2. Epigenetics PI3K/Akt/mTOR
  3. AMPK
  4. AMPK-IN-1

AMPK-IN-1 is an activator of AMPK (EC50: 551 nM for isoform α2β2γ1). AMPK-IN-1 leads to eEF2 phosphorylation in a mTORC1-independent way.

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AMPK-IN-1 Chemical Structure

AMPK-IN-1 Chemical Structure

CAS No. : 1219739-95-3

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Based on 1 publication(s) in Google Scholar

AMPK-IN-1 Related Antibodies

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AMPK-IN-1 is an activator of AMPK (EC50: 551 nM for isoform α2β2γ1). AMPK-IN-1 leads to eEF2 phosphorylation in a mTORC1-independent way[1][2][3].

IC50 & Target[1]

AMPK α2β2γ1

551 nM (EC50)

In Vitro

AMPK-IN-1 (Compound 991) (5 μM, 60 min) induces phosphorylation of acetyl-coenzyme A carboxylase (ACC) in mouse epitrochlearis muscle ex vivo[1].
AMPK-IN-1 (0-10 μM, 60 min) induces eEF2 phosphorylation in MEF cells, and is mainly mTORC1-independent[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AMPK-IN-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: Mouse epitrochlearis muscle
Concentration: 5 μM
Incubation Time: 60 min
Result: Increased phosphorylation of acetyl-coenzyme A carboxylase (ACC).
Molecular Weight

431.87

Formula

C24H18ClN3O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(O)C1=CC(OC2=NC3=CC(Cl)=C(C4=CC5=C(C=C4)C=CN5C)C=C3N2)=CC=C1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (57.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3155 mL 11.5776 mL 23.1551 mL
5 mM 0.4631 mL 2.3155 mL 4.6310 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3155 mL 11.5776 mL 23.1551 mL 57.8878 mL
5 mM 0.4631 mL 2.3155 mL 4.6310 mL 11.5776 mL
10 mM 0.2316 mL 1.1578 mL 2.3155 mL 5.7888 mL
15 mM 0.1544 mL 0.7718 mL 1.5437 mL 3.8592 mL
20 mM 0.1158 mL 0.5789 mL 1.1578 mL 2.8944 mL
25 mM 0.0926 mL 0.4631 mL 0.9262 mL 2.3155 mL
30 mM 0.0772 mL 0.3859 mL 0.7718 mL 1.9296 mL
40 mM 0.0579 mL 0.2894 mL 0.5789 mL 1.4472 mL
50 mM 0.0463 mL 0.2316 mL 0.4631 mL 1.1578 mL
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AMPK-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMPK-IN-11219739-95-3AMPKAMP-activated protein kinaseeEF2mTORC1epitrochlearis muscleMEFACCInhibitorinhibitorinhibit

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