Triptonide (Synonyms: NSC 165677; PG 492)

Name: Triptonide
Brand: MedChemExpress
SKU: HY-32736
Rating: 5.0 (3 reviews)

Cat. No.: HY-32736 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions

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Triptonide (NSC 165677) is a natural product identified in Tripterygium wilfordii Hook F.. Triptonide is a Wnt signaling inhibitor with an IC₅₀ of appropriately 0.3?nM. Triptonide has immunosuppression, anti-inflammatory, anti-fertility, neuroprotective and anti-lymphoma effects.

For research use only. We do not sell to patients.

Triptonide Chemical Structure

CAS No. : 38647-11-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 290	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 290	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 264	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
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Based on 3 publication(s) in Google Scholar

Other Forms of Triptonide:

Triptonide (Standard) Get quote

3 Publications Citing Use of MCE Triptonide

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Biological Activity
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Description

IC₅₀ & Target

IC50: 0.3 nM (Wnt)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
AGS	IC₅₀	< 20 nM Compound: 8b	Cytotoxicity against human AGS cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human AGS cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
ASPC1	IC₅₀	< 20 nM Compound: 8b	Antiproliferative activity against human ASPC1 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human ASPC1 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
BXPC-3	IC₅₀	< 20 nM Compound: 8b	Antiproliferative activity against human BXPC-3 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human BXPC-3 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
CFPAC-1	IC₅₀	< 20 nM Compound: 8b	Antiproliferative activity against human CFPAC-1 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human CFPAC-1 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
DU-145	IC₅₀	0.01 μM Compound: 8b	Antiproliferative activity against human DU-145 cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human DU-145 cells incubated for 72 hrs by CCK8 assay	[PMID: 33289552]
HGC-27	IC₅₀	< 20 nM Compound: 8b	Cytotoxicity against human HGC-27 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human HGC-27 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
Jurkat	IC₅₀	4 nM Compound: 8b	Antiproliferative activity against human Jurkat cells incubated for 3 to 6 days by alamar blue assay Antiproliferative activity against human Jurkat cells incubated for 3 to 6 days by alamar blue assay	[PMID: 33289552]
LNCaP	IC₅₀	10 nM Compound: 8b	Antiproliferative activity against human LNCaP cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human LNCaP cells incubated for 72 hrs by CCK8 assay	[PMID: 33289552]
MCF7	IC₅₀	0.03 μM Compound: Triptonide	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
MDA-MB-231	IC₅₀	0.03 μM Compound: Triptonide	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
MGC-803	IC₅₀	< 20 nM Compound: 8b	Cytotoxicity against human MGC-803 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human MGC-803 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
NCI-N87	IC₅₀	< 20 nM Compound: 8b	Cytotoxicity against human NCI-N87 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human NCI-N87 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
PANC-1	IC₅₀	< 20 nM Compound: 8b	Cytotoxicity against human Panc-1 cells incubated for 72 hrs by alamar blue assay Cytotoxicity against human Panc-1 cells incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
PANC-1	IC₅₀	47 nM Compound: Triptonide	Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability after 72 hrs Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability after 72 hrs	[PMID: 31889668]
PC-3	IC₅₀	0.012 μM Compound: 8b	Antiproliferative activity against human PC-3 cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human PC-3 cells incubated for 72 hrs by CCK8 assay	[PMID: 33289552]
PC-3	IC₅₀	0.02 μM Compound: Triptonide	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
PC-3	IC₅₀	0.026 μM Compound: 2	Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation by sulforhodamine B assay Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation by sulforhodamine B assay	[PMID: 25467158]
PC-3	IC₅₀	0.6 μM Compound: 2	Antiproliferative activity against human PC3 cells after 72 hrs by SRB assay Antiproliferative activity against human PC3 cells after 72 hrs by SRB assay	[PMID: 20833543]
PC-3	IC₅₀	25 nM Compound: Triptonide	Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by CCK8 assay Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by CCK8 assay	[PMID: 31889668]
Raji	IC₅₀	4 nM Compound: 8b	Antiproliferative activity against human Raji cells incubated for 3 to 6 days by alamar blue assay Antiproliferative activity against human Raji cells incubated for 3 to 6 days by alamar blue assay	[PMID: 33289552]
RKO	IC₅₀	15 nM Compound: 8b	Cytotoxicity against human RKO cells incubated for 72 hrs by Cell Titer-Glo luminescence assay Cytotoxicity against human RKO cells incubated for 72 hrs by Cell Titer-Glo luminescence assay	[PMID: 33289552]
SGC-7901	IC₅₀	< 20 nM Compound: 8b	Cytotoxicity against human SGC-7901 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human SGC-7901 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
SK-OV-3	IC₅₀	0.008 μM Compound: Triptonide	Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
SK-OV-3	IC₅₀	0.008 μM Compound: 2	Antiproliferative activity against human SKOV3 cells after 72 hrs by SRB assay Antiproliferative activity against human SKOV3 cells after 72 hrs by SRB assay	[PMID: 20833543]
SW1990	IC₅₀	< 20 nM Compound: 8b	Antiproliferative activity against human SW1990 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human SW1990 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
SW480	IC₅₀	17 nM Compound: 8b	Cytotoxicity against human SW480 cells incubated for 72 hrs by Cell Titer-Glo luminescence assay Cytotoxicity against human SW480 cells incubated for 72 hrs by Cell Titer-Glo luminescence assay	[PMID: 33289552]
U-251	IC₅₀	0.031 μM Compound: 2	Cytotoxicity against human U251 cells assessed as inhibition of cell proliferation by sulforhodamine B assay Cytotoxicity against human U251 cells assessed as inhibition of cell proliferation by sulforhodamine B assay	[PMID: 25467158]

In Vitro

Triptonide blocks Wnt/β-catenin signaling via C-terminal transactivation domain of β-catenin, and promots apoptosis in Wnt-dependent cancer cells^[1].
Triptonide potently inhibits the proliferation of human B-lymphoma Raji and T-lymphoma Jurkat cells with IC₅₀ of 5.7 nM and 4.8 nM, respectively^[2].
Triptonide (2.5-10 nM; 6 days) significantly suppresses B-lymphoma cell colony-forming capability^[2].
Triptonide (20 nM; 3 days) promotes apoptosis through activation of PARP and caspase 3, but reduction of BCL2 protein levels in the lymphoma cells^[2].
Triptonide (5-10 nM; 72 hours) markedly reduces both total and phosphorylated Lyn proteins, and diminishes Lyn downstream ERK and ATK signal pathways^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	B-lymphoma Raji cells, T-lymphoma Jurkat cells
Concentration:	0-80 nM
Incubation Time:	3 days, 6 days
Result:	Inhibited lymphoma cell tumorigenic capability in a dose-dependent manner.

Apoptosis Analysis^[2]

Cell Line:	Raji cells
Concentration:	5 nM, 10 nM, 20 nM
Incubation Time:	3 days
Result:	Did not significantly induce apoptosis at the effective tumor growth-inhibitory (2.5-10 nM); moderately induced lymphoma cell apoptosis (20 nM).

Western Blot Analysis^[2]

Cell Line:	Raji cells
Concentration:	5 nM, 10 nM, 20 nM
Incubation Time:	3 days
Result:	Did not vigorously activated pro-apoptotic proteins PARP and caspase 3 in lymphoma cells (5-10 nM); significantly activated PARP and caspase 3 (20 nM); significantly reduced anti-apoptotic BCL2 levels.

RT-PCR^[2]

Cell Line:	Raji cells
Concentration:	5 nM, 10 nM
Incubation Time:	72 hours
Result:	Significantly diminished Lyn mRNA levels in the lymphoma cells.

In Vivo

Triptonide (5 mg/kg; i.p.; daily; for 34 days) exerts a strong anti-lymphoma effect in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Eight-week-old female NOD/SCID mice (18-22 g), with 3 x 10⁷ Raji cells xenograft^[2]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection, daily, for 34 days
Result:	Potently inhibited lymphoma cell growth and tumorigenic capability.

Molecular Weight

358.39

Formula

C₂₀H₂₂O₆

CAS No.

38647-11-9

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@]1([C@H]2O1)C(C)C)[C@]34O[C@H]3C[C@@]([H])(C5=C(CC6)C(OC5)=O)[C@@]6(C)[C@]47[C@H]2O7

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (139.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7903 mL	13.9513 mL	27.9026 mL
5 mM	0.5581 mL	2.7903 mL	5.5805 mL
10 mM	0.2790 mL	1.3951 mL	2.7903 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (284 KB) SDS (584 KB)

COA (257 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Jessica Chinison, et al. Triptonide Effectively Inhibits Wnt/β-Catenin Signaling via C-terminal Transactivation Domain of β-catenin. Sci Rep. 2016; 6: 32779. [Content Brief]

[2]. Ping Yang, et al. Triptonide acts as a novel potent anti-lymphoma agent with low toxicity mainly through inhibition of proto-oncogene Lyn transcription and suppression of Lyn signal pathway. Toxicol LettPing Yang. 2017 Aug 15;278:9-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7903 mL	13.9513 mL	27.9026 mL	69.7564 mL
	5 mM	0.5581 mL	2.7903 mL	5.5805 mL	13.9513 mL
	10 mM	0.2790 mL	1.3951 mL	2.7903 mL	6.9756 mL
	15 mM	0.1860 mL	0.9301 mL	1.8602 mL	4.6504 mL
	20 mM	0.1395 mL	0.6976 mL	1.3951 mL	3.4878 mL
	25 mM	0.1116 mL	0.5581 mL	1.1161 mL	2.7903 mL
	30 mM	0.0930 mL	0.4650 mL	0.9301 mL	2.3252 mL
	40 mM	0.0698 mL	0.3488 mL	0.6976 mL	1.7439 mL
	50 mM	0.0558 mL	0.2790 mL	0.5581 mL	1.3951 mL
	60 mM	0.0465 mL	0.2325 mL	0.4650 mL	1.1626 mL
	80 mM	0.0349 mL	0.1744 mL	0.3488 mL	0.8720 mL
	100 mM	0.0279 mL	0.1395 mL	0.2790 mL	0.6976 mL

Triptonide Related Classifications

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Triptonide (Synonyms: NSC 165677; PG 492)

Customer Review

Other Forms of Triptonide:

3 Publications Citing Use of MCE Triptonide

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•Related Screening Libraries:

•Related Small Molecules:

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References

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Triptonide (Synonyms: NSC 165677; PG 492)

Customer Review

Other Forms of Triptonide:

Triptonide Related Antibodies

3 Publications Citing Use of MCE Triptonide

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

Triptonide Related Antibodies

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Complete Stock Solution Preparation Table

Triptonide Related Classifications

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