2. Stem Cell/Wnt Apoptosis Autophagy
  3. Wnt β-catenin Apoptosis Autophagy
  4. Triptonide

Triptonide  (Synonyms: NSC 165677; PG 492)

Cat. No.: HY-32736 Purity: 99.87%
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Triptonide (NSC 165677) is a natural product identified in Tripterygium wilfordii Hook F.. Triptonide is a Wnt signaling inhibitor with an IC50 of appropriately 0.3 nM. Triptonide has immunosuppression, anti-inflammatory, anti-fertility, neuroprotective and anti-lymphoma effects.

For research use only. We do not sell to patients.

Triptonide Chemical Structure

Triptonide Chemical Structure

CAS No. : 38647-11-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 290 In-stock
Solution
10 mM * 1 mL in DMSO USD 290 In-stock
Solid
1 mg USD 68 In-stock
5 mg USD 170 In-stock
10 mg USD 264 In-stock
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Based on 3 publication(s) in Google Scholar

Other Forms of Triptonide:

Triptonide Related Antibodies

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  • Purity & Documentation

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Description

Triptonide (NSC 165677) is a natural product identified in Tripterygium wilfordii Hook F.. Triptonide is a Wnt signaling inhibitor with an IC50 of appropriately 0.3 nM. Triptonide has immunosuppression, anti-inflammatory, anti-fertility, neuroprotective and anti-lymphoma effects[1][2].

IC50 & Target

IC50: 0.3 nM (Wnt)[1]
In Vitro

Triptonide blocks Wnt/β-catenin signaling via C-terminal transactivation domain of β-catenin, and promots apoptosis in Wnt-dependent cancer cells[1].
Triptonide potently inhibits the proliferation of human B-lymphoma Raji and T-lymphoma Jurkat cells with IC50 of 5.7 nM and 4.8 nM, respectively[2].
Triptonide (2.5-10 nM; 6 days) significantly suppresses B-lymphoma cell colony-forming capability[2].
Triptonide (20 nM; 3 days) promotes apoptosis through activation of PARP and caspase 3, but reduction of BCL2 protein levels in the lymphoma cells[2].
Triptonide (5-10 nM; 72 hours) markedly reduces both total and phosphorylated Lyn proteins, and diminishes Lyn downstream ERK and ATK signal pathways[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Triptonide Related Antibodies

Cell Proliferation Assay[2]

Cell Line: B-lymphoma Raji cells, T-lymphoma Jurkat cells
Concentration: 0-80 nM
Incubation Time: 3 days, 6 days
Result: Inhibited lymphoma cell tumorigenic capability in a dose-dependent manner.

Apoptosis Analysis[2]

Cell Line: Raji cells
Concentration: 5 nM, 10 nM, 20 nM
Incubation Time: 3 days
Result: Did not significantly induce apoptosis at the effective tumor growth-inhibitory (2.5-10 nM); moderately induced lymphoma cell apoptosis (20 nM).

Western Blot Analysis[2]

Cell Line: Raji cells
Concentration: 5 nM, 10 nM, 20 nM
Incubation Time: 3 days
Result: Did not vigorously activated pro-apoptotic proteins PARP and caspase 3 in lymphoma cells (5-10 nM); significantly activated PARP and caspase 3 (20 nM); significantly reduced anti-apoptotic BCL2 levels.

RT-PCR[2]

Cell Line: Raji cells
Concentration: 5 nM, 10 nM
Incubation Time: 72 hours
Result: Significantly diminished Lyn mRNA levels in the lymphoma cells.
In Vivo

Triptonide (5 mg/kg; i.p.; daily; for 34 days) exerts a strong anti-lymphoma effect in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Eight-week-old female NOD/SCID mice (18-22 g), with 3 x 107 Raji cells xenograft[2]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection, daily, for 34 days
Result: Potently inhibited lymphoma cell growth and tumorigenic capability.
Molecular Weight

358.39

Formula

C20H22O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@]1([C@H]2O1)C(C)C)[C@]34O[C@H]3C[C@@]([H])(C5=C(CC6)C(OC5)=O)[C@@]6(C)[C@]47[C@H]2O7
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (139.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7903 mL 13.9513 mL 27.9026 mL
5 mM 0.5581 mL 2.7903 mL 5.5805 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

COA (257 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7903 mL 13.9513 mL 27.9026 mL 69.7564 mL
5 mM 0.5581 mL 2.7903 mL 5.5805 mL 13.9513 mL
10 mM 0.2790 mL 1.3951 mL 2.7903 mL 6.9756 mL
15 mM 0.1860 mL 0.9301 mL 1.8602 mL 4.6504 mL
20 mM 0.1395 mL 0.6976 mL 1.3951 mL 3.4878 mL
25 mM 0.1116 mL 0.5581 mL 1.1161 mL 2.7903 mL
30 mM 0.0930 mL 0.4650 mL 0.9301 mL 2.3252 mL
40 mM 0.0698 mL 0.3488 mL 0.6976 mL 1.7439 mL
50 mM 0.0558 mL 0.2790 mL 0.5581 mL 1.3951 mL
60 mM 0.0465 mL 0.2325 mL 0.4650 mL 1.1626 mL
80 mM 0.0349 mL 0.1744 mL 0.3488 mL 0.8720 mL
100 mM 0.0279 mL 0.1395 mL 0.2790 mL 0.6976 mL
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Triptonide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triptonide38647-11-9NSC 165677 PG 492NSC165677NSC-165677PG492PG 492PG-492Wntβ-cateninApoptosisAutophagyBeta cateninTripterygiumwilfordiiHook F.lymphomaanti-cancerLynERKInhibitorinhibitorinhibit

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