2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. AFP464 dihydrochloride

AFP464 dihydrochloride  (Synonyms: NSC710464 dihydrochloride)

Cat. No.: HY-16031B Purity: 99.67%
COA Handling Instructions

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AFP464 (dihydrochloride) (NSC710464 (dihydrochloride)) is the hydrochloride form of AFP464 and is a potent HIF-1α inhibitor with an IC50 value of 0.25 μM. It is also an activator of aryl hydrocarbon receptor (AhR).

For research use only. We do not sell to patients.

AFP464 dihydrochloride Chemical Structure

AFP464 dihydrochloride Chemical Structure

Size Price Stock Quantity
1 mg USD 250 In-stock
5 mg USD 650 In-stock
10 mg USD 1040 In-stock
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100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of AFP464 dihydrochloride:

AFP464 dihydrochloride Related Antibodies

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HIF-1α HIF

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Description

AFP464 (dihydrochloride) (NSC710464 (dihydrochloride)) is the hydrochloride form of AFP464 and is a potent HIF-1α inhibitor with an IC50 value of 0.25 μM. It is also an activator of aryl hydrocarbon receptor (AhR).

Molecular Weight

521.36

Formula

C22H25Cl2F3N4O3
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(NC1=CC=C(C2=CC(C3=C(N)C(F)=C(C)C(F)=C3O2)=O)C=C1F)[C@@H](N)CCCCN.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (95.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 16.67 mg/mL (31.97 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9181 mL 9.5903 mL 19.1806 mL
5 mM 0.3836 mL 1.9181 mL 3.8361 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.67%

COA (247 KB) HNMR (291 KB) LCMS (97 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9181 mL 9.5903 mL 19.1806 mL 47.9515 mL
5 mM 0.3836 mL 1.9181 mL 3.8361 mL 9.5903 mL
10 mM 0.1918 mL 0.9590 mL 1.9181 mL 4.7952 mL
15 mM 0.1279 mL 0.6394 mL 1.2787 mL 3.1968 mL
20 mM 0.0959 mL 0.4795 mL 0.9590 mL 2.3976 mL
25 mM 0.0767 mL 0.3836 mL 0.7672 mL 1.9181 mL
30 mM 0.0639 mL 0.3197 mL 0.6394 mL 1.5984 mL
DMSO 40 mM 0.0480 mL 0.2398 mL 0.4795 mL 1.1988 mL
50 mM 0.0384 mL 0.1918 mL 0.3836 mL 0.9590 mL
60 mM 0.0320 mL 0.1598 mL 0.3197 mL 0.7992 mL
80 mM 0.0240 mL 0.1199 mL 0.2398 mL 0.5994 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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AFP464 dihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AFP464NSC710464AFP 464AFP-464NSC 710464NSC-710464HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHHIF-1α inhibitorInhibitorinhibitorinhibit

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