2. Epigenetics Stem Cell/Wnt Protein Tyrosine Kinase/RTK JAK/STAT Signaling
  3. JAK STAT
  4. BD750

BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC50 values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively.

For research use only. We do not sell to patients.

BD750 Chemical Structure

BD750 Chemical Structure

CAS No. : 892686-59-8

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5 mg USD 30 In-stock
10 mg USD 48 In-stock
25 mg USD 96 In-stock
50 mg USD 150 In-stock
100 mg USD 226 In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

BD750 Related Antibodies

Top Publications Citing Use of Products

    BD750 purchased from MedChemExpress. Usage Cited in: FASEB J. 2023 Jan;37(1):e22716.  [Abstract]

    BD750 (24 h) successfully inhibits the JAK–STAT signaling pathway in AML-12 cells. The relevant protein expression levels of the JAK–STAT signaling pathway are detected after transfection of NC-siRNA, P38γ-siRNA, and pEGFP-C1, pEGFP-C1-P38γ and co-cultured with BD750 for 24 h in FFA-treated AML-12 cells.

    BD750 purchased from MedChemExpress. Usage Cited in: FASEB J. 2023 Jan;37(1):e22716.  [Abstract]

    BD750 (0, 2.5, 5, 10, 20, 40 μM; 24 h) decreases AML-12 cells vitality in a concentration-dependent manner.

    View All JAK Isoform Specific Products:

    View All Isoforms
    JAK1 JAK2 JAK3 Tyk2 JAK

    View All STAT Isoform Specific Products:

    View All Isoforms
    STAT3 STAT5 STAT6 STAT1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC50 values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively[1][2].

    IC50 & Target[2]

    JAK3

     

    STAT5

     

    In Vitro

    BD750 inhibits human T cell proliferation stimulated either by anti-CD3/anti-CD28 mAbs or by alloantigen in a dose-dependent manner with IC50 values of 1.1 ± 0.2 μM and 1.3 ± 0.2 μM respectively[1].
    BD750 also inhibits ConA, PMA/ionomycine or alloantigen-induced mouse T cell proliferation and PHA or PMA/ionomycine-induced human T cell proliferation[1].
    BD750 (5 or 20 μM) inhibits the LPS-induced JAK-STAT5 signaling in DC[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BD750 Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Purified immature DCs.
    Concentration: 1, 5 or 20 μM.
    Incubation Time: 12 h.
    Result: Had fewer small dendrites and smaller clusters than typical mDCs (5 or 20 μM).
    At a higher dose significantly reduced the levels of LPS-stimulated IL-6, IL-12, TNF-α, IL-1β and IL-23 production by DCs.
    In Vivo

    BD750 can induce tolerogenic dendritic cells (tolDC) and their function in experimental autoimmune encephalitis (EAE) in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female C57BL/6 mice (10 wks old, 19-21 g)[2].
    Dosage: 20 μM.
    Administration: IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
    Result: Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.
    Molecular Weight

    271.34

    Formula

    C14H13N3OS
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    OC1=C2CCCCC2=NN1C3=NC4=CC=CC=C4S3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (460.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6854 mL 18.4271 mL 36.8541 mL
    5 mM 0.7371 mL 3.6854 mL 7.3708 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6854 mL 18.4271 mL 36.8541 mL 92.1353 mL
    5 mM 0.7371 mL 3.6854 mL 7.3708 mL 18.4271 mL
    10 mM 0.3685 mL 1.8427 mL 3.6854 mL 9.2135 mL
    15 mM 0.2457 mL 1.2285 mL 2.4569 mL 6.1424 mL
    20 mM 0.1843 mL 0.9214 mL 1.8427 mL 4.6068 mL
    25 mM 0.1474 mL 0.7371 mL 1.4742 mL 3.6854 mL
    30 mM 0.1228 mL 0.6142 mL 1.2285 mL 3.0712 mL
    40 mM 0.0921 mL 0.4607 mL 0.9214 mL 2.3034 mL
    50 mM 0.0737 mL 0.3685 mL 0.7371 mL 1.8427 mL
    60 mM 0.0614 mL 0.3071 mL 0.6142 mL 1.5356 mL
    80 mM 0.0461 mL 0.2303 mL 0.4607 mL 1.1517 mL
    100 mM 0.0369 mL 0.1843 mL 0.3685 mL 0.9214 mL
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    BD750 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BD750892686-59-8BD 750BD-750JAKSTATJanus kinaseTcellImmunosuppressantInhibitorinhibitorinhibit

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