaction (FQ-PCR) showed that tangeretin inhibited RSV replication in the lung of mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

372.37

Formula

C20H20O7
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(OC)C(OC)=C(OC)C(OC)=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (67.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6855 mL 13.4275 mL 26.8550 mL
5 mM 0.5371 mL 2.6855 mL 5.3710 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  0.5% CMC-Na/saline water

    Solubility: 1 mg/mL (2.69 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.27%

COA (249 KB) LCMS (267 KB)

Handling Instructions (2659 KB)
References
Cell Assay

The effect of tangeretin on RAW264.7 cells was determined using a MTT assay as previously reported.(13) Briefly, RAW264.7 cells (1 × 104 cells/well) were seeded in a 96-well plate for 24 h and treated with different concentrations of tangeretin (6.3–50.0 μM) and dimethyl sulfoxide (DMSO) (vehicle control, 0.01 and 0.1%) for 10 or 48 h. The absorbance was measured at 570 nm using an enzyme immunoassay (EIA) reader (Thermo Scientific, Waltham, MA), and cell viability (%) was calculated as follows: [(absorbance of the test group – absorbance of the blank control)/(absorbance of the control group – absorbance of the blank control)] × 100.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration

Animal administration [2] The mice were maintained in an air-conditioned, pathogen-free room (temperature of 24 ± 2 °C, with a 12 h light/dark cycle from 6:00 am to 6:00 pm) with free access to food and water. Mice were randomly divided into five groups (n = 10) as follows: normal (control), RSV-challenged, and three treatment groups administered 25, 50, or 100 mg/kg/day tangeretin dissolved in saline. The control and RSV-challenged groups received equal volumes of saline. During the experiment, mice in the treatment groups were intragastrically administrated tangeretin for 3 days consecutively before RSV stimulation. Mice were lightly anesthetized with diethyl ether and intranasally challenged with RSV Long strain [6.7 × 106 plaque-forming units (PFU)] on day 4 after tangeretin treatment, while the control group was sham-infected with an equal volume of HEp-2 cell lysate, which was centrifuged under the same conditions as the viral suspensions. The mice were weighed during the experiment and sacrificed on day 5 post-infection after anesthetizing them with chloral hydrate (Figure 1B). The lung tissues were removed and weighed, and the lung index was calculated using the following formula: lung index = lung weight/body weight.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6855 mL 13.4275 mL 26.8550 mL 67.1375 mL
5 mM 0.5371 mL 2.6855 mL 5.3710 mL 13.4275 mL
10 mM 0.2686 mL 1.3428 mL 2.6855 mL 6.7138 mL
15 mM 0.1790 mL 0.8952 mL 1.7903 mL 4.4758 mL
20 mM 0.1343 mL 0.6714 mL 1.3428 mL 3.3569 mL
25 mM 0.1074 mL 0.5371 mL 1.0742 mL 2.6855 mL
30 mM 0.0895 mL 0.4476 mL 0.8952 mL 2.2379 mL
40 mM 0.0671 mL 0.3357 mL 0.6714 mL 1.6784 mL
50 mM 0.0537 mL 0.2686 mL 0.5371 mL 1.3428 mL
60 mM 0.0448 mL 0.2238 mL 0.4476 mL 1.1190 mL
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Tangeretin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tangeretin481-53-8Tangeritin NSC53909 NSC618905NSC53909NSC 53909NSC-53909NSC618905NSC 618905NSC-618905NotchApoptosisInhibitorinhibitorinhibit

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