2. Metabolic Enzyme/Protease Apoptosis
  3. Tyrosinase Apoptosis
  4. Aloesin

Aloesin (Aloeresin) is a tyrosinase inhibitor, and shows anti-inflammatory activity, ultraviolet protection, and antibacterium effects. Aloesin can induce apoptosis and be used in ovarian cancer research.

For research use only. We do not sell to patients.

Aloesin Chemical Structure

Aloesin Chemical Structure

CAS No. : 30861-27-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 440 In-stock
Solution
10 mM * 1 mL in DMSO USD 440 In-stock
Solid
1 mg USD 160 In-stock
5 mg USD 400 In-stock
10 mg USD 640 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Aloesin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Aloesin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Aloesin (Aloeresin) is a tyrosinase inhibitor, and shows anti-inflammatory activity, ultraviolet protection, and antibacterium effects. Aloesin can induce apoptosis and be used in ovarian cancer research[1][2][3].

In Vitro

Aloesin (0-40 μM, 24, 48, and 72 h) inhibits ovarian cancer cell growth in a dose- and time-dependent manner[1].
Aloesin (0-10 μM; 48 h) arrest the cell cycle at S-phase in a dose-dependent manner in SKOV3 cells[1].
Aloesin (0-10 μM; 48 h) promotes cell apoptosis in SKOV3 cells[1].
Aloesin (0-10 μM; 48 h) inhibits the phosphorylation of the MAPK signaling pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Aloesin Related Antibodies

Cell Viability Assay[1]

Cell Line: MCF10A, T47D, MCF7, MDA-MB-468, and MDA-MB-231 cells
Concentration: 0, 2.5, 5, 10, 20, and 40 μM
Incubation Time: 24, 48, and 72 h
Result: Exhibited a concentration-dependent and time-dependent killing of diverse ovarian cancer cell lines.

Cell Viability Assay[1]

Cell Line: SKOV3 cells
Concentration: 2.5-10 μM
Incubation Time: 48 hours
Result: Showed a significantly potent toxic effect with an IC50 value of around 5 μM.

Cell Cycle Analysis[1]

Cell Line: SKOV3 cells
Concentration: 0, 2.5, 5, and 10 μM
Incubation Time: 48 h
Result: Decresed the percentage of cells in the G2/M phase gradually with increasing doses, increased the percentages of cells in the S-phase.
Downregulated the levels of the S-G2/M-related proteins cyclin A, CDK2, and cyclin D1.

Apoptosis Analysis[1]

Cell Line: SKOV3 cells
Concentration: 0, 2.5, 5, and 10 μM
Incubation Time: 48 h
Result: Detected significant proteolytic cleavage of caspase-3, caspase-9, and PARP1.
Increased the expression of Bax and conversely decreased Bcl-2.

Western Blot Analysis[1]

Cell Line: SKOV3 cells
Concentration: 0, 2.5, 5, and 10 μM
Incubation Time: 48 h
Result: Decreased phosphorylated levels of MEK, ERK, MAPK, and JNK.
In Vivo

Aloesin (injection; 20 mg/kg or 40 mg/kg; once daily; 7 w) inhibits tumor growth in a Xenograft model of ovarian cancer[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6-week-old athymic nude mice injected with SKOV3 cells
Dosage: 20 mg/kg or 40 mg/kg
Administration: Injection; 20 mg/kg or 40 mg/kg; once daily; 7 weeks
Result: Reduced volumes and average weights of dissected tumors significantly compared with the control group.
Molecular Weight

394.37

Formula

C19H22O9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC(C)=C(C(C=C(CC(C)=O)O2)=O)C2=C1[C@@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 270 mg/mL (684.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5357 mL 12.6784 mL 25.3569 mL
5 mM 0.5071 mL 2.5357 mL 5.0714 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.25 mg/mL (5.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.25 mg/mL (5.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5357 mL 12.6784 mL 25.3569 mL 63.3922 mL
5 mM 0.5071 mL 2.5357 mL 5.0714 mL 12.6784 mL
10 mM 0.2536 mL 1.2678 mL 2.5357 mL 6.3392 mL
15 mM 0.1690 mL 0.8452 mL 1.6905 mL 4.2261 mL
20 mM 0.1268 mL 0.6339 mL 1.2678 mL 3.1696 mL
25 mM 0.1014 mL 0.5071 mL 1.0143 mL 2.5357 mL
30 mM 0.0845 mL 0.4226 mL 0.8452 mL 2.1131 mL
40 mM 0.0634 mL 0.3170 mL 0.6339 mL 1.5848 mL
50 mM 0.0507 mL 0.2536 mL 0.5071 mL 1.2678 mL
60 mM 0.0423 mL 0.2113 mL 0.4226 mL 1.0565 mL
80 mM 0.0317 mL 0.1585 mL 0.3170 mL 0.7924 mL
100 mM 0.0254 mL 0.1268 mL 0.2536 mL 0.6339 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Aloesin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aloesin30861-27-9AloeresinTyrosinaseApoptosisanti-inflammatory activityultraviolet protectionantibacteriuminduce apoptosisovarian cancerMCF10AT47DMCF7MDA-MB-468MDA-MB-231SKOV3Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Aloesin
Cat. No.:
HY-N2460
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.